Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,410,575	C→T	I355I (ATC→ATT)	dbpA →	ATP‑dependent RNA helicase DbpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,410,575	0	C	T	100.0%	14.9 / NA	7	I355I (ATC→ATT)	dbpA	ATP‑dependent RNA helicase DbpA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/3);  total (4/3)

GGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCACAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGAAAAAAGCCAAAATGCGCCCGGGTGATGTATTAGGTGCACTGACAGGAGATATCGGGCTTGATGGCGCAGATATTGGCAAAATCGCCGTGCA  >  NC_000913/1410362‑1410772                                                                                                                                                                                                                      |                                                                                                                                                                                                      ggTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGACTGATGTTGCTGAGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCACAGCGGGCCAATATTATTTCTGACATGTTGCAGATAAAACTTAACTGGCaa                                                                                                                                                                   >  1:193779/1‑250 (MQ=255)                             gtACTGGTCGCGACTGATGTTGTTGTGGGTGGTCTGGATATTAATGCGCTTGAGCTTGTGGGGAACTTTGAGCGGGCGTGGGACCCTGAAGTTCATGGACGTCGCATCGGTCGTACAGCTCGTGCAGGTAATAGCGGTCGGGCGATCAGTTTCTGGGCTCCGGAAGAAGCACAGCGCTCCAATATTATATCCGACATGTGGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGa                                                                                                                                       <  1:210662/250‑1 (MQ=255)                                               gTTGCTGCGCGTGGTCTGGATATTAAATCGCGTGAGCGGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACCTCGCATCGGTCGTACATCTCGTGCAGGAAATATCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCACAGCGGGCCAATATTATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATggn                                                                                                                    <  1:137009/251‑2 (MQ=255)                                                        cgTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGGCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCACAGCGGGCCAATATTATTGCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTGCCATTGCGACGCTGGAAGCAGAAATGGCAACGTtgtg                                                                                                           <  1:199834/251‑1 (MQ=255)                                                          tGGTCTGGATATTAAATCGCTTGAGCTGGAGGTGAACTTTGCGCTGGCGTGAGACCCTGCAATTACTGTACATAGCATCGGTCGAACCGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCAGGACGAAGCACAGCGGGCCAATATTATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTCATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTgtgt                                                                                                          >  1:222038/1‑250 (MQ=255)                                                                                                                                                     cGTGCAGGAAATCGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCACAGCGGGCCAATATTATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCCTTGCGACGATGGAAGCAGAAATGGCAACGTTGTGTATCGATGGTGGGAAAAAAGCCAAAATGCGCCCGGGTGATGTATTAGGTGCACTGACAGga                                              >  1:115933/1‑219 (MQ=255)                                                                                                                                                                                                              gCCAATATTATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGCCGCTGGCAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGAAAAAAGCCAAAATGCGCCCGGGTGATGTATTAGGTGCACTGACAGGAGATATCGGGCTTGATGGCGCAGATATTGGCAAAATCGCCGTGCa  >  1:36827/1‑206 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                      GGTACGTTTTGCTAACGGTAGCGCCCGTGTACTGGTCGCGACTGATGTTGCTGCGCGTGGTCTGGATATTAAATCGCTTGAGCTGGTGGTGAACTTTGAGCTGGCGTGGGACCCTGAAGTTCATGTACATCGCATCGGTCGTACAGCTCGTGCAGGAAATAGCGGTCTGGCGATCAGTTTCTGTGCTCCGGAAGAAGCACAGCGGGCCAATATCATTTCTGACATGTTGCAGATAAAACTTAACTGGCAAACGCCGCCAGCTAATAGTTCCATTGCGACGCTGGAAGCAGAAATGGCAACGTTGTGTATCGATGGCGGGAAAAAAGCCAAAATGCGCCCGGGTGATGTATTAGGTGCACTGACAGGAGATATCGGGCTTGATGGCGCAGATATTGGCAAAATCGCCGTGCA  >  NC_000913/1410362‑1410772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑