Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,164,620	G→A	R115R (CGG→CGA)	baeR →	DNA‑binding transcriptional activator BaeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,164,620	0	G	A	100.0%	35.6 / NA	15	R115R (CGG→CGA)	baeR	DNA‑binding transcriptional activator BaeR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

GCGCCAGACACCACCGGATCTGATCCTGTTAGATCTGATGCTCCCTGGCACCGATGGCCTGACGCTGTGCCGGGAAATTCGTCGTTTTTCTGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGGGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAGGCTTCATGGCGCGGTAAAATGCTTGACCTGACGCCTGCGGAATTTCGTCTGCTGAAAACGCTCTCTCACGAACCAGGAAAAGTGTTCTCCCGCGAGCAATTGCTCAATCATCTTTATGACGACTACCGCGTAGTA  >  NC_000913/2164425‑2164863                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    gcgcCAGACACCACCGGATCTGATCCTGTTAGATCTGATGCTCCCTGGCACCGATGGCCTGACGCTGTGCCGGGAAATTCGTCGTTTTTCTGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAgn                                                                                                                                                                                              <  1:39955/251‑2 (MQ=255)    ccAGACACCACCGGATCTGATCCTGTTAGATCTGATGCTCCCTGGCACCGATGGCCTGACGCTGTGCCGGGAAATTCGTCGTTTTTCTGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCATATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTgc                                                                                                                                                                                           >  1:165852/1‑251 (MQ=255)                          cTGTTAGATCTGATGCTCCCTGGCACCGATGGCCTGACGCTGTGCCGGGAAATTCGACGTTTTTATGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGCTGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAACCCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGccc                                                                                                                                                                     >  1:36341/1‑251 (MQ=255)                            gTTAGATCTGATGCTCCCTGGCACCGATGGCCTGACGCTGTGCCGGGAAATTCGTCGTTTTTCTGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAGGTGGTAGCGCGCGTCAAAACCATGTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGCCCg                                                                                                                                                                    <  1:92367/250‑1 (MQ=255)                                naTCTGATGCTCCCTGGCACCGATGGCCTGACGCTGTGCCGGGAAATTCGTCGTTTTTCTGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCCGCGCGAGTTGCAGCAACAGGATGCTGAACGCCCGTTGAt                                                                                                                                                               >  1:127310/2‑251 (MQ=255)                                                 cACCGATGGCCTGACGCTGTGCCGGGAAATTCGGCGTTTTTCTGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGGTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGtt                                                                                                                                              >  1:15672/1‑251 (MQ=255)                                                                     gNCGGGAACTTCGTCGTTTTTCTGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAGGCTTCATGGCGCGGt                                                                                                                          >  1:169150/1‑251 (MQ=255)                                                                                   cgtTTTTCTGACATTCCGATGGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTTCGCCGTTGCAAACCGCAGCGCGAGTTGTAGCAACAGGATGCTGAAAGCTCGTTGAGCATCGACGAAGGTCGGTTTCAGGCTTCATGGCGCGGTAAAATGCTTGAcc                                                                                                             <  1:191755/250‑1 (MQ=255)                                                                                     tttttCTGACAGTCCGATCGTGGTGGGGACGGCAAAAAGCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAGCCATGTTGCGCCGTTGCAAACCGCAGCTCGAGTTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAgg                                                                                                                                        <  1:187113/221‑1 (MQ=255)                                                                                                          gATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAGGCTTCATGGCGCGGTAAAATGCTTGACCTGACGCCTGCGGAATTTCGTctgc                                                                                     <  1:11337/251‑1 (MQ=255)                                                                                                                                  gatcgaacGCCTGCTGGGGCTGGAGATTGGCGGAGATGATTATATCTGTAAGACGTCCAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTg                                                                                                                                                                            >  1:183256/1‑140 (MQ=255)                                                                                                                                             tgctgGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAGGCTTCATGGCGCGGTAAAATGCTTGACCTGACGCCTGCGGAATTTCGTCTGCTGAAAACGCTCTCTCACGAACCAGGAAAAGTGTtc                                                  >  1:5879/1‑251 (MQ=255)                                                                                                                                                       ggAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAGGCTTCATGGCGCGGTAAAATGCTTGACCTGACGCCTGCGGAATTTCGTCTGCTGAAAACGCTCTCTCACGAACCAGGAAAAGTGTTCTCCCGCGAGc                                        >  1:50067/1‑251 (MQ=255)                                                                                                                                                                                 gTAAGCCGTACAGCCCACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAGGCTTCATGGCGCGGTAAAATGCTTGACCTGACGCCTGCGGAATTTCGTCTGCTGAAAACGCTCTCTCACGAACCAGGAAAAGTGTTCTCCCGCGAGCAATTGCTCAATCATCTTTATga                  <  1:115571/247‑1 (MQ=255)                                                                                                                                                                                              cccACGAGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAACCCGCAGCGCGAGTTGCAGCACCAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAGGCTTCATGGCGCGGTAAAATGCTTGACCTGACGCATGCGGAATTTCGTCTGCTGAAAACGCTCTCTCACGAACCAGGAAAAGTGTTCTCCCGCGAGCAATTGCTCAATCATCTTTATGACGACTACCGCgtagta  >  1:105156/1‑250 (MQ=255)                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    GCGCCAGACACCACCGGATCTGATCCTGTTAGATCTGATGCTCCCTGGCACCGATGGCCTGACGCTGTGCCGGGAAATTCGTCGTTTTTCTGACATTCCGATCGTGATGGTGACGGCAAAAATCGAAGAGATCGATCGCCTGCTGGGGCTGGAGATTGGCGCAGATGATTATATCTGTAAGCCGTACAGCCCACGGGAAGTGGTAGCGCGCGTCAAAACCATTTTGCGCCGTTGCAAACCGCAGCGCGAGTTGCAGCAACAGGATGCTGAAAGCCCGTTGATTATCGACGAAGGTCGTTTTCAGGCTTCATGGCGCGGTAAAATGCTTGACCTGACGCCTGCGGAATTTCGTCTGCTGAAAACGCTCTCTCACGAACCAGGAAAAGTGTTCTCCCGCGAGCAATTGCTCAATCATCTTTATGACGACTACCGCGTAGTA  >  NC_000913/2164425‑2164863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑