Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,237,916	C→T	G458G (GGG→GGA)	mglA ←	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,237,916	0	C	T	100.0%	25.5 / NA	12	G458G (GGG→GGA)	mglA	D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

GCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATC  >  NC_000913/2237674‑2238114                                                                                                                                                                                                                                                   |                                                                                                                                                                                                       gCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCCGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCCCTTGcc                                                                                                                                                                                                >  1:32623/1‑251 (MQ=255)     ncAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCCCTTGCCTttc                                                                                                                                                                                            >  1:131805/2‑251 (MQ=255)                     gacgTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCCCTTGCCTTTCTTCGCCAGTTCGGCa                                                                                                                                                                             >  1:50505/1‑250 (MQ=255)                                                                           aCTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGAAAGACGCAGACTTTCGTTTTGCGTTGCTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCCTGCCCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCTCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAATCTTCGCCCCGACATCAATACCGCGCGTCGGTTCAt                                                                                                                      >  1:107944/1‑251 (MQ=255)                                                                                       ccccTAATCTTAAAGGTGCAAAGACACAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCAGTTGAGAGGAGATAATAATAATTCGCTTGCGTGTCTGCGGTAGTGCGGCATGTACTTGAGAAATGTGAAACTTCGCCCGGACATCAATACCGCGCGTCGGTTCATCGAGCATTAata                                                                                                          <  1:99057/251‑1 (MQ=255)                                                                                          cTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACAATTACTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCCCTTGCCTTTCTTCGCCAGTACGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACAGCGAGTCGGTTCATCGAGCATTAATAttt                                                                                                       >  1:159244/1‑251 (MQ=255)                                                                                                                  aagacgcaGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGGGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGAc                                                                               <  1:209511/251‑1 (MQ=255)                                                                                                                                   tttGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCgg                                                                                                                           <  1:5178/190‑1 (MQ=255)                                                                                                                                                                ccGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTg                                 <  1:9240/251‑1 (MQ=255)                                                                                                                                                                                                aCGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCTACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCTTTTTTACCCGCAtn  <  1:42714/250‑2 (MQ=255)                                                                                                                                                                                                 cGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATTCACCTGCATTTCTTCGCCAGTTAGGCACTTAACTGATAAATTTCAAACTTCGCCCCGAcctc                                                                                                                                          >  1:99434/1‑110 (MQ=255)                                                                                                                                                                                                                               tNNNNNGAGNAAATAATAATTCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGAcc                                                                              <  2:131805/143‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                       GCCAGCAAAACTAAAAGAACGACGTAAATACCGCCCTCTTTCAGGTAAGTAAGAAAACTTTTCTTATTTAACGCACTCATGGGAAGCCCCTAATCTTAAAGGTGCAAAGACGCAAGACGCAGAATTTCGTTTTGCGTTGTTGTTTTTGTATCGACAATTCCGGAAACGAGACCATTGCTCATGACCAGAATACGGTCTGTTATCCCTAACAACTCAGGCATTTCAGAGGAGATAATAATAATCCCCTTGCCTTTCTTCGCCAGTTCGGCAATTAACTGATAAATTTCAAACTTCGCCCCGACATCAATACCGCGCGTCGGTTCATCGAGCATTAATATTTCTGGTTGCGTTAGTAGCCAGCGACCAATAATCACCTTTTGCTGATTACCACCGGAGAGCGAACCAATTTGCGTCCGATGACCCGGCGTTTTTACCCGCATC  >  NC_000913/2237674‑2238114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑