Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,165,680 G→A E5K (GAA→AAA)  trhP → tRNA wobble base hydroxylation protein TrhP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,165,6800GA92.9% 35.0 / NA 14E5K (GAA→AAA) trhPtRNA wobble base hydroxylation protein TrhP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (4/9);  minor base C (1/0);  total (5/9)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACC  >  NC_000913/2165439‑2165918
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              
aCGCCACCGCGCAATCTCGTGAAACCGGAATTGCTTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTCCCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGGGGGGGCACTCCCCTAACCGCTTCATCAGGGGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAATCCGAAACTCCtt                                                                                                                                                                                                                                        <  1:69307/250‑1 (MQ=255)
                  gTGAAACCGGAATTGCTTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGACCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAACGTGGGGGCACTCCCCTCACCGCTTCATCAGGGGAAGCGGATCTGACCTGTCATCAGCACGAGAGAATTATGTTTAAACCGCAACTCCTTTCCCCGGCGGGAACGCTg                                                                                                                                                                                                                      >  1:161259/1‑250 (MQ=255)
                           gAATTGCCTCCGTTAAAGACAATGGCACAAGCCAGACGGTGAAAGACAATACGTCATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGCGCCCCCCACAGCGCTACAATGCCCGCCCTTACAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGAACCTCATTTCCCCGGCGGGAACGATGACAAATATGc                                                                                                                                                                                                            >  1:33937/1‑251 (MQ=255)
                                              cAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGAATCTCCTTTCCCCGGCGGGAACGCTGAAAAAGATGCGTTACGCTTTCGCTTATgg                                                                                                                                                                                         <  1:70471/251‑1 (MQ=255)
                                                   gCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCGTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTTTCATCAGAACGAGAGAATTATGTTTAAACCGAAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAg                                                                                                                                                                                    <  1:218924/251‑1 (MQ=255)
                                                                               gTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATTCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGAAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGACAGCCGCGTTa                                                                                                                                                        <  1:5950/251‑1 (MQ=255)
                                                                                                   gggTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGAAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTGATGTCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGcaac                                                                                                                                     <  1:66616/250‑1 (MQ=255)
                                                                                                                          aCCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGAAACTCCTTTCCCCGGCGGGAACGCTGaaaaa                                                                                                                                                                                                                 >  1:105960/1‑151 (MQ=255)
                                                                                                                                gCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCTGATCGGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGAAACTCCTTTCCCcggcgg                                                                                                                                                                                                                              <  1:146591/132‑1 (MQ=255)
                                                                                                                                          gcgcTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGAAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATg                                                                                             <  1:60657/251‑1 (MQ=255)
                                                                                                                                                           cTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGAAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCATGCGCTgggg                                                                            >  1:117801/1‑251 (MQ=255)
                                                                                                                                                                                                    gCGGATCTGACCTGGCATCAGAACGAGAGAATTATGTGTAAACCGAAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGATCGGCATCAATGAAGCCCACGAGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGc                                   <  1:141595/251‑1 (MQ=255)
                                                                                                                                                                                                                                   ttATGTTTAAACCGAGACTCCTTTCCNCGGCGGNNACNCTGAAAAAAATGCGTTACGNTTNNNCNNNTNGCTCCNGTNNTGTTTAAACNGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGCATTCAACCACGAAAANTTTCAGATCNNCAGCAATGAAGCCCACTCGCTNGGGNNANNGntttnggn                                                              >  2:48978/1‑189 (MQ=255)
                                                                                                                                                                                                                                     aTGTTTAAACCGAAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCTTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACTAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACTCCAACCTGaaagccgtgaatcgtgtcctgaaacc  <  1:173100/251‑24 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                              
ACGCCACCGCGCAATCTCGTGAAACCGGAATTGCCTCCGTTAAAGCCAATGGCACAAGCCAGACGGTGAAAGACAATACGTAATTACGATGCCGGGCGTGGGTTGTTGGCGTCCGGCGCTTTACCAGAGCCCCCCACAGCGCTACAATGCCCGCCCTTAAAGTGGGGGCACTCCCCTAACCGCTTCATCAGGTGAAGCGGATCTGACCTGTCATCAGAACGAGAGAATTATGTTTAAACCGGAACTCCTTTCCCCGGCGGGAACGCTGAAAAATATGCGTTACGCTTTCGCTTATGGCGCAGATGCTGTTTATGCGGGCCAGCCGCGTTATTCCCTGCGTGTGCGCAACAACGAATTCAACCACGAAAATCTTCAGCTCGGCATCAATGAAGCCCACGCGCTGGGGAAAAAGTTTTATGTCGTGGTCAACATTGCACCGCACAACGCCAAGCTGAAAACCTTTATCCGTGACCTGAAACC  >  NC_000913/2165439‑2165918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: