Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,123,372	G→A	D383D (GAC→GAT)	cpsB ←	mannose‑1‑phosphate guanylyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,123,372	0	G	A	91.7%	27.8 / NA	12	D383D (GAC→GAT)	cpsB	mannose‑1‑phosphate guanylyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (6/5);  minor base C (1/0);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGGTCGCCCGCGTCGATAGAGTCATATTTGCCCCACGGACGATACACTTCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATC  >  NC_000913/2123154‑2123612                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 tCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGATCGCCCGCGTCGATAGAGTCATATTTGcccc                                                                                                                                                                                                                   <  1:67001/250‑1 (MQ=255) tCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACGCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGATCGCCCGCGTCGATAGAGTCATATTTGcac                                                                                                                                                                                                                    <  1:202098/249‑3 (MQ=255)               cGGAATTTTCCCCGGGGTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGCTCGCCCGCGTCGATAGAGTCATAttt                                                                                                                                                                                                                        >  1:10609/1‑231 (MQ=255)                        ncccGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGATCGCCCGCGTCGATAGAGTCATATTTGCCCCACGGACGATACACTTCGCGATGc                                                                                                                                                                                            >  1:132297/2‑250 (MQ=255)                                                     ccAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGCCTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGCTGCGTTTCACCTGGTAGCGATCGCCCGCGTCGATAGAGTCATATTTGCCCCCCGGACGATACACTTCGCGATGCACCCGATGCTCATGGCGCCCATCGGCTTTg                                                                                                                                                              >  1:152085/1‑251 (MQ=255)                                                        gCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGATCGCCCGCGTCGATAGAGTCATATTTGCCCCACGGACGATACACTTCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATc                                                                                                                                                           <  1:122134/251‑1 (MQ=255)                                                          ggAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCGCCATCAATGGTGACTTTTGCCGTTCTCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCCCGCCCTGTTTCTCGGTGATGCGTTTCACCTGGTAGCGATCGTCCGTGTCGAGAGAGTCATATTTTTCCCACGCACGATACACTTCGCGAGGCACCCGATGGTCATGGCGACCATCGGCTTTGATCTg                                                                                                                                                         <  1:92059/251‑1 (MQ=255)                                                                                                  cACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGATCGCCCGCGTCGAGAGAGTCATATTTGcccc                                                                                                                                                                                                                   <  1:167041/153‑1 (MQ=255)                                                                                                                                                                         aCCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGATCGCCCGCGTAGATAGAGTCATATTTGCCCCACGGAAGATACACTTCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCATATACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCCACGGTGGTGACCAGGcc                                             >  1:145993/1‑248 (MQ=255)                                                                                                                                                                          ccGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGATCGCCCGCGTCGATAGAGTCATATTTGCCCCACGGACGATACACTTCGCGATGCACCCGCTGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGcc                                             >  1:119846/1‑247 (MQ=255)                                                                                                                                                                                                                 ccTGGTAGCGATCGCCCGCGTCGATAGAGTCATATTTGCCCCACGGACGATACACTTCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATc  >  1:122213/1‑251 (MQ=255)                                                                                                                                                                                                                    ggTAGCGATCGCCCGCGTCGATAGAGTCATATTTGCCCCACGGACGATACACTTCGCGCCGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTT‑CACATcc                                                                                                                                   >  1:86523/1‑118 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                 TCAATTAAATCGAGCGGAATTTTCCCCGGGTTTTCCAGGCAATGCGTCGCCCCCAGCGGAATATAAATGGACTCGTTTTCACCAAGCAGTTTGATATCACCATCAATGGTGACTTTTGCCGTTCCCGCGACAACCACCCAGTGTTCCGCGCGGTGATGGTGCATCTGTACCGACAAGCCCTCGCCCGGTTTCACGGTGATGCGTTTCACCTGGTAGCGGTCGCCCGCGTCGATAGAGTCATATTTGCCCCACGGACGATACACTTCGCGATGCACCCGATGCTCATGGCGACCATCGGCTTTGATCTGCTCGACCACTTTTTTCACATCCTGTACCGCGTTACGGTCGGCAATCAGCACCGCATCTTTGGTCTGCACCACTACCAGATCTTTCACCCCGACGGTGGTGACCAGGCCAGATTCAGCATACACATAGCTGTTTTCAGTTTTGTGATTAATC  >  NC_000913/2123154‑2123612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑