Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,153,068	G→A	P21S (CCC→TCC)	yegL ←	IPR002035 domain‑containing protein YegL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,153,068	0	G	A	100.0%	22.5 / NA	9	P21S (CCC→TCC)	yegL	IPR002035 domain‑containing protein YegL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/7);  total (2/7)

ACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCAATAATGAAATAAGAGCAAACGAAGAAACTTTCAGAATTAGGCCCGGCGGCGCAGAAAATTTTGCTATTCGC  >  NC_000913/2152864‑2153250                                                                                                                                                                                                             |                                                                                                                                                                                       aCAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCAGCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGAGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGtn                                                                                                                                          <  1:53952/251‑2 (MQ=255)       tGGGCGGAAAAAAATTTGCGGCGCGGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAGCCAGAAACATCCAGCAGCAAAATGCAGGAGCAACGTGGTTCCGGGTTACTGGCAAAATCGCtggtg                                                                                                                                                   <  1:151197/236‑1 (MQ=255)                                       ggTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAAGCCGGCGTTAAGTTCATTAATAGGTCGACCGTTCATAGAACCAGAAACATCCAGCATCAAAATGCATGAGCAACGTGGTTCCTGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTc                                                                                                                                  <  1:209270/221‑1 (MQ=255)                                               cacaTGCACCGGGACGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGAGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTc                                                                                            <  1:141462/251‑1 (MQ=255)                                                                      caATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTTCCGTTCATAGAACCAGAAACATCCAGCATCAAAATGCAGGAGCAACGTGGTTCCGGGTTTCTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCGCATAAGAAATCCTTTCTTaga                                                                                                          <  1:84675/214‑1 (MQ=255)                                                                                   cACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTACCCAATCCGGCGTCAAGTTCATTAATAGGTCTCCCCTTCCTCGAACCCGAAAAATCCAGCAGCAAAATCCAGGAGCAACGTGGTTCCGGGTAACTGGCAAAATCGCTGGTGGCAACCGTGATTTGTTCGCTCATACGAAATCCTTTCTTAGAGAATTCTCGAGGTCCGGTATCATACCgcc                                                                             >  1:199492/1‑228 (MQ=255)                                                                                                       gATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCCTTAATAGGACTACAGTACATAGAACAAGAAACATCCAGCAGCAAAATGCAGGAGCAACGTGGTTCCGGGTTACTGGCAAAATCGATGGTGGCAAACGTGATTTGTTCGCTCATACGAAATACTTTCTTAGAGAATTATCGAGTTCCGGTATCATACCGACTGCAATAATGAAATAAGAGCAAACGAAGAAACTTTCAGAAt                                    >  1:191337/1‑251 (MQ=255)                                                                                                            gcaagcaGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGAGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCAATAATGAAATAAGAGCAAACGAAGAAACTTTCAGAATTAGGc                               <  1:49566/251‑1 (MQ=255)                                                                                                                                          ccGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGAGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCAATAATGAAATAAGAGCAAACGAAGAAACTTTCAGAATTAGGCCCGGCGGCGCAGAAAATTTTGCTATTCgn  <  1:54672/250‑2 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                       ACAGGATGGGCGGAAAAAAATTAGCGGCGCTGGTAAAGGGTTGTTCCACATGCACCGGGCCGAACGTCACAATGCCGAGTTCCACTCTTTTTAAAGCCAGCGGATCAGCAAGCAGTTCATCACGAAAGGTAACCAATCCGGCGTTAAGTTCATTAATAGGTCTACCGTTCATAGAACCAGAAACATCCAGCAGCAAAATGCAGGGGCAACGTGGTTCCGGGTTACTGGCAAAATCGCTGGTGGCAAACGTGATTTGTTCGCTCATAAGAAATCCTTTCTTAGAGAATTATCGAGGTCCGGTATCATACCGACTGCAATAATGAAATAAGAGCAAACGAAGAAACTTTCAGAATTAGGCCCGGCGGCGCAGAAAATTTTGCTATTCGC  >  NC_000913/2152864‑2153250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑