Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,146,389 (T)6→7 coding (132/879 nt) pflC → putative pyruvate formate‑lyase 2 activating enzyme PflC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,146,3891.T88.9% 18.9 / ‑2.7 9coding (132/879 nt)pflCputative pyruvate formate‑lyase 2 activating enzyme PflC
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (8/0);  total (9/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01

GGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATG  >  NC_000913/4146163‑4146581
                                                                                                                                                                                                                                    |                                                                                                                                                                                                
ggAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAACCGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTg                                                                                                                                                                                          >  1:44149/1‑236 (MQ=255)
                ncacaAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACCGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGGCCGTGGTGTGCTAATCCg                                                                                                                                                           >  1:61138/2‑251 (MQ=255)
                                                  ccGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCAAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCACCATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGc                                                                                                                          >  1:281166/1‑250 (MQ=255)
                                                                                        aTCCAGGATGACATCATCCGCCGGACAGCGCATCACCTATAACGTTGTGGCAACGCGCCGCGCTGATGTGGCGCGCATTTTAAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGt                                                                                   >  1:126582/1‑251 (MQ=255)
                                                                                              gATGACATCATCCGCCGGACAGCGCATCAGCAGTAAGGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTAAGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGAAAAATCCAGACGGTACGCAGAGAGGCGGAATGTCTGCACTGTGCGCAATGTCTGCGTGATGCg                                                                             >  1:128954/1‑251 (MQ=255)
                                                                                                 gACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGCAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCgg                                                                                                                                             >  1:129303/1‑184 (MQ=255)
                                                                                                                                               cgccgcGATGATGTGGCGCGCATTTTCAAAATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGAGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAg                                                                                                                                   >  1:98257/1‑148 (MQ=255)
                                                                                                                                                                          aaCATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGCCTTTTTTTACAGACTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCACCATCCCGACGGTACGCAGAGAGGCGCAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGACGTTTGAACCGATTGGTCGCGGTATCAGCCTTGACGCTCTGGAACGGGAAGtgatg  >  1:294282/1‑250 (MQ=255)
                                                                                                                                                                                            ntGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTATTACCGGCTGTCCGCATCTTTGCCCGTGGCGTGCTAATCCGGAGTCGCTCTACCGCAAAATcc                                                                                                                                     >  1:62560/2‑101 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                
GGAAGCGCAACAGCGCCCACAAGATTATGCCGGGCTGGTGGTGCGCGTTGCCGGATACAGCGCCTTCTTTGTCGAACTGTCGAAGGAGATCCAGGATGACATCATCCGCCGGACAGCGCATCAGCTGTAACGTTGTGGAAACGCGCCGCGATGATGTGGCGCGCATTTTCAACATTCAGCGTTATTCACTGAATGACGGCGAGGGCATTCGTACGGTGGTCTTTTTTAAAGGCTGTCCGCATCTTTGCCCGTGGTGTGCTAATCCGGAGTCGATCTCCGGCAAAATCCAGACGGTACGCAGAGAGGCGAAATGTCTGCACTGTGCGAAATGTTTGCGTGATGCGGATGAATGCCCCTCCGGGGCGTTTGAACGGATTGGTCGCGATATCAGCCTTGACGCTCTGGAACGGGAAGTGATG  >  NC_000913/4146163‑4146581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: