Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,978,243	G→T	intergenic (‑46/+275)	flhD ← / ← insB5	DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,978,243	0	G	T	100.0%	24.0 / NA	11	intergenic (‑46/+275)	flhD/insB5	DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (7/4);  total (7/4)

CCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTC  >  NC_000913/1978008‑1978484                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  ccAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTAGTTTTATGCGGTTTCACCTCACCCCGTGATGTCg                                                                                                                                                                                                                                    <  1:59273/251‑1 (MQ=255)    gTTGATTGGGTTCTGCCAGCTTAACCCTTTGCGGAAGAGTCACTGCCGCTAACGTTGTCACAATATCGTCATTCATGCCGAGACGAAACATAGTGGATGCTTTGTCCTGCACGATCAAACGCTGTGCAGCTAGTAAACATGACACGTTGATGTCATACATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGATTCACCTCACCCCGTGATGTCGCCg                                                                                                                                                                                                                                 >  1:95375/1‑251 (MQ=255)         ttGGTTTCTGCCAGCTTAACCATTTGGGGAAGAGTCAGTGCCGCCAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCAGAACAATCAAACGCTGTGCAAGTAGGAAATATGACAAGTTGATGTTATAAATGTGTTTCAGCAACTCGGAGGGATGCATTATTACCACCCAGAATAACAAACTTTATTTTTATGCGGTTTCAACTCACCCCGTGATGttgagtacgg                                                                                                                                                                                                                             >  1:5284/1‑241 (MQ=255)                                                    cTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGCAACATAGCGGACGCGTTGTCCTGAACAATAAAACGCTGTGCAAGTCGTAAATATGACAAGTTGATGTCATCAATGTGTTTCAGCAACTCGGAGGTATGCATTCTTCCCACTCAGAATAACCAACTTTATTTTTATGCGGTTTAACCTCACCCCGTGATGTCGCCgggcag                                                                                                                                                                                                                            >  1:219942/1‑205 (MQ=255)                                                                           tttATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTAGGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCgn                                                                                                                                                                                                                      <  1:43836/191‑2 (MQ=255)                                                                                                 cGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGAt                                                                                                                                                                                                                                        <  1:12410/151‑1 (MQ=255)                                                                                                           ntCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGCCAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGCATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGCTGTCGCCGGGAAGCCCCGGTAAACAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATACGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGt                                                                                                                          >  1:42188/2‑251 (MQ=255)                                                                                                                        aaaCGCTGTGCAAGTAGTAAATATGAAAAGTTGATGTCATAAATGTGTTTCAGCCAATCGGCGGAAGGCATTATTCGCACCCAGCATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGCGATGTCGCCGGGAAGACCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATCAGCAGAACCACCTTTTTGGTTTACTATGTCCTTAc                                                                                                                                        >  1:204977/1‑224 (MQ=255)                                                                                                                                                             cATAANNGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCTCACCCCGTGATGTCg                                                                                                                                                                                                                                    <  2:5284/95‑1 (MQ=255)                                                                                                                                                                          cAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTCTGCGGTTTCACCTCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCt                                                                                                                                                                                 >  1:123334/1‑133 (MQ=255)                                                                                                                                                                                                                                    gTTTCACCTCACCCCGGGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGAAGAACCACCTTTTTGGTTTCATATGTCCTTCCAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTc  >  1:186181/1‑250 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                  CCAGTTGATTGGTTTCTGCCAGCTTAACCATTTGCGGAAGAGTCAGTGCCGCTAACGTTGTCGCCATTTCTTCATTTATGCCGAGACGAAACATAGCGGACGCTTTGTCCTGAACAATCAAACGCTGTGCAAGTAGTAAATATGACAAGTTGATGTCATAAATGTGTTTCAGCAACTCGGAGGTATGCATTATTCCCACCCAGAATAACCAACTTTATTTTTATGCGGTTTCACCGCACCCCGTGATGTCGCCGGGAAGCCCCGGTAAAAAATAATTAGCATTAGAATAGTTGCGATAAGCTGCAATAAGCAGAACCACCTTTTTGGTTTAATATGTCCTTACAAATAGAAATGGGTCTTTACACTTATCTAAGATTTTTCCTAAATCGACGCAACTGTACTCGTCACTACACGCACATACAACGGAGGGGGGCTGCGATTTTCAATAATGCGTGATGCAGATCACACAAAACACTC  >  NC_000913/1978008‑1978484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑