Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,144,574	G→A	A437T (GCG→ACG)	dgcE →	putative diguanylate cyclase DgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,144,574	0	G	A	100.0%	21.5 / NA	9	A437T (GCG→ACG)	dgcE	putative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/4);  total (5/4)

GATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTAC  >  NC_000913/2144363‑2144788                                                                                                                                                                                                                    |                                                                                                                                                                                                                       gATAAGCGGTGAAATAAACTCCTATTCAATGAAAAAACGCTCCTACAACCGCAATGGCGATGTTGTCTGGTCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGTCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTTACGAAACGGTCGGGATTGGCATCtgggag                                                                                                                                                                                                 <  1:66358/235‑1 (MQ=255)                                                                       gCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAACAGGCGGGCTTGGAATCTGGGAGGGGGAGTTGGACCCGAATTTTTTTTGCTGGGATAAGCGGATTTTCGAACTGTAATAAATTCCCCCACATATCAAAccccactcgc                                                                                                            >  1:193759/1‑242 (MQ=255)                                                                            gcttgcCGTCTCACTGGTGCGCCACACGGATGGCTCGCCGCTCTATTTTATCGCTCAGAGTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAATTGAGGGAGCGCATCACTCTGGCTAACGAAACGGGCGGGATTGGCATCtgggagtgg                                                                                                                                                                                              <  1:138557/163‑1 (MQ=255)                                                                             cttgcCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTTTCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCACCAACTGATGGAGCGCATCACTCTGGCTAACGAAACGGGCGGGATTGGCATCTGGGAGCGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGtgtg                                                                                                     >  1:87267/1‑251 (MQ=255)                                                                                   gTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCCCCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAACGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACg                                                                                                >  1:208173/1‑250 (MQ=255)                                                                                           ggTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAACGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAgtgcgtgc                                                                                       <  1:47867/251‑1 (MQ=255)                                                                                                                                                   aCGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAACGGGCGGGATTGGCATCTGGGAGGGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACgn                               <  1:55590/251‑2 (MQ=255)                                                                                                                                                        cTAAAACGCACCGAACAGGTCCATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAACGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACAAATCAAACCGAACTGGCAGGTGTGGTACGAg                                                                                              >  1:58905/1‑183 (MQ=255)                                                                                                                                                                                cAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAACGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTAc  >  1:59931/1‑251 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                       GATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAGTGGGAGTTGAAGCCGAATATTTTTAGCTGGGATAAGCGGATGTTCGAGCTGTATGAAATTCCTCCACATATCAAACCGAACTGGCAGGTGTGGTACGAGTGCGTGCTGCCGGAAGATCGCCAGCATGCCGAAAAAGTGATTCGTGATTCGTTGCAATCACGCTCGCCCTTTAAACTGGAATTTCGCATTAC  >  NC_000913/2144363‑2144788

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑