Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,145,515 G→A M750I (ATG→ATA)  dgcE → putative diguanylate cyclase DgcE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,145,5150GA90.0% 18.8 / ‑6.2 10M750I (ATG→ATA) dgcEputative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (4/5);  minor base T (0/1);  total (4/6)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGTGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATC  >  NC_000913/2145277‑2145746
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       
cACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTTCCATTATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATACTGCGTTCCAg                                                                                                                                                                                                                              <  1:189373/250‑1 (MQ=255)
    cAAAATGCTGCGCCAGCTGAGCTACAGCGCCTACCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGCGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGAGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATACTGCGTTCCAGCGa                                                                                                                                                                                                                           >  1:183822/1‑249 (MQ=255)
                                              aCTGACGCATCTCGCCAACCGCGCCAGTTTTGAGGAACAACCGCGTATCCTGCTGCAAAAGGTAAACAGTACAAATCAGCGACATGCCCTGGTGTTTATCGATCATGATCGCTTTAAAGCGGAGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTCTACTGCGTTCCAGCGACGTGCTGGCGCGCCTCGGTGGGGATGACTTGTGTCTGGTGCTAc                                                                                                                                                                               >  1:88943/1‑251 (MQ=255)
                                                                                     acTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCTCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGGCGTTAATGCTGAGTATTCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTacac                                                                                                                                        <  1:64751/251‑1 (MQ=255)
                                                                                                      aaaCGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGTGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATACTGCTTTCCAGCGACGTGCTGGCGCTACTCGGTGGGGATGAATTTGTTCTGCTGCTACCAGACTGTAATGTTGAAATCGCGCTTTTTATCGCTACACGCATTATCAGTGCCGTg                                                                                                                       <  1:176431/251‑1 (MQ=255)
                                                                                                                                      cTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATACTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTc                                                                                                                                                                                        <  1:145757/154‑1 (MQ=255)
                                                                                                                                                                         gAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGATGGGTATACTGCGTTCCAGCGACGTGCTGGCCCGAATCGGGGGGGATGAATTTGGTCTGGTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTTTCGCTACACGCATTATCAGTGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGGGCATCGGGTAGGTGCCAGTGCCGGGATTAACttg                                                     >  1:97084/1‑250 (MQ=255)
                                                                                                                                                                                                  ggTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATACTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTgn                                                                                                                                                                                          <  1:62258/92‑2 (MQ=255)
                                                                                                                                                                                                                   cgAACTGGCGGCGTTAATGCTGAGTATACTGCGTTCCAGCGACGGGCTGGCTCGACTCGGTGGGGATGTATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAgc                                                                                                                                                           <  1:85719/106‑1 (MQ=255)
                                                                                                                                                                                                                           cgtcgtTAATGCTGAGTATACTGCGTTCCAGAGACGTGCTGGCGCGACTCGGTGGGGCTGACTTTGGTCTGCTGCTACCCGACTGTAATGTTGCAAGCGCGCGTTTTATCGCTACACGCATTATCAGTGCCGTGCATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGGGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATc  >  1:189212/1‑251 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       
CACGCAAAATGCTGCGCCAGCTGAGCTACAGCGCCTCCCATGATGCACTGACGCATCTCGCCAACCGCGCCAGTTTTGAGAAACAACTGCGTATCCTGCTGCAAACGGTAAACAGTACACATCAGCGACATGCCCTGGTGTTTATCGATCTTGATCGCTTTAAAGCGGTGAATGACAGCGCCGGGCATGCGGCGGGTGACGCTTTGCTGCGCGAACTGGCGTCGTTAATGCTGAGTATGCTGCGTTCCAGCGACGTGCTGGCGCGACTCGGTGGGGATGAATTTGGTCTGCTGCTACCAGACTGTAATGTTGAAAGCGCGCGTTTTATCGCTACACGCATTATCAGTGCCGTGAATGACTATCACTTTATATGGGAAGGCCGTGTGCATCGGGTAGGTGCCAGTGCCGGGATTACCTTGATTGATGACAACAATCATCAGGCGGCAGAAGTGATGTCGCAGGCTGATATC  >  NC_000913/2145277‑2145746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑