Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,397,444	G→A	Q908* (CAA→TAA)	nuoG ←	NADH:quinone oxidoreductase subunit G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,397,444	0	G	A	100.0%	30.0 / NA	11	Q908* (CAA→TAA)	nuoG	NADH:quinone oxidoreductase subunit G
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

TGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGA  >  NC_000913/2397253‑2397674                                                                                                                                                                                                |                                                                                                                                                                                                                                       tttCCGCAACCAGCTGGCGCGAACCGACCCAGCCAACCAGATTAGGTCCATAACCGTTCTGGAAAAGACCCAGAAGGCAACGTTCGCCAACGCTCATGCATACCCCGCAGGTGACAACAACCAGCACGATCACCACCACTTTGAGGATGGTCAGCAGGATCCCAATCACTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTAAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGAc                                                                                                                                                                              >  1:207202/3‑250 (MQ=255)                            nccAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGc                                                                                                                                                                  >  1:128263/2‑235 (MQ=255)                                                          tCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCGCCAGCAGGATCACCACCGCTTTGAGGATGTTCAGCGGGAGCTTAATCAGTTCCGGGGATATCCAACACATTATTGTGCCTCCTTGAGATCCTCAAGa                                                                                                                                                                                                               <  1:87813/160‑1 (MQ=255)                                                           cTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCggn                                                                                                                                  <  1:43183/236‑2 (MQ=255)                                                                                  gTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGCGGATGGCCAGCAGGATCTCAATCCGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGCTGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCACCCGGCAGCGCGACCGTGTTGCCATCGTAAc                                                                                            >  1:193381/1‑251 (MQ=255)                                                                                                                 gNCAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTc                                                                  >  1:170099/1‑246 (MQ=255)                                                                                                                     nncannnNNAGGNTCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGc                                                                                                                                                                  <  2:128263/144‑1 (MQ=255)                                                                                                                                      caccGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCATCTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTTCCCGGCCGTCACTCCTTCGGc                                                                                                                                  <  1:170574/161‑1 (MQ=255)                                                                                                                                                     ggTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGt                                                                                                                                          <  1:3297/138‑1 (MQ=255)                                                                                                                                                                   cAATCAGTTCCGGTGATATCCAACTAATTATTGTGCCTCCTTGAGATCCTCAAGAGGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCGGCCGTCAGTCCTTCGGCGATTGCAACCGGCAGCGTTACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTCGATGTACGGCTGCGGc               <  1:67141/247‑1 (MQ=255)                                                                                                                                                                              ngTGATATCCAACTCATTATTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGa  >  1:41126/2‑249 (MQ=255)                                                                                                                                                                                                |                                                                                                                                                                                                                                       TGTCCGCAACCAGCTGGAGCGAACCGCCCCAGCCAACACGGTTAGGTCCGTAACGGTTCTGGAACAGACCCAGCAGGCGACGTTCGCCAAAGCTCATGAATGCCCCGCAGGTGACAACCACCAGCAGGATCACCACCGCTTTGAGGATGGTCAGCAGGATCTCAATCAGTTCCGGTGATATCCAACTCATTGTTGTGCCTCCTTGAGATCCTCAAGATGCGCGCCAGCCAGCACCGGAGCAATGCCGGACATACCCATCGGCAAGCCCACCTGCCCTGCCGTCAGTCCTTCGGCGATTTCAACCGGCAGCGTGACCGTGTTGCCATCGTAACTAAAGGAGACGCGTGTACCTGCGTTCACACCCAACTTCGCGGCATCCGCTGGGTTGAGTTTGATGTACGGCTGCGGCATACGGCTCTGGA  >  NC_000913/2397253‑2397674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑