Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,134,549	G→A	T368T (ACC→ACT)	wzc ←	protein‑tyrosine kinase Wzc

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,134,549	0	G	A	100.0%	25.6 / NA	10	T368T (ACC→ACT)	wzc	protein‑tyrosine kinase Wzc
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/5);  total (5/5)

GGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAAC  >  NC_000913/2134347‑2134755                                                                                                                                                                                                           |                                                                                                                                                                                                               ggCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGAGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCt                                                                                                                                                                >  1:126067/1‑251 (MQ=255)      aGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGAGTTTTCGGCATCGCCTTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCan                                                                                                                                                           <  1:19825/251‑2 (MQ=255)        gATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCCGCTGTCCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGAGTTTTCGGCATCGCCTTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAgcn                                                                                                                                                         <  1:38670/251‑2 (MQ=255)                               gCCCTTTATTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGAGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTg                                                                                                                                  >  1:166393/1‑251 (MQ=255)                                                            tGAGTGATTGCCTGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGCGATTTTAAGCTACTGCTCTTTATTCAGGAGTTGCCTATAGACCTGCTGACCCGACTCGACATGGCGGGGCAGACGGACAATCTCCTGCTGAGTTTTCGGCATCGCCGTTACGCGACCGTTCAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTTTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTAc                                                                                                       >  1:117266/1‑249 (MQ=255)                                                                      ccGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGAGTTTTCgg                                                                                                                                                                                                        <  1:139172/142‑1 (MQ=255)                                                                      ccGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCACCTGCTGAGTTTTCGGCATCGCCGTTACGCGACCGATAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGAtt                                                                                           >  1:29756/1‑251 (MQ=255)                                                                                            gCCGACGGTGCTGGCCTCGGTGATTTTCATCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGAGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCg                                                                                                                                                                   <  1:83141/157‑1 (MQ=255)                                                                                            gCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGAGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTgn                                                                                                            <  1:26113/212‑2 (MQ=255)                                                                                                                                                                gACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGAGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAAc  >  1:68249/1‑250 (MQ=255)                                                                                                                                                                                                           |                                                                                                                                                                                                               GGCCAAGGATAATCGCGCCGAGGATAATCAGCCCTTTCTTCGGTTTCAGCACGCCAGGCTGAGTGATTGCCGGGTCAACAATGCGCACATCGCCGACGGTGCTGGCCTCGGTGATTTTCAGCTCCTGCTCTTTATTCAGCAGTTGCATATAGACCTGCTGACCAGACTCGACATCGCGGGTCAGACGGACAATCTCCTGCTGGGTTTTCGGCATCGCCGTTACGCGACCGTTAAGTTTGGCTTTTTCGTCTTCCAGCGCCTGACGTTTCTCCAGCAGCGTGCGGTACGCCGGGTGAACTTTGGTGTACAGCTTGGAGATTTCCGCCTCTTTAAAGGTCAGTTCGTTCAACTGCGCATCGATGTTCACCATCGAATCGAGCACCGCTTTTGCTTCCAGCGGCAGATCAAC  >  NC_000913/2134347‑2134755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑