Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,215,977	G→A	A167V (GCG→GTG)	yehW ←	glycine betaine ABC transporter membrane subunit YehW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,215,977	0	G	A	100.0%	25.7 / NA	11	A167V (GCG→GTG)	yehW	glycine betaine ABC transporter membrane subunit YehW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/6);  total (5/6)

TTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACG  >  NC_000913/2215745‑2216201                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                 ttATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCACCAGCGGTAGCTCGAcc                                                                                                                                                                                                                 <  1:77007/250‑1 (MQ=255)                                                                           ccaGTAACGCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCACCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGc                                                                                                                                      <  1:171380/251‑1 (MQ=255)                                                                                                                  cGCTAAGCCCGCTGATGATGGGCGTACCCAGCGTGATGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCCCCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCACCAGCGGTAGCTCGACATTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACtt                                                                                                                                                                   >  1:76293/1‑183 (MQ=255)                                                                                                                      aaGCCCGATGAGGATGGGCGTACCCAGCGTGCTGGCCCCTCCCGTTGAGGCGATCGTCTCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCACCAGCGGTAGCGCGACCTTACGCACGCGCTGACCACGACTCATTCCCATGCCGGTCTCAACTTCGGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAgg                                                                                            <  1:99816/250‑1 (MQ=255)                                                                                                                          ccGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCACCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCa                                                                                        >  1:1894/1‑250 (MQ=255)                                                                                                                                                              aCCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGTACGCCCGCCAGAATCCCAGGAGGCACCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGc                                                                                                                                                                        >  1:200100/1‑134 (MQ=255)                                                                                                                                                                            cgtcgCCGTACCAATGGTGATAATCCCCGAAGTTCGCCCGCCCGCCAGAAGCACCGGAGCCACCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGTCTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGTAAGTGGCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGAc                                                                <  1:191502/224‑1 (MQ=255)                                                                                                                                                                                                      ccGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCACCAGCGGGAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTc           <  1:159845/251‑1 (MQ=255)                                                                                                                                                                                                              cGCACGCCCGCCAGAATCACCGGAGCCACCAGCGGTAGCTCGACCTTACGCACTAGCTGACCAAGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATAGCTCCCAGACCGGCAAGTGACGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAAc   >  1:149800/1‑251 (MQ=255)                                                                                                                                                                                                               gCACGCCCGACAGCATCACCGGAGCCACCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAAcg  >  1:172638/1‑251 (MQ=255)                                                                                                                                                                                                                                   ggAGCCACCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTGTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCgg                                                                                                                                  <  1:31875/103‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                 TTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCACCGGAGCCGCCAGCGGTAGCTCGACCTTACGCACTCGCTGACCACGACTCATTCCCATACCTTTCGCAACTTCTGTCACGCTGGCATCAATCGCTCCCAGCCCGGCAAGTGTCGCCTGCAGGACGGGCAGCACACCGTAAAGGATCAAGGCGATAATCGCTGGTTGCAGACCAAAGCCGATCACCGGAACGGCGATCGCCAGCACTGCGACGGGCGGAAAAGTCTGTCCAACG  >  NC_000913/2215745‑2216201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑