Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,353,758	T→A	D254V (GAT→GTT)	sapC ←	putrescine ABC exporter membrane protein SapC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,353,758	0	T	A	100.0%	23.3 / NA	11	D254V (GAT→GTT)	sapC	putrescine ABC exporter membrane protein SapC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/5);  total (6/5)

ACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATG  >  NC_000913/1353552‑1353926                                                                                                                                                                                                               |                                                                                                                                                                         ncccGGTCGACGGCTTTAACCCAATAATAAACGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTACACAACCGCAATACTCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTTCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTtcgc                                                                                                                                >  1:127502/2‑249 (MQ=255)     ggTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACACCACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCg                                                                                                                              >  1:212449/1‑247 (MQ=255)     ggTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCg                                                                                                                              >  1:184825/1‑247 (MQ=255)                                                     ggTTACGAATATCGCGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGCGGGGAGTTGTGCGCTGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTgcgc                                                                          >  1:72009/1‑251 (MQ=255)                                                                                    ccccccTCAATAAGCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCTCCTGTCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGATGGGAGTTGTGCGCCTAGATCGACATAGCCCTTCGCGTCCATATCGAGTATTGCCATCGTCAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGAt                                           <  1:187060/250‑1 (MQ=255)                                                                                    ccccccGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAAGCAGTTCCAGCGCAACACCGATCATCGCTCCCCATTCACGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCCCGGGTGATCGCGGTGACGAGCCCGGCGGTGAt                                           <  1:183500/250‑1 (MQ=255)                                                                                       cccGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGtt                                        >  1:141544/1‑251 (MQ=255)                                                                                                                      cAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCGCCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGc                                                                                                                                                                  <  1:109324/98‑1 (MQ=255)                                                                                                                             gTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCTCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGGTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGAtn  <  1:19619/251‑2 (MQ=255)                                                                                                                                                         nccNCACCTNGCAGNATGACAGTCCACGGGNCGACATAAATCAGTTCCATNNCNACACCNAGCAt                                                                                                                                                                <  2:79275/64‑1 (MQ=255)                                                                                                                                                         gCCGCACCTGGCCGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCAACACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACCGCAAAc                        >  1:15355/1‑201 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                         ACCCGGTCGACGGCTTTAACCCACTCATCACCGGTTTTAAATTCAATGGTCAGGTTACGAATATCGAGTAATGGCATTATTCCACCCCCGCAATAATCGCACGACGAACGCCATCGCCAAGGAGGTTAACCAACAACACGCTAATCATAATTGCCGCACCTGGCAGCATGACAGTCCACGGGGCGACATAAATCAGTTCCAGCGCATCACCGAGCATCGCTCCCCATTCAGGCGAGGGGAGTTGTGCGCCGAGATCGAGAAAGCCCAGCGCGGCGATATCGAGAATTGCCATCGACAGTGCGCGGGTGATCTCGGTGACCAGCCCGGCGGTGATGTTTGGCATCACAGCAAACCAGAGAATATTCAGCGTTGATG  >  NC_000913/1353552‑1353926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑