Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	422,420	C→T	intergenic (+61/‑98)	proY → / → malZ	putative transporter ProY/maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,420	0	C	T	100.0%	14.3 / NA	7	intergenic (+61/‑98)	proY/malZ	putative transporter ProY/maltodextrin glucosidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/2);  total (5/2)

CTGCTCTTTATTATCGGGTTGATTGGTTATCACCCGGATACGCGTATCTCGCTGTATGTCGGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAT  >  NC_000913/422219‑422654                                                                                                                                                                                                          |                                                                                                                                                                                                                                           cTGCTCTTTATTATCGGGTTGATTGGTTATCACCCGGATACGCGTATCTCGCTGTATGTCGGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATTCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTg                                                                                                                                                                                           >  1:10399/1‑251 (MQ=255)                          gTTATCACCCGGATACGCGTATCTCGCTGTATGTCGGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGATCCGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATTCGCCACCGTCATCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTCGATTAGGCTATGGCAAGGTGATc                                                                                                                                                                    >  1:100879/1‑249 (MQ=255)                                                              gTTTCGCGNGNNNTNNTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCNGCTCGCTGAAAACCANTANTCCTNGCGNTTTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATTCGCCCCCGTCCTCCTCCCCCAAATCTCCTTCAGATGATGAGTGATCCTGCATTNGGCNATGGCNNGNGGATCANNNTTNNNNNNNNgnnnntcnnannnc                                                                                                                                        >  2:148268/1‑228 (MQ=255)                                                                                                                                     naCCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAACGTTATTCGCCCCCGTGCTCCTCCCCCAAATATACTTCAGATGATGAGTGATCCTGCATTAGCCTATGGCAAGGTGATCAGATTTTCATAACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGc                                                       >  1:18979/2‑251 (MQ=255)                                                                                                                                                                            aaaTCCTGATCCGGCTATTCCGAAAGTTATTCGCCCCCGTCCGCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAGCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAacac                <  1:141005/251‑1 (MQ=255)                                                                                                                                                                                          cTATTCCGAAAGTTATTCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGCCGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGCGAATTATGATGTTAAATGCATGGCACCTGCCGGAGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAt  >  1:188149/1‑251 (MQ=255)                                                                                                                                                                                                cGAAAGTTATTCGCCCCCGGCCTCCGCCCCCAAATATCCTTCAGATTATGAGTGATCCTGCATTAGGCTAGGGCAAGGTGATCAGATTTTCATCACAGGGGATGTATGAGTTTAGATTTATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGAGCAACt                                                                                <  1:117514/167‑1 (MQ=255)                                                                                                                                                                                                          |                                                                                                                                                                                                                                           CTGCTCTTTATTATCGGGTTGATTGGTTATCACCCGGATACGCGTATCTCGCTGTATGTCGGTTTCGCGTGGATTGTTGTGCTGTTGATTGGCTGGATGTTTAAACGCCGCCACGATCGTCAGCTGGCTGAAAACCAGTAATCCTTGCGTTCTGTAAGCCGGTTCAGGAGGAAATCCTGATCCGGCTATTCCGAAAGTTATCCGCCCCCGTCCTCCTCCCCCAAATATCCTTCAGATGATGAGTGATCCTGCATTAGGCTATGGCAAGGTGATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAAT  >  NC_000913/422219‑422654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑