Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,747,072	G→A	L242F (CTT→TTT)	ffh ←	signal recognition particle protein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,747,072	0	G	A	100.0%	24.3 / NA	10	L242F (CTT→TTT)	ffh	signal recognition particle protein component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

GGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGACGCATGGACTTGTTTGATCTCGTCCATCATCGCTTCGTCAACGTGCAGACGACCAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTG  >  NC_000913/2746836‑2747300                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     ggCTAATTTCTCTGCCAGCGAGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCAGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGACAACACCGAGCAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGAACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAACGGTACCGTTTCa                                                                                                                                                                                                                         >  1:64485/1‑250 (MQ=255)                                                                            nagaATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAACGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCg                                                                                                                                                                            >  1:130814/2‑220 (MQ=255)                                                                                     cgacgcgaTGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGACCTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAACGGTAACGCTTCCTTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAAt                                                                                                                                            >  1:51180/1‑243 (MQ=255)                                                                                                               ncTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAACGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCTCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCACCCGGGTTAATCGACGCATGGACTTGTTTGATCTCGTCCatcatc                                                                                                          >  1:37958/2‑251 (MQ=255)                                                                                                                           catttttCTCGCCAACACCGAGGAACGTGATCGGTTTGCCAGTTATGGAACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAACGGTAACGCTTCATTGAATGCTTTGGCCGTATTGGTCGCATCCTGACCGGTCATGGCGTCAACCACAAACATGGTTTCAACCGGGTTAATCGACGCATGGACTTGTTTGATCTCGTCCATCATCGCTTCGTCAACg                                                                                              <  1:154634/248‑1 (MQ=255)                                                                                                                                                   aCTTGATCGGTTTGCAAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAACGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGACGCATGGACTTGTTTGATCTCGTCCATCATCGCTTCGTCAACGTGCAGACGACCAGCGGTAGccacc                                                                      <  1:171497/251‑1 (MQ=255)                                                                                                                                                        aTNNNNNTGCNGGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGTATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAACGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCg                                                                                                                                                                            <  2:130814/144‑1 (MQ=255)                                                                                                                                                                                          ncgcACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAAAGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGACGCATGGACTTGTTTGATCTCGTCCATCATCGCTTCGTCAACGTGCAGACGACCAGCGGTATCCCCCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAgc                               >  1:51525/2‑251 (MQ=255)                                                                                                                                                                                             cACCGACGCGGGCATAGCCGTCCAATTTGGTCAACACTACGACGGTAAAAGGTAACGATTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAAACACAAACAGGGATTCAACCGGGTTAATCGACGCATGGACTTGTTTGATCTCGTCCATCATCGCTTCGTCAACGTGCAGACGACCAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGagcc                            >  1:178551/1‑251 (MQ=255)                                                                                                                                                                                                                        tGGTCAACACTACGCCGGTAAACTGTAACGCGTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGACGGATGGACTTGTTTGAGCTCGTCCATCATCGCTTCGTCAACGTGCAGACGACCAGCGGTATCCACCAGCAGCGCGTCGTAGAATTTCAGTTTGGCGTCTTTCAGCGCCGCGTTAACGATATCTACCGGctgctg  <  1:202824/250‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     GGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCGATATCTTCGATCAGCGACAGTACGTCGCCCATGCCGAGAATACGCGACGCGATGCGGTCCGGATGGAACGGCTCCAGCGCCTCAGTTTTCTCGCCAACACCGAGGAACTTGATCGGTTTGCCAGTGATGTGACGAATAGAGAGCGCCGCACCGCCGCGGGCATCGCCGTCCACTTTGGTCAACACTACGCCGGTAAGCGGTAACGCTTCATTGAATGCTTTTGCCGTATTGGCCGCATCCTGACCGGTCATGGCGTCAACCACAAACAGGGTTTCAACCGGGTTAATCGACGCATGGACTTGTTTGATCTCGTCCATCATCGCTTCGTCAACGTGCAGACGACCAGCGGTATCCACCAGCAGCACGTCGTAGAATTTCAGTTTGGCTTCTTTCAGCGCCGCGTTAACGATATCTACCGGCTTCTG  >  NC_000913/2746836‑2747300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑