Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,670,747	C→T	L120L (CTG→TTG)	hcaF →	putative 3‑phenylpropionate/cinnamate dioxygenase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,670,747	0	C	T	100.0%	22.3 / NA	10	L120L (CTG→TTG)	hcaF	putative 3‑phenylpropionate/cinnamate dioxygenase subunit beta
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

CAGCCACCGACAACCTGGATTTTTAATGACACCAAAGACCAGCTGGAGCGGCGAATCGCCCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGG  >  NC_000913/2670567‑2670951                                                                                                                                                                                     |                                                                                                                                                                                                             cAGCCACCGACAACCTGGATTTTTAATGACACCAAAGACCAGCTGGAGCGGCGAATCGCCCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATTTGCTTTAGCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCg                                                                                                                                        <  1:90830/251‑1 (MQ=255)                                                              gTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATTTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATc                                                                           >  1:64376/1‑251 (MQ=255)                                                                    aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTAAATCAACAACAACCAGATAAGAGAAACCGACATCACAAACGTATTTGCTGTGCGGGTAAATTATTTGCTTTATCGGGCACAAAAAAAGCGCGATGAACCATTCTATGTTGGCACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCACGCGGTAATCACTTcc                                                                     >  1:151939/1‑251 (MQ=255)                                                                            aTGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATTTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTgg                                                                                                                                                                >  1:4384/1‑152 (MQ=255)                                                                               ggcTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATAAGCAACAGCCAGATCCGCGACAACGACATCCCAACCGTATTTGCTGTGCGGGTAACTTATTTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGCACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTg                                                            >  1:207937/3‑249 (MQ=255)                                                                                                              gTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATTTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTgn                                                                                                           <  1:52848/171‑2 (MQ=255)                                                                                                                       tcatcatcTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGCGCGGGAAAATTATTTGACTTATCGGGACCCATAAGAGCGCGTTGAAAAATTCTATGTTGGAAAGAGTTTCGCCAAAGATCGCCGTCTGGAAGATGACAACTGGCGCTTGATGGAACGGGATATAGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGAGTACTGTTCTGATGAATCGAATTAATGCGTGTCCCGTTg                  >  1:148776/8‑251 (MQ=255)                                                                                                                       tCAGCAACTGAAAGATAAGCGAAACCGCCATCCCAAACGTATTTGCTGTGCGGGTAAAATATTTACTTTATCGGCGACGAAAAGAGTGCGATGAGACATTATATGTTGGAACGCGATTGGACAAAGGTAGCTGTGTGGAAGATGACAACTGGCGCTTGTTGGACCGGGATATCGTCCTGAATCAAGCGGTAATCACTTCTGATAACCTGAGTGCACTGTTCTGTTGATTCGCATTTATGTGTGTCCTGttt                  >  1:148773/1‑250 (MQ=255)                                                                                                                                                            cGTATTTGCTGTGCGGGTAAATTATTTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAggg  <  1:45692/230‑1 (MQ=255)                                                                                                                                                            cGTATTTGCTGTGCGGGTAAATTATTNGCTTTANNGGNCACAAAAAGAGCGCGATGANACNNNCNNNGNTGGAANGANNTTCGACCAANTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGNATCAAGCGGNNATCACTTCCCATAACCTGAGTNTACNNTNNtnatgntt                                        >  2:45692/1‑189 (MQ=255)                                                                                                                                                                                     |                                                                                                                                                                                                             CAGCCACCGACAACCTGGATTTTTAATGACACCAAAGACCAGCTGGAGCGGCGAATCGCCCGTCTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGTCCCGTTGCGGATGTGCCGGAGGG  >  NC_000913/2670567‑2670951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑