Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,144,394	G→A	E377K (GAA→AAA)	dgcE →	putative diguanylate cyclase DgcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,144,394	0	G	A	100.0%	26.0 / NA	10	E377K (GAA→AAA)	dgcE	putative diguanylate cyclase DgcE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

GCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAG  >  NC_000913/2144164‑2144597                                                                                                                                                                                                                                       |                                                                                                                                                                                                            gCGAAACCCACTTTCGGAACGCGCTGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGCCAATGGCTGCAAACCAACAACGCGCTCAGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGAACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACATATTCAATGAAAAAACGatactaaaaacgc                                                                                                                                                                                         >  1:150378/1‑244 (MQ=255)                             atatTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGAAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGttgcttn                                                                                                                                                             <  1:41542/251‑2 (MQ=255)                                  ccGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGAAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGtc                                                                                                                                                        <  1:79670/251‑1 (MQ=255)                                                        gggCACCGAGGGAAAATGGCGGCAAACCAACAAAGCGTGCTGGCATTTTCTCGGGTACAGTCAGGAAGGGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCACAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGAAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATg                                                                                                                                  <  1:197591/251‑1 (MQ=255)                                                                                   caacaaAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCACTAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACCCCTATTCAATGATAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTc                                                                                                          >  1:1583/1‑248 (MQ=255)                                                                                                  cagacgggggcgcttccgagctgAAGAGCTGCTCGGACTCACCTGTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCGATGCAAGGAAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGGTGCGTGCCGTCTCACTGGTACGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACg                                                                                        <  1:28831/229‑1 (MQ=255)                                                                                                         ctcGGGTACAGTCAGGAAGAGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGAAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTaa                                                                                  >  1:99813/1‑250 (MQ=255)                                                                                                                                                                     gaTCGCAATAAAGATCTCCAACAGGTTGAAAAGCTTATAAGCGGGGAAATAAACACCTATGCAATGAAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGcc                                                                                                                                                           <  1:178026/117‑1 (MQ=255)                                                                                                                                                                             tAAAGATCTCCAACAGGTTGAAAAGCNGATAAGNNGTNAAATAAACACCTATTCAATNAANNNANNNTNCTACANCCNNAATGGCGATNTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGNTCTATTTTANNGCTCAGATTGAAGACATTAACNAGCNNANNCNCACNGan                                                                       >  2:47867/1‑192 (MQ=255)                                                                                                                                                                                                        gATAAGCGGTGAAATAAACTCCTATTCAATGAAAAAACGCTCCTACAACCGCAATGGCGATGTTGTCTGGTCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGTCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTTACGAAACGGTCGGGATTGGCATCtgggag  <  1:66358/235‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                            GCGAAACCCACTTTCGGAACGCGATGGAATATTCCGCTATCGGTATGGCGTTAGTGGGCACCGAGGGACAATGGCTGCAAACCAACAAAGCGCTCTGCCAGTTTCTCGGGTACAGTCAGGAAGAGCTGCGCGGACTCACCTTTCAGCAACTGACCTGGCCGGAGGATCTCAATAAAGATCTCCAACAGGTTGAAAAGCTGATAAGCGGTGAAATAAACACCTATTCAATGGAAAAACGCTACTACAACCGCAATGGCGATGTTGTCTGGGCGTTGCTTGCCGTCTCACTGGTGCGCCACACGGATGGCACGCCGCTCTATTTTATCGCTCAGATTGAAGACATTAACGAGCTAAAACGCACCGAACAGGTCAATCAGCAACTGATGGAGCGCATCACTCTGGCTAACGAAGCGGGCGGGATTGGCATCTGGGAG  >  NC_000913/2144164‑2144597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑