Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,186,768	C→T	intergenic (+27/‑192)	rcnA → / → rcnB	Ni(2(+))/Co(2(+)) exporter/periplasmic protein involved in nickel/cobalt export

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,186,768	0	C	T	100.0%	31.2 / NA	12	intergenic (+27/‑192)	rcnA/rcnB	Ni(2(+))/Co(2(+)) exporter/periplasmic protein involved in nickel/cobalt export
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/6);  total (6/6)

CCTGACACTGGGCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATCTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCTTTTAGGGCATCCTTTCATTTACACTTTTTACGAAATCATGGGATCACTAACAAAATATCGCTTGTCAGTTATATTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATG  >  NC_000913/2186522‑2186962                                                                                                                                                                                                                                                       |                                                                                                                                                                                                   ccTGACACTGGGCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATTTTc                                                                                                                                                                                                 >  1:142075/1‑250 (MQ=255)                                                              aCCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCACAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATTTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCtttt                                                                                                                                   >  1:213899/1‑251 (MQ=255)                                                                   aGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATTTTATTTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGTTTTTAggg                                                                                                                               <  1:48157/250‑1 (MQ=255)                                                                                 caATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATTTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCTTTTAGGGCATCCTTTCATTTac                                                                                                                <  1:89548/251‑1 (MQ=255)                                                                                       gCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATTTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCTTTTAGGGCATCCTTTCATTTACACtttt                                                                                                          >  1:108713/1‑251 (MQ=255)                                                                                         gTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGAGGATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATTTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCTTTTAGGGCATCCTTTCATTTACACttttt                                                                                                         >  1:93452/1‑250 (MQ=255)                                                                                                                                                                                       atatGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGTCTTGCATGTTATTTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGTTCGCTAAGGATGCTTTTAGGGCATCCTTTCATTTACACTTTTTACGTAATCATGGGATCACTAACAAAAGATCGCTTGTCAGTTATATTGTATGGCAGGAAAGATATGCGACTGATATGACAGATCCCCAAAGTGgag          <  1:180386/251‑1 (MQ=255)                                                                                                                                                                                       atatGGGCGTACATGGCGTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATTTTCATCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCTTTTAGGGCATCCGTTCATTGACACTTTTGACTAAAGCATGGGATCACTAACAAAAGATCGCTTGTCATTTATATTTTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGgag          <  1:144908/251‑1 (MQ=255)                                                                                                                                                                                                       cTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATTTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCTTTTAGGGCATCCTTTCATTTACACTTTTTACGAAATCATGGGATCACt                                                                                      <  1:16725/159‑1 (MQ=255)                                                                                                                                                                                                                              ncAATCCTTTAGGCTTGCATGTTATTTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCTTTTAGGGCATCCTTTCATTTACACTTTTTACGAAATCATGGGATCACTAACAAAATATCGCTTGTCAGTTATATTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATg  >  1:132271/2‑220 (MQ=255)                                                                                                                                                                                                                                     tttATGCTTGCATGTTATTGTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGActctct                                                                                                                                                      <  1:35850/65‑1 (MQ=255)                                                                                                                                                                                                                                     tttAGGCTTGANTGTTATTTTCAGCCCAGGATGATTCTTANATCAGCTATTTNCGCTGNnncnnn                                                                                                                                                      >  2:35850/1‑62 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                   CCTGACACTGGGCGCAACACTGGTCGTCAGTTTCAGCATTGGCCTGGCGTTAACGCTTGTCACCGTAGGCGTTGGCGCAGCAATCAGCGTTCAGCAGGTCGCAAAACGCTGGAGCGGATTTAACACTCTCGCTAAACGCGCCCCCTATTTTTCCAGTCTGTTGATTGGCTTAGTCGGTGTGTATATGGGCGTACATGGCTTCATGGGCATAATGCGATAACTCAATCCTTTAGGCTTGCATGTTATCTTCAGCCCAGGATGATTCTTAAATCAGCTATTTCCGCTGACTCTCTGCTCGCTAAGGATGCTTTTAGGGCATCCTTTCATTTACACTTTTTACGAAATCATGGGATCACTAACAAAATATCGCTTGTCAGTTATATTGTATGGCAGGAAAGATATGCGACTGATATTACAGATCCCCAAAGTGGAGAGTTTATG  >  NC_000913/2186522‑2186962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑