Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,755,115	G→A	A74T (GCC→ACC)	smpB →	SsrA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,755,115	0	G	A	100.0%	12.4 / NA	7	A74T (GCC→ACC)	smpB	SsrA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/1);  total (6/1)

CACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGGCCGTGGCCTCCACGCATGTGGTGTGCGATCCTACCCGTACCCGCAAGTTACTTCTCAACCAGCGCGAACTGGACTCATTGTACGGTCGCGTCAATCGAGAAGGCTATACCGTAGTGGCGCTCTCCCTGTACTGGAAAAATG  >  NC_000913/2754886‑2755256                                                                                                                                                                                                                                      |                                                                                                                                              cACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTCCGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGACCGTGGCCTCCACGAATgtgg                                                                                                                          >  1:198579/1‑251 (MQ=255)                             caTAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGACCGTGGCCTCCACGCATGTGGTGTGCGATCCTACCCGTACCCGCAAGtt                                                                                              >  1:119284/1‑251 (MQ=255)                                aaaCCTGGTACAGCGACCATCGCGCTTAACAAGCGCGACCGTCACGAATAATTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGAACGGCTGGGAAGTTAAATCCCTGCGCGCACGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGACCGTGGCCTCCACGCATGTGGTGTGCGATCCTACACGTACCCGc                                                                                                   >  1:158583/1‑243 (MQ=255)                                                                                                        gCGGGCCTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGACCGTTGCCTCCACGCATGTGGTGTGCGATCCTACCCGTACCCGCAAGTTACTTCTCAACCAGCGCGAACTGGACTCATTGTACGGTCGCGTCAATCGAGAAGGCTATACCGTAGTGGCGCtctc                   >  1:179260/1‑251 (MQ=255)                                                                                                        gCGGGACTTGCCCTGCAAGGCTGGGCAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGGCAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATAACGCCAATGACCGTGGCCTCCACGCATGTGGTGTGCGATCCTACCCGTACCCGCAAGTTACTTCTCAACCAGCGCGCACTGGACTCATTGTCCGGTCGCGTCAATCGAGAAGGCTATCCCGTAGTGGCGCtctc                   >  1:27241/1‑251 (MQ=255)                                                                                                                 gCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGACCGTGGCCTCCACGCATGTGGTGTGCGATCCTACCCGTCCCCGCAAGTTACTTCTCAACCAGCGCGAACTGGACTCATTGTACGGTCGCGTCAATCGAGAAGGCTATACCGTAGTGGCGCTCTCCCTGTACTg          >  1:37881/1‑251 (MQ=255)                                                                                                                         aGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACATCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGACCGTGGCCTCCACGCATGTGGTGTGCGATCCTACCCGTACCCGCAAGTTACTTCTCAACCAGCGCTAACTGGACTCAGTGTACGGTCGCTTCAATCGAGAAGGCTATACCGTAGTGGCGCTCTCCCTGTACTGGAAAAATg  <  1:151019/251‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                              CACGACGCTTATGACGAAGAAAAAAGCACATAAACCTGGTTCAGCGACCATCGCGCTTAACAAGCGCGCCCGTCACGAATACTTTATCGAAGAAGAGTTCGAAGCGGGACTTGCCCTGCAAGGCTGGGAAGTTAAATCCCTGCGCGCAGGAAAAGCCAATATCAGCGACAGCTACGTCCTTCTGCGTGACGGAGAGGCATTTCTGTTTGGCGCTAACATCACGCCAATGGCCGTGGCCTCCACGCATGTGGTGTGCGATCCTACCCGTACCCGCAAGTTACTTCTCAACCAGCGCGAACTGGACTCATTGTACGGTCGCGTCAATCGAGAAGGCTATACCGTAGTGGCGCTCTCCCTGTACTGGAAAAATG  >  NC_000913/2754886‑2755256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑