Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,679,297	G→A	A24V (GCC→GTC)	yphF ←	putative ABC transporter periplasmic binding protein YphF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,679,297	0	G	A	100.0%	19.5 / NA	9	A24V (GCC→GTC)	yphF	putative ABC transporter periplasmic binding protein YphF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/6);  total (3/6)

CAACAAAGGTACTTTCTTTCGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCG  >  NC_000913/2679127‑2679513                                                                                                                                                                           |                                                                                                                                                                                                                         caacaaAGGTACTTTCTTTCGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCAGTTCTTTTTCCGCAGCCCTGACAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGAGTACGGGTTGGTCTCATTTTTGTAGGCATAGAGcctcc                                                                                                                                           <  1:179212/250‑1 (MQ=255)                                 tGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGACAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAgg                                                                                                                                     <  1:186368/224‑1 (MQ=255)                                    gCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGACAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAatt                                                                                                        >  1:147779/1‑250 (MQ=255)                                                                                 gCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATTGTCATTTCTTTTTCCGCAGCCCTGACAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTGGGCATAGAGCCTCCTGTATTGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATTAGCTGCGGCGCGTGCCGCTCAAATATTACAGTTa                                                           <  1:184762/250‑1 (MQ=255)                                                                                      cTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGACAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAggg                                                     >  1:18033/1‑251 (MQ=255)                                                                                             nccTGACGCACTGCAGCGTAGTATCTCTGAGTATCACGGTATATTGCGCCAATGGTCCTTTCTTTTTCCGCAGCCCTGACAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTCTTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTg                                              >  1:61499/2‑251 (MQ=255)                                                                                                                                          gcgcCAATGGTCATTTCTTTTTCCGCAGCCCTGACAAATAGTGCGATACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGccn  <  1:133278/250‑2 (MQ=255)                                                                                                                                                   gTCATTTCTTTTTCCGCAGCCCTGACAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAAGAGATCACGGGGTGGTCTCATTTTTGTAGGCATAGATCCTCCTGTAGGGTTTTCACTAACAACCGCTTATTCTAAGTATTTGGTGATGAGCGGCAGCGCGTGCCGCTCAAATAGTACAGTTAATAGGGTGTAGTGAACGCgg                                       <  1:152177/204‑1 (MQ=255)                                                                                                                                                           tttttCCGCAGCCCTGACAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAAcgcgn                                       <  1:52762/196‑2 (MQ=255)                                                                                                                                                                           |                                                                                                                                                                                                                         CAACAAAGGTACTTTCTTTCGAAATATCACCCTGGGCGTTAGTTTCTATTAACTGAACTTGTACTGAGGAATCTTTTGCCGCATCCTGAACGCCCTGACGCACTCCAGCGTAGTATCCCTGAGTATCAAGGTATATTGCGCCAATGGTCATTTCTTTTTCCGCAGCCCTGGCAAATAGTGCGCTACCTAATAGCGTAGCCATTAATAATAGATTACGGGTTGTTCTCATTTTTGTAGGCATAGAGCCTCCTGTAGGGTTTTTATTAACAACGGCTTATTCTAATTATTTTGTGATGAGCGGCAGCGCGTGCCGCTCAAATATTACAGTTAATAGGGTTTAGTGAACGCGGTTAAAAATAAAAGGCATCACGGTGGTGAATAATGCCG  >  NC_000913/2679127‑2679513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑