Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,150,076	G→A	Q304* (CAA→TAA)	yegI ←	putative serine/threonine‑protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,150,076	0	G	A	100.0%	28.8 / NA	12	Q304* (CAA→TAA)	yegI	putative serine/threonine‑protein kinase YegI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

GGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGG  >  NC_000913/2149854‑2150264                                                                                                                                                                                                                               |                                                                                                                                                                                             ggAAATGATCGGGTAATGGCATTTGCAAGGCGGGCGGTGCTACTGACGCCATCCCCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCAGTGCTGAAACGATACATTTCTTTAACTGTTAGCGTAGAGATGCCAGTGCCGCTACCCan                                                                                                                                                                  <  1:174990/251‑2 (MQ=255)  gAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACCAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCCGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTAGCGTCGAGAGTCCAGTGCCGCTACCCACg                                                                                                                                                                 >  1:80084/1‑251 (MQ=255)          cGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGCCGCCATCTCCATCCCCCCGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTAGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGc                                                                                                                                                          <  1:18382/250‑1 (MQ=255)                          aaaTGCTGGCGGTGCTACTGACCCCACCACCATCGCCCAGACTTCCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTAGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTg                                                                                                                                         <  1:76754/250‑1 (MQ=255)                                     ntGCTACTGACGCCATCACCATCGCCCAGACTTTCGCAAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTAGCGTAGAGAGTCCAGTGCCACTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACtt                                                                                                                              >  1:25131/2‑251 (MQ=255)                                                            gCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCAGAAACGATACATTTCTTTAACTGTTAGCGTAGAGAGTCCAATGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACAt                                                                                                       >  1:195881/1‑251 (MQ=255)                                                                            tcacaAAATTCCCGCCGGTGGTAATGACCTCTTCGCAGAGGTCAATAAAATAGATAAAACGTTGCTTATCGAGGGCACACGCCGGGAAGTCAGTAAAATGAACGGGGTCAACATGCGTTGCTGAAAAGATACATTTCTTTTACTGTTAGAGTCGAGAGTCCAg                                                                                                                                                                               >  1:104908/2‑163 (MQ=255)                                                                                                                                                                 gCAGTCAGTCAAAGGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTAGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATGATCTGACGCGTAGGCATAACGGAAATggn  <  1:52909/251‑2 (MQ=255)                                                                                                                                                                   agtcagtcAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTAGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCg                                                                                                                                             >  1:96184/1‑110 (MQ=255)                                                                                                                                                                      cagtcaCATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTAGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGgcggcg                                                     >  1:50160/1‑195 (MQ=255)                                                                                                                                                                        ntcnAATNANNGNNGTANACATGCATTGCTGAAACGATCNNTTTCTTGAANTGTTAGCGTAGAGAGGCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCNGTTGCCACGNNACNGGCCTNGNNNGNNNGCNGAAACATGGCTTCAACATCNCTNNGTAACANCGATAGCGGAATAGATCGTGgcggcg                                                     <  2:50160/192‑1 (MQ=255)                                                                                                                                                                                       tAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTAGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGt                                                   <  1:186858/180‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                             GGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTACCCACGCTTTAGCCGTCGGACGCCCGGTTGCCACGCCACTTTCCGTGAATGCCTGCTGAAACATGGCTTCAACATCACTCGGTAACATCGATAGCGGAATAGATCGTGGCGGCGGTTTTAAACCACGTCGCTGATTATCTGACGCGTAGGCATAACGGAAATGGG  >  NC_000913/2149854‑2150264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑