Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,746,630	G→A	A389V (GCT→GTT)	ffh ←	signal recognition particle protein component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,746,630	0	G	A	100.0%	17.7 / NA	9	A389V (GCT→GTT)	ffh	signal recognition particle protein component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

GCCTGGGGGCATCATACCCTTCATGCTTCTCATCATCTTCGCCATTCCGCCCTTCTTCATTTTCTTCATCATGCGCTGCATGTCGTCGAACTGTTTCAGAAGACGGTTAACGTCCTGCACCTGCATACCGCAACCGGCAGCAATACGGCGTTTACGCGAACCTTTGATGATTTCTGGCTTAGCGCGCTCTTTCATCGTCATCGAGTTGATGATGGCTTCCATACGCACCAGCACTTTATCGTCCATCTGTGACTTGACGTTATCCGGGATCTGCCCCATGCCCGGCAGCTTGCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCG  >  NC_000913/2746449‑2746875                                                                                                                                                                                      |                                                                                                                                                                                                                                                      nccTGGGGGCATCATACCCTTCATGCTTCTCATCATCTTCGCCATTCCGCCCTTCTTCATTTTCTTCATCATGCGCTGCATGTCGTCGAACTGTTTCAGAAGACGGTTAACGTCCTGCACCTGCATACCGCAACCGGCAGCAATACGGCGTTTACGCGAACCTTTGATGATTTCTGGCTTAACGCGCTCTTTCATCGTCATCGAGTTGATGATGGCTTCCATACGCACCAGCACTTTATCGTCCATCtgtg                                                                                                                                                                                  >  1:54366/2‑251 (MQ=255)                                          ccATTCCGCCCTTCTTCATTTTCTTCATCATGCGCTGCATGTCGTCGAACTGTTTCAGAAGCCGGTTAACGTCCTGCACCTGCATACCGCAACCGGCAGCAATACAGCGTTTACGCGAACCTTTGATGATTTCTGGCTTAACGCGCTCTTTCATCGTCATCGAGTTGATGATGGCTTCCATACGCACCAACCCTTTATCGTCCATCTGTGACTTGACGTTATCCGGGATCTGCCCCATGCCCGGCAGCTTg                                                                                                                                         >  1:107073/1‑251 (MQ=255)                                                               tcttcATCATGCGCTGCATGTCGTCGAACTGTTTCAGAAGACGGTTAACGTCCTGCACCTGCATACCGCAACCGGCAGCAATACGGCGTTTACGCGAACCTTTGATGATTTCTGGCTTAACGCGCTCTTTCATCGTCATCGAGTTGATGATGGCTTCCATACGCACCAGCACTTTATCGTCCATCTGTGCCTTGACGTTATCCGGGATCTGCCCCATGCCCGGCGGCTTGCCCATCAGACTAGCCATgcc                                                                                                                     <  1:171306/250‑1 (MQ=255)                                                                                                         ggTTAACGTCCTGCACCTGCATACCGCAACCGGCAGCAATACGGCGTTTACGCGAACCTTTGATGATTTCTGGCTTAACTCGCTCTTTCATCGTCATCGATTTGATGATGGCTTCCATACGCACCAGCACTTTATCGTCCATCTGTGACTTGACGTTAt                                                                                                                                                                      <  1:83062/159‑1 (MQ=255)                                                                                                                                            gcaATACGGCGTTTACGCGAACCTTTGATGATTTCTGGCTTAACGCGCTCTTTCATCGCCATAGAGTTGATGATGGCTACCATCCGTACCAGCACTTTCTCGTCCCTCTCTTACTTGACGTTATCCGGGATCTGCCCCATGCCCGGCAGCATGCCCATCAGACTAGCCATGCCGCCCCTATTTTTCATCTGGCGCAGCTGCTCAAGAAAGCCGTTGAGATCGCAGCCGTCACCTTTTTTCAGCTTGCTGGc                                       >  1:194777/1‑251 (MQ=255)                                                                                                                                                     ngTTTACGCGAACCTTTGATGATTTCTGGCTTAACGCGCTCTTTCATCGTCATCGAGTTGATGATGGCTTCCATACGCACCAGCACTTTATCGTCCATCTGTGACTTGACGTTATCCGGGATCTGCCCCATGCCCGGCAGCTTGCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCg                                                              >  1:23418/2‑219 (MQ=255)                                                                                                                                                                         gatTTCTGGCTTAACGCGCTCTTTCATCGTCATCGAGTTGATGATGGCTTCCATACGCACCAGCACTTTATCGTCCATCTGTGACTTGACGTTATCCGGGATCTGCCCCATGCCCGTCAGCTTGTCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAg                                                                                <  1:96892/181‑1 (MQ=255)                                                                                                                                                                                ngCTTAACGCGCTCTTTCATCGTCATCGAGTTGATGATGGCTTCCATACGCACCAGAACTTTATCGTCAATCTGTGACTTGACGTTATCCGGGATCTGCCCCATGCCCGGCAGCTTGCCAATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTg        >  1:27018/2‑246 (MQ=255)                                                                                                                                                                                  cTTAACGCGCTCTTTCATCGTCATCGAGTCGATGATGGCTTCCATACGCACCAGCACTTTATCGTCCATCTGTGACTTGACGTTATCCGGGATATGCCCCATGCCTGGCAGCTTGCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCg  >  1:66098/1‑250 (MQ=255)                                                                                                                                                                                      |                                                                                                                                                                                                                                                      GCCTGGGGGCATCATACCCTTCATGCTTCTCATCATCTTCGCCATTCCGCCCTTCTTCATTTTCTTCATCATGCGCTGCATGTCGTCGAACTGTTTCAGAAGACGGTTAACGTCCTGCACCTGCATACCGCAACCGGCAGCAATACGGCGTTTACGCGAACCTTTGATGATTTCTGGCTTAGCGCGCTCTTTCATCGTCATCGAGTTGATGATGGCTTCCATACGCACCAGCACTTTATCGTCCATCTGTGACTTGACGTTATCCGGGATCTGCCCCATGCCCGGCAGCTTGCCCATCAGACTAGCCATGCCGCCCATATTTTTCATCTGGCGCAGCTGCTCAAGAAAGTCGTTGAGATCGAAGCCGTCACCTTTTTTCAGCTTGCTGGCTAATTTCTCTGCCTGCGCGCGGTCAACTTTGCTTTCG  >  NC_000913/2746449‑2746875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑