Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,269,634	C→T	intergenic (+67/‑345)	lpxT → / → mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,269,634	0	C	T	100.0%	28.5 / NA	12	intergenic (+67/‑345)	lpxT/mepS	lipid A‑core phosphotransferase/peptidoglycan endopeptidase/peptidoglycan L,D‑carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/8);  total (4/8)

CCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTG  >  NC_000913/2269400‑2269853                                                                                                                                                                                                                                           |                                                                                                                                                                                                                            cccAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACg                                                                                                                                                                                                             >  1:172942/1‑251 (MQ=255)                     acacTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATATTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTa                                                                                                                                                                                         <  1:101664/251‑1 (MQ=255)                                cTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGGTTGCCCTGGGTGTTGCTGACGCCATTCAGTGATCGGTTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCCAAACAAATAACTCACAGTAATTAACATCATCCGGGTTATTTTTATCGGGAGGATAATCCTGATGATGCGAACCGTGTTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGAt                                                                                                                                                                              >  1:31615/1‑251 (MQ=255)                                                                 tGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCTATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTAGTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACGCAAAAAGCATTCTCTAAGTAAATTAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAttn                                                                                                                                              <  1:22692/250‑2 (MQ=255)                                                                                                                                            gAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTTTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCAc                                                                  <  1:155430/251‑1 (MQ=255)                                                                                                                                               aaaaCAAACATTGCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTGATAGTGAGGATAATCCTGATGATGCGCACCTTGTGTTCATCTTGCTAACTCAAAAATCATTCTCTAAGTAAATGAATGGATCGCATTCGTTTCTCGCAATTGTACGTTAATTGACCAACCCCGCTTATTATCTTTCTGTATCGCTTTTTCTTATAAAAAATCATGTAAAAc                                                                                    <  1:201924/230‑1 (MQ=255)                                                                                                                                                    naacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAccc                                                                                                                                      >  1:131483/2‑175 (MQ=255)                                                                                                                                                            ccAAAACAAATAACTCACAGTAATTAACATCAGCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAGTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTGTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtctc                                                   <  1:199827/250‑1 (MQ=255)                                                                                                                                                                              aGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGTGTAATCTCGAACTCGTTTTGCCTcggn                                <  1:54534/251‑2 (MQ=255)                                                                                                                                                                                    tNNNNNCATNAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAccc                                                                                                                                      <  2:131483/143‑1 (MQ=255)                                                                                                                                                                                             caGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGTTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCtn                  <  1:132401/250‑2 (MQ=255)                                                                                                                                                                                                            aGTGAGGCTAATCCTGATGATGCGCACCGTGTATTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGCCCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTtg  >  1:76313/1‑251 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                            CCCAGAGTAATGATTGGCGCACACTGGTTTACTGACATCATTGTCGGTTCGATGACCGTGATATTGATCGGTTTGCCCTGGGTGTTGCTGACGCCATTAAGTGATCGATTAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAACTCGTTTTGCCTCGGCGGTAGATTATCCTCACAGCATATAATTTTG  >  NC_000913/2269400‑2269853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑