Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,250,928	C→T	A30V (GCC→GTC)	nfo →	endonuclease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,250,928	0	C	T	100.0%	27.7 / NA	11	A30V (GCC→GTC)	nfo	endonuclease IV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

GCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTTAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTCGCGCCGCCGAAATCGACGCAACCGCGTTTGCCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGCACCGCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACA  >  NC_000913/2250685‑2251138                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                   nctgATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTTAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGATAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTCGCGCCGCCGAAATCGACGCAACCGCGTTTGTCTTGTTc                                                                                                                                                                                                             >  1:127581/2‑251 (MQ=255)                                                                                                         aaCCCGCTATCATTACCGTTTTCCTCCAGCGGGTTTAACAGGAGTCCTAGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTCGCGCCGCCGAAATCGACGCCACCGCGTTTGTCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGCACCGCTCACGACGCAAACCATCGATGAATTCAAAg                                                                                                                                             >  1:183090/1‑211 (MQ=255)                                                                                                                                       gggTTTAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTCGCGCCGCCGAAATCGACGCAACCGCGTTTGTCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGCACCGCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCgn                                                                       <  1:135534/251‑2 (MQ=255)                                                                                                                                         gTTTAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTTCTGCTGTCGGTCTGGCAAATGCCGCAATTCGTGCCGCCGAAATCGACGCAACCGCGTTTGTCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGCACCGCTCACGACGCAAACCATCGATGAATTCAAAGCCTCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGAc                                                                     <  1:142721/251‑1 (MQ=255)                                                                                                                                                                                                 ntCTGGCAAATGCCGCAATTCGCGCCGCCGAAATCGACGAAACCGCGTTTGTCTTGTTCACAAAAAACCAACGTCAGTGGAGTGCAGCACCGCTCACGACGCAAACCATCGATGAATTCAAAGACGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATACGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGc               >  1:20844/2‑249 (MQ=255)                                                                                                                                                                                                             ncGCAATTCGCGCCGCCGAAATCGACGCAACCGCGTTTGTCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGCACCGCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATAGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACa  >  1:38383/2‑250 (MQ=255)                                                                                                                                                                                                             ccGCAATTCGCGCCGCCGAAATCGACGCAACCGCGTTTGTCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGCACCGCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATc                                                                                    >  1:119735/1‑168 (MQ=255)                                                                                                                                                                                                              nnnnnnnnnngcnnnnNAAANCGCCGCAACCGCGTTTGTCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGCACCGCTCACGACGCAAACCATCGATGAATTCAAACCCGCCTGTGAAAAACATCACTCCACATCGGCGCAAATTCTTccc                                                                                                 <  2:127581/144‑1 (MQ=255)                                                                                                                                                                                                                           gccgaatacGNCNCAACCGCGTTTTTCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGNACCGCTCACGNCCCNNACCANCNANGAATTCAAAGCCGTCTGGGAAAAATATCANTACACATCGGCGCAAATCCTTCCNNACNNNATNNATCTg                                                                                  <  2:158894/148‑1 (MQ=255)                                                                                                                                                                                                                                               gTTTGTCTTGTTCACCAAAAACCAACGTCAGTGGCGTGACGCACCGCTAACGACACAAACCATCGAAGAATTCAAAGCCGACTGAGAAAAATATCACTACACCTCGGCGCAACttc                                                                                                      >  1:150642/1‑116 (MQ=255)                                                                                                                                                                                                                                               gTTTGTCTTGTTCACCAAAAACCAACGNCAGNGGCGNGCNGCACCGCTCACGACGNAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACNNANNNGNGCAAAttc                                                                                                      <  2:150642/116‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                   GCTGATGGCACTGGTACTGTTTGCCTGGCTGATTGTTGGTGGTGGTGCGATTAACTATGTGATTCAAAGCGTCATCGCATAAACCACTACATCTTGCTCCTGTTAACCCGCTATCATTACCGTTTTCCTCCAGCGGGTTTAACAGGAGTCCTCGCATGAAATACATTGGAGCGCACGTTAGTGCTGCTGGCGGTCTGGCAAATGCCGCAATTCGCGCCGCCGAAATCGACGCAACCGCGTTTGCCTTGTTCACCAAAAACCAACGTCAGTGGCGTGCCGCACCGCTCACGACGCAAACCATCGATGAATTCAAAGCCGCCTGTGAAAAATATCACTACACATCGGCGCAAATTCTTCCCCACGACAGTTATCTGATTAACCTCGGACATCCGGTCACTGAAGCTCTGGAAAAATCGCGCGATGCCTTTATAGATGAAATGCAGCGTTGCGAACA  >  NC_000913/2250685‑2251138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑