Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,394,793	C→T	P523S (CCT→TCT)	mppA →	murein tripeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,394,793	0	C	T	100.0%	31.0 / NA	13	P523S (CCT→TCT)	mppA	murein tripeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/8);  total (5/8)

CTCCACTTTCCTGACATTATTAACGTCAACGCATTCAGGAAATATTTCACGCTTTAACAATCCGGCATATGACAAAGTTCTGGCCCAGGCATCGACGGAAAATACCGTTAAAGCGCGTAATGCCGATTACAACGCGGCAGAAAAAATCCTCATGGAGCAAGCACCGATTGCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAG  >  NC_000913/1394555‑1395016                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                cTCCACTTTCCTGACATTATTAACGTCAACGCATTCAGGAAATATTTCAAGCTTTAACAATCCGGCATATGACAAAGTTCTGGCCCAGGCATCGACGGAAAATACCGTTCAAGCGCGTAATGCCGATTACAACGCGGCAGAAAAAATCCTCATGGAGCAAGCACCGATTGCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATTCTGAAGATGTgg                                                                                                                                                                                                                     >  1:45257/1‑251 (MQ=255)                                                                                               aCGGAAAATACCGTTAAAGCGCGTAATGCCGATTACAACGCGGCAGAAAAAATCCTCATGGAGCAAGAACCGATTGAACCAATTTATCAATATACCAATGGACGATTCATCAAGCCGTGGCTGAAAGGTTATCCCATTACTAATTCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGAcc                                                                                                                       >  1:30543/1‑251 (MQ=255)                                                                                                                  ngcgTAATGCCGATTACAACGCGGCAGAAAAAATCCTCATGGAGCAAGCACCGATTGCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATTCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATg                                                                                                    >  1:61422/2‑251 (MQ=255)                                                                                                                                            gAAAAAATCCTCATGGAGCAAGCACCGATTGCACCAATTTATCAATATAACAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATTCTGACGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGc                                                                                                                                                     >  1:116771/1‑176 (MQ=255)                                                                                                                                                        aTGGAGCAAGCACCGATTGCACCTATTTATCAAGATTCCAATGGACGATTAAACAAGCCGTGGCTGAAAGGTTATCCCATTAATAATTCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTGCCCTTGTGCAAAACGCCATTGAGGTCTGAGTTAGCGACCCTGTGGCTGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTc                                                              <  1:187446/251‑1 (MQ=255)                                                                                                                                                                agcaCCGATTGCACCAATTTATCAATATCCCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATTCTGCAGATGTGGCGTACAGTCGGACTATGTATATTGTGACGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCgt                                                      >  1:67978/1‑251 (MQ=255)                                                                                                                                                                                                 tGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATGAATAATTCTGAAGAGGTGGCGTACAGTCGGACTATGGATATTGTGAAGCATTGATGTGATGGGAACTTGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAg                                                                                                                            <  1:176255/148‑1 (MQ=255)                                                                                                                                                                                                         tAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATTCGGAAGATGTGGCTTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTAc                                                                                                                                                             <  1:88920/107‑1 (MQ=255)                                                                                                                                                                                                          aaTCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATTCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGT‑AAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCtg           <  1:177067/251‑1 (MQ=255)                                                                                                                                                                                                                  cGTGGCTGAAAGGTTATCCCATTAATAATTCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGc                                                                                                                           <  1:15838/132‑1 (MQ=255)                                                                                                                                                                                                                     ggCTGAAAGGTTATCCCATTAATAATTCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAg  <  1:10434/250‑1 (MQ=255)                                                                                                                                                                                                                       ntGNAAGNTNNTNNCATNAATAATTCTGAAGATGTGGCGNNCAGTCGGACNATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATANCGCCAGTGANNTCNGATTTNGNNNCNNNGTNCCGGCGGTGTAATGTTATCNATNNACAGCGNCTGGCTGGGGAAGGCAAAGTCCgcgc                                                         <  2:45257/192‑1 (MQ=255)                                                                                                                                                                                                                                     naTTAATNNTTCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAg                                                                                                                                           <  2:3410/96‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                CTCCACTTTCCTGACATTATTAACGTCAACGCATTCAGGAAATATTTCACGCTTTAACAATCCGGCATATGACAAAGTTCTGGCCCAGGCATCGACGGAAAATACCGTTAAAGCGCGTAATGCCGATTACAACGCGGCAGAAAAAATCCTCATGGAGCAAGCACCGATTGCACCAATTTATCAATATACCAATGGACGATTAATCAAGCCGTGGCTGAAAGGTTATCCCATTAATAATCCTGAAGATGTGGCGTACAGTCGGACTATGTATATTGTGAAGCATTGATGTGATGGGAACTGGCGTTACCCTTGTGCATAACGCCAGTGATGTCTGATTTAGCGACCCTGTTCCGGCGGTGTAATGTTATCCATATACAGCGTCTGGCTGGGGAAGGCAAAGTCCGCGCCGTGTGACTGTACAATATCGATAATCTTCAAATAAACGTCTTGCTGTGCAGCAAG  >  NC_000913/1394555‑1395016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑