Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,693,412	G→A	S711F (TCT→TTT)	purL ←	phosphoribosylformylglycinamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,693,412	0	G	A	100.0%	19.1 / NA	8	S711F (TCT→TTT)	purL	phosphoribosylformylglycinamide synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (1/7);  total (1/7)

GGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGCTGCGGTCGCCAATGGTCACCAGGAAGGTTTTTTCCGCCACAGTCGGCAGATGCAGCACACGTTTCACCGC  >  NC_000913/2693226‑2693638                                                                                                                                                                                           |                                                                                                                                                                                                                                   gggATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGATAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAAAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCgtagta                                                                                                                                                                    <  1:108088/251‑1 (MQ=255)               cAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAAAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATcn                                                                                                                                                                                       <  1:43991/218‑2 (MQ=255)                     cGGACAAAGCGCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTGCACCAGGGTGGCCTGCCGCCGCCAGCCAGTTGGCTGAAAGTTTGATGCGTTTGATATCGCCAAGTTGTGTTGCGGCGATGTTGGTTAACGCTTCAGCGACCGCCAGACAGGCAAAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAg                                                                                                                                                <  1:97496/251‑1 (MQ=255)                                                                   ccATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAAGTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAAAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTg                                                                                                  <  1:157569/250‑1 (MQ=255)                                                                         tCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAAAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCGCCATCTGATcg                                                                                              <  1:74809/249‑1 (MQ=255)                                                                                    gCCTGCCGCCGCCATCCAGTTGGCGGAAAGGTTGATGCGTTGGAGATCGCCAAGTTGTGTTGCGGCGATTTTGGTTAACGCTTCACCGACCGCCAGACGGGCAAAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCg                                                                                                                                                                                       <  1:33557/149‑1 (MQ=255)                                                                                     ccTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAAAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGGAGTGACCGCGCAGTTAGc                                                                                                                                <  1:61143/203‑1 (MQ=255)                                                                                                                                                                   gCTTCACCGACCGCCAGACGGGCAAAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTGGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGCTGCGGGCGCCAATGGTCACCAGGAAGGGTTTTTCCGCCACAGTCGGCAGATGCAGCACACGTTTCACcgc  >  1:14974/1‑251 (MQ=255)                                                                                                                                                                                           |                                                                                                                                                                                                                                   GGGATCGTCAGGCCCAGCGCCGGACAAAGCTCTTCGCCCACGGCTTTAACGGCTTCATACAGGCCCGCATCTTCACCAGGGTGGCCTGCCGCCGCCATCCAGTTGGCGGAAAGTTTGATGCGTTTGATATCGCCAATTTGTGTTGCGGCGATGTTGGTTAACGCTTCACCGACCGCCAGACGGGCAGAGGCGGCGAAATCCAGCAGCGCAACCGGCGCACGCTCGCCAATCGCCATCGCTTCACCGTAGTAGCTGTCGAGGCTGGCGGTAGTGACCGCGCAGTTAGCGACCGGCACCTGCCACGGCCCCACCATCTGATCGCGCGCTACCATGCCGGTTACGCTGCGGTCGCCAATGGTCACCAGGAAGGTTTTTTCCGCCACAGTCGGCAGATGCAGCACACGTTTCACCGC  >  NC_000913/2693226‑2693638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑