Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,032,423 G→A V219V (GTC→GTT)  yedJ ← putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,032,4230GA100.0% 19.5 / NA 10V219V (GTC→GTT) yedJputative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

CTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAAC  >  NC_000913/2032181‑2032653
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      
cTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACAAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGACATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTc                                                                                                                                                                                                                                >  1:88488/1‑251 (MQ=255)
                                                                 cagTTTCTCCAGCATTGCCTGGGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCAGCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTTTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGtgct                                                                                                                                                               <  1:81731/251‑1 (MQ=255)
                                                                          cAGCATTCCCTATGCGGCTTTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAATCTCGACTAAAAAATGCGCGTTGTGCTGCGCCAgct                                                                                                                                                      <  1:11869/251‑1 (MQ=255)
                                                                                 gCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATgcg                                                                                                                                                                        >  1:50187/1‑226 (MQ=255)
                                                                                       gCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGc                                                                                                                                         <  1:177680/251‑1 (MQ=255)
                                                                                                                  ntancTGNTNNTNNTTCNTGAATAGATTTCACCGGCCACNNGAGATGGCCNACAGTTTAACATAATTCAATCTATTTAGGGCCACGCGCTCAGCCAGCGGATGANAACGCATCTNNCANCTTGGNANNNANNNCTNCATCCTCCCCCGCCAGTTCNGCNNTGAGCTNCGCCATAAACTCGACTAAAAAATgcg                                                                                                                                                                        <  2:50187/189‑1 (MQ=255)
                                                                                                                                                                  ccGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAAGGCATCTATCACCTTGTGATCAACAACTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAACCTCgcc                                                                                                                                                                                   >  1:123779/1‑132 (MQ=255)
                                                                                                                                                                         nttAACATAATTACAGCCATTTAGGGCCACGCACTCACCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCCTCATTCTACCCCGCCAGTTCGGc                                                                                                                                                                                                           >  1:23236/2‑103 (MQ=255)
                                                                                                                                                                              cATAATTACAGCCATTTAGGGCCACGCGCTCAGCAAGCGGATGAAAACGCATCTATCCCCTTGTGATCACCACCTTCATTCTCCCCCGCCAGTTCGGCACTGCGCTTCGCCATAAACTCg                                                                                                                                                                                     >  1:93154/1‑120 (MQ=255)
                                                                                                                                                                                                      ngcgcTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAAc                            >  1:127421/2‑249 (MQ=255)
                                                                                                                                                                                                                                         cTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGAAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAAc  <  1:76307/240‑1 (MQ=255)
                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                      
CTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTTACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCACGCGCTCAGCCAGCGGATGAAAACGCATCTATCACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCAAACAGCGCCACGCCCAATGCCCCTGAAAC  >  NC_000913/2032181‑2032653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑