Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,158,185	G→A	A975T (GCT→ACT)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,158,185	0	G	A	100.0%	22.5 / NA	10	A975T (GCT→ACT)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (3/7);  total (3/7)

ACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGG  >  NC_000913/2157956‑2158380                                                                                                                                                                                                                                      |                                                                                                                                                                                                    aCCCACCGCAGGTGTTTGCGCACTGCTTGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTGGCTTCTGAGCGCGAGCAAGGCATGTCGCCGCTGGATGCAATCTTCCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGACTCTGCTTTTCGCGCTGCCGc                                                                                                                                                                                <  1:27991/251‑1 (MQ=255)                                  gCTGATTGCTTGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTTGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCTAGGCAATCTACCAGGCTTGTCTGTTGGGTTTTCGTCCTATCCTGATTACCACTCTGGCGACTCGGCTTGGCGCGCGGCCTCTGATTTTGAGTACCGGGGTCGGCGCGGAACTg                                                                                                                                                <  1:192936/250‑1 (MQ=255)                                            ggTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATAGTGAAGAAGAAAGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCGGATCCTGATGACCACTCTGGCGACTCTGc                                                                                                                                                                                               >  1:138574/1‑193 (MQ=255)                                                  gAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGGGAAGAAGAACGCCATCATGATGAACGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGACTCTGCTTGGCGCGCTGCCGCTGATGGTGAGTACCGGGGTCGGCGCGGAACTTCGTCGTCCGTTAGGTAt                                                                                                                               >  1:212620/1‑251 (MQ=255)                                                                                                                                      ctggctgCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGACTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCGGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGAccn                                                 <  1:41185/245‑2 (MQ=255)                                                                                                                                        ggctgCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGACTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGCTTTATTTGCTGTTCGACCGCCTGGCAt                                         >  1:9850/1‑251 (MQ=255)                                                                                                                                                                  cgccgcGCGAGGCAATCTACCAGGCTTGTCTGTTTCTTTTTCGTCCGATCCTGATGACCACTCTGTCGACTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGTCATTGTGGACCAAAATCCGCTTTGCCCGt               <  1:173292/251‑1 (MQ=255)                                                                                                                                                                              cAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGACTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGGATCGGCATTGTCTGCGGTCTGATTGTCAgn                                                                                                   <  1:174802/155‑2 (MQ=255)                                                                                                                                                                               aaTCTACCTGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGACTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGACCTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGaggagg  <  1:92406/251‑1 (MQ=255)                                                                                                                                                                                                 gTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGACTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGATCTGCGTCGTCCGTTAGGTATCgg                                                                                                                            <  1:16588/111‑1 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                    ACCCACCGCAGGGGTTGGCGCACTGCTGGCGTTGCTGATTGCTGGTAGCGAACTGGATGTGATTGCGATTATCGGCATTATTTTGCTGATCGGTATCGTGAAGAAGAACGCCATCATGATGATCGACTTCGCGCTGGCTGCTGAGCGCGAGCAAGGCATGTCGCCGCGCGAGGCAATCTACCAGGCTTGTCTGTTGCGTTTTCGTCCGATCCTGATGACCACTCTGGCGGCTCTGCTTGGCGCGCTGCCGCTGATGTTGAGTACCGGGGTCGGCGCGGAACTGCGTCGTCCGTTAGGTATCGGCATGGTCGGCGGTCTGATTGTCAGCCAGGTGCTGACGCTGTTTACCACGCCGGTGATTTATTTGCTGTTCGACCGCCTGGCATTGTGGACCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGG  >  NC_000913/2157956‑2158380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑