Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,126,648	G→A	I181I (ATC→ATT)	fcl ←	GDP‑L‑fucose synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,126,648	0	G	A	100.0%	13.0 / NA	7	I181I (ATC→ATT)	fcl	GDP‑L‑fucose synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/2);  total (5/2)

CCACTTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATCGCGTCCGTACTGGCGGTTGTATGATTCGCACAGTTTGATCC  >  NC_000913/2126401‑2126763                                                                                                                                                                                                                                                        |                                                                                                                    ccacTTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGGCATGAATGCTCGCCGCCGCCATATCATCTACTTGCAGAAAGTCTCGCAGCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGAATc                                                                                                                  <  1:190086/251‑1 (MQ=255)         gCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCACGTGCCGACGTTAATGTGCGACAAAATCGGCTGGGTGTTCTCCAGACAGACTTCATGCGCCAGATCCATGACATGACTGCTCGCCGCCGCCCTATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGAATCACATGCg                                                                                                           >  1:103899/1‑250 (MQ=255)                   gcgcCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGAATTCATGCGCCAGCTACATGACATGAATGCTCGCCGCCGCCGTATCATCGACGTGCAGAAATTCGCGCATCGGTGTATCGCTGACCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCCGCAATGCTGGAATCACATGCGCCTTACTCgtg                                                                                                >  1:210494/1‑249 (MQ=255)                                                                          caTCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGACAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGAATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATc                                           >  1:183310/1‑249 (MQ=255)                                                                                   ggTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGAATCACATGCGAATTAc                                                                                                     >  1:66043/1‑182 (MQ=255)                                                                                             agccagACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGAATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATCGCGTCCGTACTGGCGGTTGt                       >  1:68042/1‑250 (MQ=255)                                                                                                                   ccATGACATGAATGCTCGCCGCCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGAATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATCGCTTCCGTACTGGCGGTTGTATGATTCGTACAGTTTGATcc  <  1:195051/249‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                    CCACTTTGGCGATGGTTTGCGCCAGCTCGCGGATAGTGCAGTCAACGCCCGTGCCGACGTTAATGTGCGACAACATCGGCTGGGTGTTCTCCAGCCAGACTTCATGCGCCAGCTCCATGACATGAATGCTCGCCGCCGCCATATCATCGACGTGCAGAAATTCGCGCATCGGTGTACCGCTGCCCCATACCACCACGTCCGGCGCATTCTGTGCCGTCGCCTCGTGGAAGCGACGCAGCAATGCTGGGATCACATGCGAATTACTCGGGTGGAAGTTGTCGTGTGGCCCGTACAGGTTGGTCGGCATGACTGAGCGGTAATCGCGTCCGTACTGGCGGTTGTATGATTCGCACAGTTTGATCC  >  NC_000913/2126401‑2126763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑