Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,163,815	G→A	D314N (GAT→AAT)	baeS →	sensor histidine kinase BaeS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,163,815	0	G	A	100.0%	35.6 / NA	14	D314N (GAT→AAT)	baeS	sensor histidine kinase BaeS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

GCACACTGGAGAAAAACCAGCAAATGCGGCGCGATTTTATGGCCGATATTTCTCACGAACTGCGTACGCCATTAGCGGTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCGGGCAGCGCGACAAAAC  >  NC_000913/2163570‑2164029                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                        gCACACTGGAGAAAAACCAGCAAATGCGGCGCGATTTTATGGCCGATATTTCTCACGAACTGCGTACGCCATTAGCGGTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTg                                                                                                                                                                                                                    >  1:5877/1‑251 (MQ=255)                               gcgATTTTATGGCCGATATTTCTCACGAACTGCGTACGCCATTAGCGGTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGGAAGTggcggggggggcc                                                                                                                                                                                      >  1:161102/1‑244 (MQ=255)                                cgATTTTATGGCCGATATTTCTCACGAACTGCGTACGCCATTAGCGGTGCTGAGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGGAAGTGgcgggcggggcg                                                                                                                                                                                      >  1:121470/1‑246 (MQ=255)                                    tttATGGCCGATATTTCTCACGAACTGCGTACGCCATTAGCGGTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAAGTCACGCCGGAGACGGTGGCGTCTTTACAGGCGTAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTGGTCGATGTCTGATGAATGCTCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTc                                                                                                                                                                                  <  1:109683/250‑1 (MQ=255)                                         ggCCGATATTTCTCACGAACTGCGTACGCCATTAGCGGTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGGAAGTGGCGGGGGGGGCATTTTGCG‑aa                                                                                                                                                                            >  1:63733/1‑250 (MQ=255)                                                                                gctgcGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGc                                                                                                                                    <  1:59392/251‑1 (MQ=255)                                                                                          gAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCAAGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCAATCAGTTGTCGATGTCTGCTGAAGGCTCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGGATTTTGCGCAAC‑ATTCGCAAGACGTGGCCTGAAACTGCCATTTTTCCTGCCCGAccatct                                                                                                                          >  1:126957/1‑246 (MQ=255)                                                                                                               ggCGTGCGTAAATTCACGCCGGAGACGGGGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAGCTGGTTGACGATCTCCATCAGGTGTCGAGGTCTGATGAAGGCGCTCACGCCTATCAAATAGCGCCGGTAAATTTGATCCCACGGCTGGAAGTggcg                                                                                                                                                                                               <  1:175122/162‑1 (MQ=255)                                                                                                                      gtAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCCGTCGTGGGCCGAAACTGCAAATTTTCCTGCCAGACAGTATTTCCGTATTTGGCGATAGCGCCCGtttt                                                                                              >  1:71402/1‑250 (MQ=255)                                                                                                                                 ccGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTGGATCCCACTGCTTGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCACTTTTCCCTGCCAGACAGTATCACCGTATTTg                                                                                                               <  1:51015/223‑1 (MQ=255)                                                                                                                                         nnnnnnnnntntANNGGCGNAGGTCGGTACACTGACCAAACTGGNTGACGATCTCCATCAGTTGTCGATGTCTGATNANGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGNAAGTGGCGGGNGGCNNATTTNGNG‑NACGATTCGCCAGTCGTGGCCTGAAACTGCNATTTTCCCTGCCAGACAGTATTANNGTNNNTGNNGATcg                                                                                                        <  2:161102/213‑1 (MQ=255)                                                                                                                                                                                   ggTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCTCTGCTGGAAGTGGCGGGCGGCGCAGTTCGCG‑AACGATTCGTCAGTCGTTGCCTGAAACGGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGcg                                 <  1:217015/251‑2 (MQ=255)                                                                                                                                                                                      tggcGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCGCGCCTATCAAAAAGCAGCGGTAAATTTGATCCCACTGCTGGAAGTGGCGGgcggcg                                                                                                                                                                                        <  1:31169/95‑1 (MQ=255)                                                                                                                                                                                                                  gatgaAGGCGCTCTCGCCTATCAAAAAGCACCGGTAAATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCACTTCCCG‑GCCGATTCCCCAGTTGTGGCCTAAAACTGCAATATTTCCTTCCATACAGTATCAACGTATTTGGGGTTATCGACCGTTTAATTCCGCTCTTCAATAACTTACTGGCAAACAGCCTGCCCTACAATGACAGCGGTGGCAGCCCGCCAAACTCTGCCGGGCCGAGCGACCAAAc  >  1:221256/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                        GCACACTGGAGAAAAACCAGCAAATGCGGCGCGATTTTATGGCCGATATTTCTCACGAACTGCGTACGCCATTAGCGGTGCTGCGCGGTGAACTGGAAGCCATTCAGGATGGCGTGCGTAAATTCACGCCGGAGACGGTGGCGTCTTTACAGGCGGAGGTCGGTACACTGACCAAACTGGTTGACGATCTCCATCAGTTGTCGATGTCTGATGAAGGCGCTCTCGCCTATCAAAAAGCACCGGTAGATTTGATCCCACTGCTGGAAGTGGCGGGCGGCGCATTTCGCG‑AACGATTCGCCAGTCGTGGCCTGAAACTGCAATTTTCCCTGCCAGACAGTATTACCGTATTTGGCGATCGCGACCGTTTAATGCAGTTATTCAATAACTTACTGGAAAACAGCCTGCGCTACACTGACAGCGGCGGCAGCCTGCAAATCTCTGCCGGGCAGCGCGACAAAAC  >  NC_000913/2163570‑2164029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑