Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,648,423	G→A	intergenic (+82/‑85)	dicA → / → ydfA	DNA‑binding transcriptional dual regulator DicA/DUF1391 domain‑containing protein YdfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,648,423	0	G	A	100.0%	28.3 / NA	11	intergenic (+82/‑85)	dicA/ydfA	DNA‑binding transcriptional dual regulator DicA/DUF1391 domain‑containing protein YdfA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

AGTTGCCTTATCCCCCAAAGAACTAGAGCTCCTTGAGCTGTTTAATGCACTGCCAGAATCAGAACAGGATACCCAGCTCGCCGAAATGCGAGCTCGAGTAAAAAACTTCAATAAACTCTTTGAAGAATTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCGATATGTTAGTCATGGCTAATCTTGTTTGCATCAACACACCGCACGGTGTTCTCAGCAAACAGTTCCGCTACCCCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCAC  >  NC_000913/1648179‑1648575                                                                                                                                                                                                                                                     |                                                                                                                                                         aGTTGCCTTATCCCCCAAAGAACTAGAGCTACTTGAGCTGTTTAATGCACTGCCAGAATCAGAACAGGCTACCCAGCTCGCCGAAATGCGAGCTCGAGTAAAAAACTTCAATAAACTCTTTGAAGAATTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCGATATGTTAGTCATGACTAATc                                                                                                                                                    >  1:183134/1‑251 (MQ=255)                                     gCTGTTTAATGCACTGCCAGAATCAGAACAGGATACCCCGCTCGCCGAAATGCGAGCTCGAGTAAAAAACTTCAATAAACTCTTTGAAGAATTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCGATATGTTAGTCATGACTAATCTTGTTTGCATCAACCCACCGCACGGTGTTCTCAGc                                                                                                                 >  1:207267/1‑250 (MQ=255)                                                                   ggATACCCAGCTCGCCGAAATGCGAGCTCGAGTAAAAAACTTCAATAAACTCTTTGAAGACTTCCTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCGATATGTTAGTCATGACTAATCTTGTTTGCATCAACACACCGCACGGTGTTCTCAGCAAACAGTTCCGCTACCCCAGCGTTAAgggg                                                                                   >  1:31460/1‑250 (MQ=255)                                                                                         cGAGCTCGAGTAAAAAACTTCAATAAACTCTTTGAAGAATTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCGATATGTTAGTCATGACTAATCTTGTTTGCATCAACACACCGCACGGTGTTCTCAGCAAACAGTTCCGCTACCCCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACt                                                             >  1:31251/1‑250 (MQ=255)                                                                                               cGAGTAAAAAACTTCAATAAACTCTTTGAAGAATTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTAGAGCATACAAAATCACTTGACCGAGATGTTAGGCATGACTAAGCTTGTTTGCATCGACACACCGCACGGTGTTCTCAGCAAACAGTTCCGCTACCTCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGa                                                        <  1:215292/249‑1 (MQ=255)                                                                                                    aaaaaaCTTCAATAAACTCTTTGAAGAATTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCGATATGTTAGTCATGACTAATCTTGTTTGCATCAACACACCGCACGGTGTTCTCAGCAAACAGTTCCGCTACCCCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCttcg                                                  <  1:210759/250‑3 (MQ=255)                                                                                                                          gaagaaTTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCTATAGGTTAGTCATGACTAATCTTGTTTGCATCAACACACCGCACGGTGTTCTCAGCAAACAGTTCCGCTACGCCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTAc                            <  1:98208/250‑1 (MQ=255)                                                                                                                              aaTTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCGATATGTTATTCATGACTAATCTTGTTTGCATCAACACACCGCACGGTGTGCTCAGCAAACAGTTCCGCTACCCCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATc                                                          <  1:90127/216‑1 (MQ=255)                                                                                                                                                   ggACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATTCAAAATCACTTGACCGATATGTTAGTCATGACTAATCTTGTTTGCATCAACACACCGCACGGTGTTCTCAGCAAACAGTTCCGCTACCCCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCan  <  1:23526/251‑2 (MQ=255)                                                                                                                                                         nnnnnnnnnnaNATNNTCAANGAGTTATCTTTTACCACATCAATTNTGTTAGCTATAGCATACAAAATCACTTGACCNANATGTTAGTCATGACTAATCTTGTTTGCATCAACACACCGCACGGTGTTNTCAGCAAACANTTCNNCTACNCNANCGTTAAGGGGAAATGAGGTCAGCATGGATNCTATCGATCTTGGCAACAACGAANNTCNNNTGNNCGGCg                        <  2:159676/213‑1 (MQ=255)                                                                                                                                                            nnnnnnnaNATNNTCAANGAGTTAGCTTTTACCACATCAATTNTGTTAGCTATAGCATACAAAATCACTTGACCNANATGTTAGTCATGACTAATCTTGTTTGCATCAACACACCGCACGGTGTTNTCAGCAAACANTTCNNCTACNCNANCGTTAAGGGGAAATGAGGTCAGCATGGATNCTATCGATCTTGGCAACAACGAANNTCNNNTGNNCGGCg                        <  2:159687/213‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                         AGTTGCCTTATCCCCCAAAGAACTAGAGCTCCTTGAGCTGTTTAATGCACTGCCAGAATCAGAACAGGATACCCAGCTCGCCGAAATGCGAGCTCGAGTAAAAAACTTCAATAAACTCTTTGAAGAATTACTAAAAGCCCGTCAGCGGACAAATAAAAGATAACATCATCAATGAGTTATCTTTTACCACATCAATTATGTTAGCTATAGCATACAAAATCACTTGACCGATATGTTAGTCATGGCTAATCTTGTTTGCATCAACACACCGCACGGTGTTCTCAGCAAACAGTTCCGCTACCCCAGCGTTAAGGGGAAATGAGGTCAGCATGGATACTATCGATCTTGGCAACAACGAATCTCTGGTGTACGGCGTGTTTCCAAACCAGGACGGCAC  >  NC_000913/1648179‑1648575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑