Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,172,238	A→G	S212S (AGT→AGC)	gatD ←	galactitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,172,238	0	A	G	90.0%	17.0 / ‑5.0	10	S212S (AGT→AGC)	gatD	galactitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (3/6);  minor base T (1/0);  total (4/6)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGTCAGCTCTTTACGCAATATTTTGCCAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACCGGCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGC  >  NC_000913/2172026‑2172453                                                                                                                                                                                                                     |                                                                                                                                                                                                                        aCCGTCAGCTCTTTACGCAATATTTTGCCAAACGTTGCCCATGTTAAACCCAGATCCTGCTGCAACGTCACCACCACAGCCAGTACGACATGAGGACCGGCCATCTCTACCACCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGCGGATAAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGCCGCGCTCCTTTCGCTTCTGTTAAATGTTTGCATCGCACCGACAGATTTtgca                                                                                                                                                                                     >  1:144419/1‑248 (MQ=255)     tCAGCTCTTTACGCAATATTTTGCCAAACGTTGCCGATGTTAAATGCAGCTCCTGATGCAACGTGCCCACCAGCGCCAGCTGGGCATGAGGACCGGCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATTAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAAATGTTTGCATCGCACCGAAAGATTTtgccagtgcc                                                                                                                                                                               >  1:219836/1‑251 (MQ=255)                                                     gATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACCGGCCATCTCTAACGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGCGGATAAGCTGATTAAAGCGCAGTTCGCGTACAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAAATGTTTGCATCGCACCGAACGATTTTGCCAGAGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTccc                                                                                                                                  >  1:163217/1‑248 (MQ=255)                                                      aTCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCAGGAGGACCGGCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAgcg                                                                                                                              <  1:192337/251‑1 (MQ=255)                                                            aTGCAACTGGCCCCCCAGCGCCTGTTGGGCATTAGGTCCGGCAATCTCTTCCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGGCTCGAGGATAAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAAATTTTTGCATCGCACCGAAAGATTTTGCTAGTGCCATTTTTTCTGAACTAATGTCGATCGCCGTCACTCTCTTTGCTCCCAGCGCGACAg                                                                                                                        <  1:196079/251‑1 (MQ=255)                                                                             gcgcCAGTTGGGCATGAGGACCGGCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCcagc                                                                                                       <  1:60056/251‑1 (MQ=255)                                                                             gcgcCAGTGGGGCATGGGGACCGGCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAAATGTTTGCATCGCACCGAAAGATTGTGCCAGTGCCAGTTTTTCTGAACTAATGTCTATCGTCGTCACTCTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCcagc                                                                                                       <  1:192345/251‑1 (MQ=255)                                                                                                                                                     ggATAAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAACTGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAg                                    >  1:72811/1‑246 (MQ=255)                                                                                                                                                                       gcAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACGAATGTCGATCGCCGTCACACTCTTTGCTCCCAGcgcg                                                                                                                            <  1:188619/140‑1 (MQ=255)                                                                                                                                                                                  aaaaCGCTCTGCATTTGCGGCGCGCTCATTTCGCTGCTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGc  <  1:65465/251‑1 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                        ACCGTCAGCTCTTTACGCAATATTTTGCCAAACGTTGCCGATGTTAAATGCAGATCCTGATGCAACGTGCCCACCAGCGCCAGTTGGGCATGAGGACCGGCAATCTCTACCGCCAGTTCGACAGTTTGCGGTACGCCAGCCGTCTCGAGGATAAGCTGATTAAAGCGCAGTTCGCGTAAAACGCTCTGCATTTGCGGCGCGCTCATTTCGCTACTGTTAAATGTTTGCATCGCACCGAAAGATTTTGCCAGTGCCAGTTTTTCTGAACTAATGTCGATCGCCGTCACACTCTTTGCTCCCAGCGCGACAGCGCACTGAATGGCCAGCAGGCCAATGGTTCCGGCACCAATAATAATAACGTTTTTATTCTCACAACCTTGCGCTAAATGAAAAGCATGCAGACCAACGGTAATCGGCTCAATAAAAGC  >  NC_000913/2172026‑2172453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑