Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,412,787	C→T	R150K (AGA→AAA)	intR ←	putative integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,412,787	0	C	T	100.0%	26.2 / NA	10	R150K (AGA→AAA)	intR	putative integrase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/6);  total (4/6)

CCTGAGTAAACGGCAAATTTCCACAAGTTCTGGCTCTGTCCTTTTTCACTTTCCATTAATGCATTGAATTCTGTTTTAGATAACGGATCAGGCTTTATTCTGTTTCGCTGTAATTTTTTTACTCCTTCAAATGGTTTGGTTGATATAAATCCCGACTGATACGCAAAACGCAACAGCGAACAGAGCAGGGCGATATAGTTATCAACTGTGCGCACGGTTCTTCCTTTTTTGTTGGATCTTGGATTATCCAGGTAAAGCGTTTCTCCATGCAGCAGTTCATTCCGGTAGTTTAAGATATCGCTATAACGAATATGTGATATCGGGGTACTTTCACAAATTATTATTCTGAGTGTTTTTAATTGTGATTTCGTTTTCTTCATTGTGTTTGTTGTTAACTCTGTCTCTTTAATTTTTGTCCAGATATCACAAAGCTCTCCGAACGTTTTTATGACTCTCGTTGTCACCATT  >  NC_000913/1412550‑1413017                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       acTGAGTAAACGGCAAATTTCCACAAGTTCTGGCTCTGTCATTTTTCACTTTCCATTAATGAATTGAATTCTGTTTTAGATAACGGATCAGGCTTCATTCTGCTTCGATGTAATTTTTTTACTCCTTCAGATGGTTTGGTTGATATACATTCTGACTGATACGCACAACGCAACAGCGAACAGAGCAGGGCGATATAGTTATCAACTGTGCGCACGGTTCTTCCTTTTTTGTTGGTTTTTGGATTATcc                                                                                                                                                                                                                             >  1:218679/2‑249 (MQ=255)             gCAAATTTCCACAAGGTCTGGCTCTGTCCTTTTTCTCTTTCCATTAATGCATTGAATTCTGTTTTAGATAACGGATCAGGCTTTATTCTGTTTCGCTGTAATTTTTTTACTCCTTCAAATGGTTTGGTTGATATAAATCCCGACTGAGACGCAAAACTCAACAGCGAACAGAGCAGGGCGATATAGATATCAACTGTGCGCACGGTTCTGCCTTTTTTGTTGGATTTTGGATTATCCAGGTAAAGCGTTtc                                                                                                                                                                                                               <  1:179874/251‑1 (MQ=255)                                                                                                                                              gATATAAATCCCGCCTGATACGCAAAACGCAACAGCGAACCGAGCAGGGCGATATAGTTATCAAATGTGCGCAAGGTTCTTCCTTTTTTGTTGGATTTTGGCTTATCCAGGTACAGCGTTTCTCCATGCAGCAGTTCATTCCGGTAGTCTAAGATATCGCTATAACGACTATGTGATATCGGGGTACTTTCACAAATTATTATTCTGAGTGtttt                                                                                                                  >  1:216248/1‑215 (MQ=255)                                                                                                                                                     aTCCCGACTGCTACGAAACACGCAACAGCGAACAGAACAGGGCGATATCGTTATCAACTGTGCGCACGGTTATTCCTTTTTTGTTGGATTTTGGATTATCCAGGTAAAGCGTTTCTCCATGCAGCAGTTCATTCCGGTAGTTTAAGATATCGCTATAACGAATATGTGATATCGGGGTACTTTCACAAATTATTATTCTGAGTGTTTTTAATTGTGATTTCGTTTTCTTCATTGTGTTTGTTGTTAActct                                                                       >  1:182607/1‑251 (MQ=255)                                                                                                                                                                        aCGCAACAGCGAACAGAGCAGGGCGATATAGTTATCAACTGTGCGCACGGTTCTTCCTTTTTTGTTGGATTTTGGATTATCCAGGTAAAGCGTTTCTCCATGCAGCAGTTCATTCCGGTAGTTTAAGATATCGCTATAACGAATATGTGATATCGGGGTACTTTCACAAATTATTATTCTGAGTGTTTTTAATTGTGATTTCGTTTTCTTCATTGTGTTTGTTGTTAACTCTGTCTCTTTAATTTTTGTcn                                                    <  1:129148/251‑2 (MQ=255)                                                                                                                                                                                gCGAACAGAGCAGGGCGATATAGTTATCAACTGTGCGCACGGTTCTTCCTTTTTTGTTGGATTTTGGATTATCCAGGTAAAGCGTTTCTCCa                                                                                                                                                                                                           >  1:37849/1‑92 (MQ=255)                                                                                                                                                                                            gggCGATATAGTTATCAACTGTGCGCACGGTTCTTCCTTTTTTGTTGGATTTTGGATTATCCAGGTAAAGCGTTTCTCCATGCAGCAGTTCATTCCGGTAGTTTAAGATATCGCTATAACGAATATGTGATATCGGGGTACTTTCACAAATTATTATTCTGAGTGTTTTTAATTGTGATTTCGTTTTCTTCATTGTGTTTGTTGTTAACTCTGTCTCTTTAATTTTTGTCCAGATATCACAAAGCTCTCCg                                <  1:86325/251‑1 (MQ=255)                                                                                                                                                                                                         aTCAACTGTGCGCACGGTTCTTCCTTTTTTGTTGGATTTTGGAGTATCCAGGTAAAGCGGTTCTCCATGCAGCAGTTCATTCCGGTAGTTTAAGATATCGCTATAACGAAGATGTGATATCGGGGTACTTTCACAAATTATTATTCTGAGTGTTTTTAATTGTGATTTCGTTTTCTTCATTGTGTTGGTTTGTAACGCTGTCTCTTTAATTGTTGTCCAGATATCACAAAGCTCTCCGAACGTTTTTAtt                    <  1:163703/250‑2 (MQ=255)                                                                                                                                                                                                             ncngggcgcACGGTTCTTCCTTTTTTGTTGGATTTTGGNTGANNCANNGANAGCGt                                                                                                                                                                                                                  <  2:124670/51‑1 (MQ=255)                                                                                                                                                                                                                           tcttcCTTTTTTGTGGGATTTTGGATTATCAAGGTAAAGCGTTTCTCCATGCAGCAGTTCATTCCGGTAGTTTAAGATATCGCTATAACGAATATGTGATATCGGGGTACTTTCACAAATTATTATTCTGAGTGTTTTTAATTGTGATTGCGTTTTCTTCATTGTGTTTGTTGTTATCTCTGTCTCTTTAATTTTTGTCCAGATATCACAAAGCAGTCCTAATGTTTGTAGGACTCTCGTGGTCGCCAtt  <  1:172862/250‑1 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                       CCTGAGTAAACGGCAAATTTCCACAAGTTCTGGCTCTGTCCTTTTTCACTTTCCATTAATGCATTGAATTCTGTTTTAGATAACGGATCAGGCTTTATTCTGTTTCGCTGTAATTTTTTTACTCCTTCAAATGGTTTGGTTGATATAAATCCCGACTGATACGCAAAACGCAACAGCGAACAGAGCAGGGCGATATAGTTATCAACTGTGCGCACGGTTCTTCCTTTTTTGTTGGATCTTGGATTATCCAGGTAAAGCGTTTCTCCATGCAGCAGTTCATTCCGGTAGTTTAAGATATCGCTATAACGAATATGTGATATCGGGGTACTTTCACAAATTATTATTCTGAGTGTTTTTAATTGTGATTTCGTTTTCTTCATTGTGTTTGTTGTTAACTCTGTCTCTTTAATTTTTGTCCAGATATCACAAAGCTCTCCGAACGTTTTTATGACTCTCGTTGTCACCATT  >  NC_000913/1412550‑1413017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑