Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,686,511	G→A	G226D (GGT→GAT)	hmp →	nitric oxide dioxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,686,511	0	G	A	100.0%	23.5 / NA	10	G226D (GGT→GAT)	hmp	nitric oxide dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

GGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAATATCTCGGCGTCTGGCTGAAGCCGGAAGGTTTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCATGCGG  >  NC_000913/2686276‑2686720                                                                                                                                                                                                                                            |                                                                                                                                                                                                                  ggtTGGGCAGGTACTCGCGATTTTCGCATTGCGGCTAAAAAACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGCGGCGCAGTGGCCGAATACCGTCCGGGGCAATATCTCGGCGTCTGGCTGAAGCCGGAAGGTTTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGATGGGCAGGTATCCa                                                                                                                                                                                                     >  1:176804/1‑250 (MQ=255)                                  gCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAATATCTCGGCGTCTGGCTGAAGCCGGAAGGTTTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGATGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGt                                                                                                                                                                   >  1:121192/1‑251 (MQ=255)                                   cTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAATATCTCGGCGTCTGGCTGAAGCCGGAAGGTTTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCACAGGCTATCGTATTGCGGTGAAACGCGAAGAGGATGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTc                                                                                                                                                                  >  1:104811/1‑251 (MQ=255)                                                              ccAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAATATCTCGGCGTCTGGCTGAAGCCGGAAGGTTTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGATGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGAt                                                                                                                                       <  1:122925/251‑1 (MQ=255)                                                                                                                ccGGGGCAATATCTCGGCGTCTGGCTGAAGCCGGAAGGTTTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGATGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCgg                                                                                     <  1:162929/251‑1 (MQ=255)                                                                                                                                                     gTTTCCCACCTCAGGAAATTCGTCAGAACTCTTTGACTCGCAAACCGGATGCCAAAGGCTATCGTATTGCGGTGAAACGAGCAGAGGATGGGCAGGTATCCAACTGGTTGCACAATCACGCCCATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGAAGATGACACACCAGTGACGATAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACg                                                >  1:178441/1‑251 (MQ=255)                                                                                                                                                                                              aaaCCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGATGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCATATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCg                                                     <  1:152150/205‑1 (MQ=255)                                                                                                                                                                                                   ggATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGATGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACCAGTGAACTGGTTCCATGCgg  >  1:30931/1‑251 (MQ=255)                                                                                                                                                                                                                                   gcgAAGAGGATGGGCAGGTATCCAACTGGTTGCACNATCNCGCCNATNTTGGCGATGTCGTGANACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGNNANNNCNGTGACGtt                                                                                                 <  2:150954/124‑1 (MQ=255)                                                                                                                                                                                                                                   gcgAAGAGGATGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGCTGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGtt                                                                                                 >  1:150954/1‑124 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                  GGTTGGGAAGGTACTCGCGATTTCCGCATTGTGGCTAAAACACCGCGCAGCGCGCTTATCACCAGCTTCGAACTGGAGCCGGTCGACGGTGGCGCAGTGGCAGAATACCGTCCGGGGCAATATCTCGGCGTCTGGCTGAAGCCGGAAGGTTTCCCACATCAGGAAATTCGTCAGTACTCTTTGACTCGCAAACCGGATGGCAAAGGCTATCGTATTGCGGTGAAACGCGAAGAGGGTGGGCAGGTATCCAACTGGTTGCACAATCACGCCAATGTTGGCGATGTCGTGAAACTGGTCGCTCCGGCAGGTGATTTCTTTATGGCTGTCGCAGATGACACACCAGTGACGTTAATCTCTGCCGGTGTTGGTCAAACGCCAATGCTGGCAATGCTCGACACGCTGGCAAAAGCAGGCCACACAGCACAAGTGAACTGGTTCCATGCGG  >  NC_000913/2686276‑2686720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑