Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	455,339	C→T	D69D (GAC→GAT)	tig →	trigger factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	455,339	0	C	T	100.0%	37.0 / NA	15	D69D (GAC→GAT)	tig	trigger factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/6);  total (9/6)

TTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCG  >  NC_000913/455141‑455566                                                                                                                                                                                                       |                                                                                                                                                                                                                                     ttCAGTTGAAACCACTCAAGGCCTTGTCCGCCGTGTAACAATTACTATCGCTGATGACACAATCGAGAACGCTATTAAACGCGAGCTAGTCAAAGTTGCGCAAAACGTCAGTCTTGACGGCTTCCGCAACGTCAAAGTGCAAATGAAAATCGTTGCTCAGCGTTCTGGCGCGTCTGTACGACAGGACGTTCTGGGTGATCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCgg                                                                                                                                                                                  >  1:120818/1‑251 (MQ=255)    aGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGAGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTCTGGCGCGTCTGTACGCCAGGACGTTCTGGGTGATCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATAAATCCGGCTg                                                                                                                                                                               >  1:193318/1‑251 (MQ=255)     gTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAATACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCTTCTGTACGCCAGGACGTTCTGGGTGATCTGAGGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTgg                                                                                                                                                                              <  1:215488/251‑1 (MQ=255)                     gCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAGTGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCGGGACGTTCTGGGTGATCTGATGAGGCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGTCTggn                                                                                                                                                                             <  1:52273/236‑2 (MQ=255)                                                                cGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGGCTGTACGCCAGGACGTTCTGTGTGATCGGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACTCTGTag                                                                                                                  <  1:116009/251‑1 (MQ=255)                                                                 nagaCCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGATCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTCCTCTGt                                                                                                                    >  1:61758/2‑248 (MQ=255)                                                                                                   cGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGAGATCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGCCTTATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGACGTTGAACTGCAGGGt                                                                               >  1:218549/1‑251 (MQ=255)                                                                                                   cGAAAAAAGGACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGACGCGTCTGTACGCCAGGACGTTCTGGGTGATCTGATAAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGAACTGCAGGGt                                                                               >  1:49482/1‑251 (MQ=255)                                                                                                                             cGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGATCTGATGAGCCGTAACTTCAGTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAAcc                                                     <  1:13080/251‑1 (MQ=255)                                                                                                                                                             ncAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGGGATCTGGTGAGCCGTAACTTCAATGACGCCATAATTAAAGTAAAACTCAATCCGGCTGGCGCACCGACTTATGGTCCGGGCGAAT‑CAGAACAGGGTGAAGACTTAACTTAATCTGTAGAGTTTGAAGTTTATc                                                                                                   >  1:38073/2‑173 (MQ=255)                                                                                                                                                                     aTGGCNNGTNNNTANNCCAGGACGTTCTGGGTGATCTGANGAGCCGTAACTTCATTGACGCCATCATTANANANAAAANNAATNCGGCTGGCGCNCCGACTTATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACNCNGTAGAGTTTGAAGTTTATCCGGAAGTTGAACNGCAGGGTCTGGAAGCGa                                                                     >  2:157578/1‑195 (MQ=255)                                                                                                                                                                     aTGGCGCGTCTGTACGCCAGGACGTTCTTGGTGATCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCGGGCGCACCGACTTATGTTCCGGTCGAATACA‑AGCTTGGTGAAGACTTCTCTTACTCTGTAGAGTTTTAAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGa                                                                     <  1:157578/195‑1 (MQ=255)                                                                                                                                                                                ntACGCCAGGACGTTCTGGGGGATCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAACAAAACAATCCGCCCGGCCCACCGCCTTATATTCCGGCCGCATACA‑AGCTGGGCGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCg  >  1:25489/2‑251 (MQ=255)                                                                                                                                                                                gTACGCCAGGACGTTCTGGGTGATCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAAGCCGGCTGGCGCACCGACATATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGATCTGCAGGGTCTTGAAGCGATCGAATTTGAAAAACCGTTCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCg  <  1:187134/251‑1 (MQ=255)                                                                                                                                                                                       nggACGTTCTGGGTGATCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAAAAAAAATCAATCCGGCTGGCGCACCAACTTCTGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGc                                >  1:25268/2‑214 (MQ=255)                                                                                                                                                                                                       |                                                                                                                                                                                                                                     TTCAGTTGAAACCACTCAAGGCCTTGGCCGCCGTGTAACGATTACTATCGCTGCTGACAGCATCGAGACCGCTGTTAAAAGCGAGCTGGTCAACGTTGCGAAAAAAGTACGTATTGACGGCTTCCGCAAAGGCAAAGTGCCAATGAATATCGTTGCTCAGCGTTATGGCGCGTCTGTACGCCAGGACGTTCTGGGTGACCTGATGAGCCGTAACTTCATTGACGCCATCATTAAAGAAAAAATCAATCCGGCTGGCGCACCGACTTATGTTCCGGGCGAATACA‑AGCTGGGTGAAGACTTCACTTACTCTGTAGAGTTTGAAGTTTATCCGGAAGTTGAACTGCAGGGTCTGGAAGCGATCGAAGTTGAAAAACCGATCGTTGAAGTGACCGACGCTGACGTTGACGGCATGCTGGATACTCTGCG  >  NC_000913/455141‑455566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑