Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,337,394	A→G	S522G (AGC→GGC)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,337,394	0	A	G	100.0%	19.9 / NA	11	S522G (AGC→GGC)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/6);  total (5/6)

GCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC‑CGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAG  >  NC_000913/1337159‑1337631                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               gCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAATGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGtn                                                                                                                                                                                                                                  <  1:128660/250‑2 (MQ=255)                                      aCTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTCTCTGGCAAAAGCGAACCTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGCTCCTATCGCAACGGCAATCAAAAACGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC‑CGTAACTTTGAAGGCCg                                                                                                                                                                                           >  1:110101/1‑251 (MQ=255)                                                                                   ccGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAAATGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGATGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC‑CGTAACTTTGAAGGCCGGATCCATCCGCTGGTTAAAACAAACTGGATGGCATCGACGCCGCt                                                                                                                                              >  1:167669/1‑251 (MQ=255)                                                                                                  gTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC‑CGTAACTTTGAAGGCCGTATCCATCCGCTTGTTAAAACTAACTGGCTGGCCTCGCCGCCGCtggtgct                                                                                                                                        <  1:206069/242‑3 (MQ=255)                                                                                                                  cAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC‑CGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAActgn                                                                                                                                                               <  1:55964/203‑2 (MQ=255)                                                                                                                                            gacgaAATGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGACCGATCATATCGAAACGGCAATCAAAAAAGGCGAtt                                                                                                                                                                                                                                          >  1:164457/1‑103 (MQ=255)                                                                                                                                                  cTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAc                                                                                                                                                                                                                                      >  1:12240/1‑101 (MQ=255)                                                                                                                                                    gnngTTTAANNTTGTGGGATACGCTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGCTCCTATCGAAACGGCAATCAAAAAAGGCGATTTAAc                                                                                                                                                                                                                                      <  2:12240/99‑1 (MQ=255)                                                                                                                                                                           gTTGTACCACCTGTATGGGGAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGAGTTAACCGGCGGTGCGGTGCTGTCGGGCAACGCG‑ACCGTTGAAGGCCGTATCCATCCGCTCGTTAAAACTAACTGGCTGGCCTcgcc                                                                                                                                                    <  1:186351/157‑1 (MQ=255)                                                                                                                                                                                             gTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAGGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC‑CATCACTTTGAAGGCCGCATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGACGCCGCTGGTGGATGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGACATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGcc                                     >  1:110622/1‑250 (MQ=255)                                                                                                                                                                                                                               ggCAATCAAAAAAGGCGATTTATCCGTCGGTGCGGTGCTGTCCGGCATC‑CGTAACGTTGAAGGCCGTATCCATCCGCTTGTTAAAACTAACTGGCTGGCCTCTCCTCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCTGTCAGGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAg  <  1:146730/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               GCTGATGGCCGCAGGCTTGCTGGCGAAAAAAGCCGTAACTCTGGGCCTCAAGCGGCAACCATGGGTCAAAGCGTCGCTGGCACCGGGTTCGAAAGTCGTTTCTGATTATCTGGCAAAAGCGAAACTGACACCGTATCTCGACGAACTGGGGTTTAACCTTGTGGGATACGGTTGTACCACCTGTATTGGTAACTCTGGGCCGCTGCCCGATCCTATCGAAACGGCAATCAAAAAAAGCGATTTAACCGTCGGTGCGGTGCTGTCCGGCAAC‑CGTAACTTTGAAGGCCGTATCCATCCGCTGGTTAAAACTAACTGGCTGGCCTCGCCGCCGCTGGTGGTTGCCTATGCGCTGGCGGGAAATATGAATATCAACCTGGCTTCTGAGCCTATCGGCCATGATCGCAAAGGCGATCCGGTTTATCTGAAAGATATCTGGCCATCGGCACAAGAAATTGCCCGTGCGGTAGAACAAG  >  NC_000913/1337159‑1337631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑