Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,003,264	C→T	E115K (GAA→AAA)	fliC ←	flagellar filament structural protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,003,264	0	C	T	100.0%	36.5 / NA	14	E115K (GAA→AAA)	fliC	flagellar filament structural protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/6);  total (8/6)

TGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTT  >  NC_000913/2003018‑2003492                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     tgtttgtGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTATTGGTAACTTTATCTTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCATTCTGCTTCAGATCGATAGTTATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATGTCATCCAGACGTGATTTAATTTTGTc                                                                                                                                                                                                                                   <  1:51217/250‑1 (MQ=255)       tggtggTAGCAACAAAAGCAGTTACTGGAGCAATAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGACAAGAGTTTTAGCATCAATCTGCTTCAGATCGACAGTGATAGTCTGGTTATAATTTGCGCCACCCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGCCCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTACTGGAt                                                                                                                                                                                                                             >  1:112975/1‑250 (MQ=255)                                    gCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACAATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTAcc                                                                                                                                                                                               >  1:161214/1‑251 (MQ=255)                                          gTGGTAACTGTATAGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACACAGAGTTAGTACCGGTAGt                                                                                                                                                                                         >  1:167537/1‑251 (MQ=255)                                                                                                                     atagtgatagtCTGGTTATCATTTGCGCCAGCCTGGATTTTCATGGAGCCATTTGTTGCCGGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACTGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGGATTAGTCTGTACCGTCAGTGCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTgcgc                                                                                                              <  1:204011/251‑1 (MQ=255)                                                                                                                                               gcCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGCCTGACCAGATACGCGGTCACTTTCATCCAGACGGGCTTTAATTTTGTCATGGATAGTAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCCCACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGcc                                                                                                                             >  1:211795/1‑210 (MQ=255)                                                                                                                                                              cATGGAGCCATTTTTTGCCAGCACGTTCACGCCTTTGAAATGGGTCTGACCAGCTACGCGGTCAATTTAATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGATGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGAAACGGAGATAACGTCGGTGGAGTTACGGGCCTCCTGAGta                                                                      >  1:141354/1‑249 (MQ=255)                                                                                                                                                                  gAGCCATTTTTGTCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGTATTTAATTTTGTCCTGGATAGAAGACAGAGCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGcc                                                                 <  1:197138/251‑1 (MQ=255)                                                                                                                                                                               ccAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATNAGANTCAGNGTNAGTACCGGTAGTTGCNTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCNNANNNCNCGCCTTCg                                                                                                                         <  2:149200/182‑1 (MQ=255)                                                                                                                                                                               ccAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCCGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCg                                                                                                                         >  1:149200/1‑182 (MQ=255)                                                                                                                                                                                  gCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGtn                                                 <  1:130426/251‑2 (MQ=255)                                                                                                                                                                                  ccACGTCCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCATTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTTGTCTGCGCAACGGAGATTCCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTg                                                 <  1:83589/250‑1 (MQ=255)                                                                                                                                                                                     cGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTTGTCCTGGATAGACGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTaga                                                     >  1:115916/1‑244 (MQ=255)                                                                                                                                                                                                                                  cATCCAGACGGGATTTAATTTTGTCCTGGATAGAAGACAGATCAGACTCAGAGTTCGTACCGGTCGTGGCCTGTCCCGCCAGTTCACGCACACGCTGTAACTTGTTGTTGATTTCGGACAGCGAGCACTCGGTGGTCTGTGCACCGGTGATACCGTCGTTGGCGTTCCGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGACACGGTTAGCAATCGCCTGCCCCGCTGCGTCATCCTTCGCGCTGtt  >  1:31778/1‑250 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                     TGTTTGTGGTGGTAGCACCAAAAGCAGTTACTGGAGCACTAGTGGTAACTGTATCGTTATTTTTAACGCTAAAACCATCAAGGCCAAGAGTTTTAGCATCAATCTGCTTCAGATCGATAGTGATAGTCTGGTTATCATTTGCGCCAACCTGGATTTTCATGGAGCCATTTTTTGCCAGCACGTTCACGCCGTTGAACTGGGTCTGACCAGATACGCGGTCAATTTCATCCAGACGGGATTTAATTTCGTCCTGGATAGAAGACAGATCAGACTCAGAGTTAGTACCGGTAGTGGCCTGTACCGTCAGTTCACGCACACGCTGTAAGTTGTTGTTGATTTCGGACAGCGCGCCTTCGGTGGTCTGCGCAACGGAGATACCGTCGTTGGCGTTACGGGCCGCCTGAGTCAGGCCTTTAATGTTAGAGGTGAAACGGTTAGCAATCGCCTGACCCGCTGCGTCATCCTTCGCGCTGTT  >  NC_000913/2003018‑2003492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑