Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,120,462	G→A	P365S (CCG→TCG)	wcaJ ←	UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,120,462	0	G	A	100.0%	24.4 / NA	11	P365S (CCG→TCG)	wcaJ	UDP‑glucose:undecaprenyl‑phosphate glucose‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/4);  total (7/4)

ACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTG‑TTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTGATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGG  >  NC_000913/2120222‑2120685                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                aCCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTG‑TTCGTTATGCGCTACTGCGTGCGTACGTGGACTGACAATCGACATCCCCCCGGTCAGCACATCGATAAACTGCGACAATTCATcc                                                                                                                                                                                                                       <  1:103974/251‑1 (MQ=255)                                         gACGCGCTTTTCCCTTTTCTCCAGCGTGACCGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAACGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTGCTTC‑CTACGCCCTACTGCGCGCGGCCGTGGCCCGACAATCGACCTCCCCCCGGTCAGCAAATTGATAACCTGCGACAACCTATCCAGCGCCGTACGGCGCAGCAAGTTCCTCACTTTGGTGActc                                                                                                                                                                               >  1:45452/1‑249 (MQ=255)                                                                                        cGTTTATCTGCGCCCAGCCTGTAATGCCCGGTTTCACCTTATGTCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTG‑TTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGTCAGCACATTGATAAACTGCGACAATTCATCCAGCGAGGGACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCTTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCAtn                                                                                                                                <  1:42885/251‑2 (MQ=255)                                                                                                                           aCCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTG‑TTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGTCAGCACATTGATAAACTGCGACAATTCATCCAGCGNgggnnnnngcnn                                                                                                                                                                                                      >  2:136052/1‑136 (MQ=255)                                                                                                                           aCCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTG‑TTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGTCAGCACATTGATAAACTGCGACAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGtn                                                                                                                                       <  1:136052/209‑2 (MQ=255)                                                                                                                           aCCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGAGACTG‑TTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGTCAGCACATTGATAAACTGCGACAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCAGCACTTTCATGGAACGGAACTTCCACACGTTGATCgg                                                                                             <  1:184572/251‑1 (MQ=255)                                                                                                                                                 nttCAATGAGCTGTCGATACTG‑TTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGTCAGCACATTGATAAACTGCGACAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTGATCGGCTTGCCATCCATGCCGTAGCg                                                                        >  1:133635/2‑250 (MQ=255)                                                                                                                                                           cTGTCGATACTG‑TTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGCCATCCCCCCGGTCAGCACATTGATAAACTGCGACAATTCATCCAGCGCGGTACGGCGCAGAAAGTTCCCCCCTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCCCTTTGTCGTTCTCCATCACTTTCATggaacgga                                                                                                                >  1:99457/1‑200 (MQ=255)                                                                                                                                                                                             cgGACGTGGACCGACAATCGACATCCCCCCCATCAGCACATTGATAAACTGCGACAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGAGGATCGTTCTGCGTCGCCTggg                                                                                                                                                       >  1:79352/1‑128 (MQ=255)                                                                                                                                                                                                                gNCATCCCCCCGGTCAGCACATTGATAAACTGCGACAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTGATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATacagcaca          >  1:168609/1‑250 (MQ=255)                                                                                                                                                                                                                       ccccGGTCAGCACATTGATAAACTGCGACAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGCCGAGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTGATCGGCTTGCCATCCATGCCGTAGCGCGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCggg  >  1:210293/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                ACCAGACGCTCCATTCGCGGATGTACTCAAGGTCGAACTCGACGCGTTTTTCCATTTTCTCCAGCGTGTCGGTTTCGCCGCGCCAGCCGTTAATCTGCGCCCAGCCGGTAATGCCCGGTTTCACCTTATGGCGCAGCATGTAGCCTTCAATGAGCTGTCGATACTG‑TTCGTTATGCGCTACTGCGTGCGGACGTGGACCGACAATCGACATCCCCCCGGTCAGCACATTGATAAACTGCGGCAATTCATCCAGCGAGGTACGGCGCAGAAAGTTCCCCACTTTGGTGACGCGCGGATCGTTCTGCGTCGCCTGGGTCACCACTTTGTCGTTCTCCATCACTTTCATGGAACGGAACTTCCACACTTTGATCGGCTTGCCATCCATGCCGTAGCGAGTCTGGCGGAAAATAACCGGCCCTGGTGAACTGAGTTTCACCGCCAGCGCAATACAGCACAGCACCGGG  >  NC_000913/2120222‑2120685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑