Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,219,424	G→A	P20S (CCG→TCG)	osmF ←	glycine betaine ABC transporter periplasmic binding protein OsmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,219,424	0	G	A	100.0%	19.1 / NA	9	P20S (CCG→TCG)	osmF	glycine betaine ABC transporter periplasmic binding protein OsmF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

CTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACGGGGGAAGCCGCCTGTAGCGGCAGGCTCACGGCTGCCAACATAACCAGTGAACCTGCCCAGAGCTTTAAGAGTGGCATGATGCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTG  >  NC_000913/2219208‑2219559                                                                                                                                                                                                                         |                                                                                                                                        cTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACGGGGGAAGCCGCCTGTAGCGACAGGCTCACGGCTGCCAACATAACCAGTGAACCt                                                                                                       >  1:17215/1‑251 (MQ=255)          agaaAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCTCCTTCGGTATCGAGTTTTGAACCGACTTTAACGGGGAAAGCCGCCTGTAGCGACAGGCTCACGGCTGCCAACATAACCAGTGAACCTGCCCAGAGc                                                                                              <  1:107433/251‑1 (MQ=255)              ngcgcCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTAAACCGACTTTAACGGGGGAAGCCGCCTGTAGCGACAGGCTCACGGCTGCCa                                                                                                                        >  1:133698/2‑221 (MQ=255)                                                    cACCGGAAGTAATCGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACGGGGGAAGCCGCCTGTAGCGACAGGCTCACGGCTGCCAACATAACCAGTGAACCTGCCCAGAGCTTTAAGAGTGGCATGATGCTTTCCTCATTCTTTTCCtgttgt                                                    >  1:161266/1‑251 (MQ=255)                                                                       gcgcACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAACTGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACGGGGGAAGCCGCCTGTAGCGACAGGCTCACGGCTGCCAACATACCCAGTGAACCTGCCCAGAGCTTTAAGAGTGGCATGTTGCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTTAgaga                                  >  1:89721/1‑250 (MQ=255)                                                                                                 ncACNTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTAAACCGACTTTAACGGGGGAAGCCGCCTGTAGCGACAGGCTCACGGCTGCCa                                                                                                                        <  2:133698/137‑1 (MQ=255)                                                                                                       tATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACGGGGGAAGCCGCCTGTAGCGACAGGCTCACGGCTGCCAACATAACCAGTGAACCTGCCCAGAGCGTTAAGAGTGGCATGATGCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGTGTTAGCTTg  <  1:47576/250‑1 (MQ=255)                                                                                                                                                                                 aNTTTTGAACCGACTTTAACGGGGGAAGCCGCCTTTANCGANNGGNNGANGGCTg                                                                                                                           <  2:113530/55‑1 (MQ=21)                                                                                                                                                                                                      ggggAAGCCGCCTGTAGCGACAGGCTCACGGCTGCCAACATAACCa                                                                                                               >  1:68653/1‑46 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                        CTTTAAAGAAGAAAGCGCCATTGCCGGTATATTCCGGATAGATATCCAGTTCACCGGAAGTAATCGCCCCGCGCACCACAGGAGTCGTTCCGAGTTGCACTTTATTCACCGTCGGTACGCCGTGGCTTTCCAGCACCTGCAAAATGATATTGCCGAGTAGCGCACCTTCGGTATCGATTTTTGAACCGACTTTAACGGGGGAAGCCGCCTGTAGCGGCAGGCTCACGGCTGCCAACATAACCAGTGAACCTGCCCAGAGCTTTAAGAGTGGCATGATGCTTTCCTCATTCTTTTACTGTTGTTTTCAGCGAATTAAGAGAAAAGCATAGTTGATAATGGCGGGGTTAGCTTG  >  NC_000913/2219208‑2219559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑