Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	453,786	C→T	D128N (GAT→AAT)	yajG ←	putative lipoprotein YajG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	453,786	0	C	T	100.0%	20.2 / NA	9	D128N (GAT→AAT)	yajG	putative lipoprotein YajG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

ATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATC  >  NC_000913/453544‑454005                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                            aTGGGCGGCTACAGGACCGGCTTGCACCGGGACAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATTCGCTTNg                                                                                                                                                                                                                      <  1:168713/250‑1 (MQ=255)                   ggCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATTCGCTTTgg                                                                                                                                                                                                                     <  1:59059/233‑1 (MQ=255)                            cGGGCCAGAGGGAAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGCTGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATTCGCTTTGGTCGCGATGTTGTAGCGCAcagatgga                                                                                                                                                                                           >  1:140321/1‑243 (MQ=255)                               gCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATTCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTggg                                                                                                                                                                                       <  1:87048/251‑1 (MQ=255)                                                aaCGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATTCGCTTTGGTCGCGATGTTGTAGCGCa                                                                                                                                                                                                   >  1:59144/1‑222 (MQ=255)                                                                           ggATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCCCACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCCCCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCAGTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATTCGCTTTGGTCGCGATGGTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTTGCTAACGATGATTTTCAGATTaan                                                                                                                                            <  1:51817/250‑2 (MQ=255)                                                                                       ccTGAGACATATCAGCGATGGTATCGGTCAGCAAACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATTCGCttt                                                                                                                                                                                                                       >  1:65532/1‑163 (MQ=255)                                                                                                                                                                     cACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGTCATTCTTCGCGGTAGCGATGATGGCGATATTCGCTTTTGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTCACGATGCTTTGCAGATTAACCGGGCCATTCGGCCCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGCTCGCGGGAGGCGGTc                                                 >  1:185563/1‑251 (MQ=255)                                                                                                                                                                                                                    ccATTCTGCGCGGTAGCGATGATGGCGATATTCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACAAGTCCGCATACAGTTGGCTAACGATGCTTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATc  >  1:88997/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                            ATGGGCGTCTACAGGCCCGGCTTGCACCGGGCCAGAGGGCAGAAATTAACGCGCGTTCTGCTTGATGAATTCGTGGATGCTGGTGTCCTGAGACATATCAGCGATGGTATCGGTCAGCACACTGTTAACCGCATCGGCGATATTTTTGTTGGAGGCCTGGAACGCACCTTCAACGTTGTAGCTGGCACGATAGTTTTTGGTCATTTTGTTGCCATTCTGCGCGGTAGCGATGATGGCGATATCCGCTTTGGTCGCGATGTTGTAGCGCACGTTGCCCTGGGACACGTCAGCATACAGTTGGCTAACGATGATTTGCAGATTAACCGGGCCATTCGGACCAACCATGTAACCACGCGCGGTCATCTGTTTTTCCAGCACTTCTTGCAGCAGGAAACGCAGATCGCGGGAGGCGGTCAGGGTAACGATTTGATTATCGCGGGTGACTTTTGCCAGCGCCTGATC  >  NC_000913/453544‑454005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑