Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,290,881	C→T	L214F (CTT→TTT)	rssB →	regulator of RpoS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,290,881	0	C	T	100.0%	32.6 / NA	12	L214F (CTT→TTT)	rssB	regulator of RpoS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/4);  total (8/4)

CTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGAC  >  NC_000913/1290667‑1291095                                                                                                                                                                                                                       |                                                                                                                                                                                                                       cTGGGATGCAATGGATGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTAAAACAGCAGGTACAGCAGGAGATTTCCAGTTGCAGGGTTACTTATCGTCAATTGGATGCCGCGGACACACCCGGCCTGGTGCTTGATAATGCCGCACTTTCGGAAAACGATCTGGAATTTTATTGCATTGATGACACACGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTCTTACGCGCATTGTTTAACGGat                                                                                                                                                                                    >  1:30350/1‑251 (MQ=255)                                                       aCAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCACTTGGTTGACGCGGACAACCCCGGCCTGGTGCTTGATATTGACGAACTTTAGGAAAACGATCTGGCCTTTTATTGCCTTGATGTCAACAGAGCTGGATATAATGGCGTATTTCCTGCCTAGTTATTACGAGCATTGTTTAACGGATTATTACAGAAGAAGCTTGCACACCAAAATCAAAGGTTGCAAGAGTTGGgcgagt                                                                                                                              >  1:211537/1‑248 (MQ=255)                                                                                                                 gCCGCGGACAAACCTGGCCGGGTGCTTGATATTGCCGCCCTTTCGGAAAACGATCTGGCATGTTATTTCCTTGATTTCACCCGAGCTGGACATAATGTCGTATTTGCTGCCTTTTTATTACGCGCATTGTTTAACGGATTATTATAGTATCAGCTTGCACCCCAAAAGCACCGGTGGGCAGAGTTCGGCTCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTccac                                                                     <  1:27809/250‑3 (MQ=255)                                                                                                                         naaaCCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGc                                                                     >  1:44899/2‑242 (MQ=255)                                                                                                                                        gCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCCTTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAAACAACGGTTGCCAGAGTTGGGCGCGTTATTAAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATcgcg                                             >  1:184053/1‑251 (MQ=255)                                                                                                                                            gATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGaan                                          <  1:20886/250‑2 (MQ=255)                                                                                                                                                     gCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCa                                                                                               >  1:9380/1‑188 (MQ=255)                                                                                                                                                             ggAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGCTTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTgg                        >  1:120103/1‑251 (MQ=255)                                                                                                                                                                         ggCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACCCCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTGCCGCTATTAGTTGGCTAGTAGCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCggn              <  1:38262/249‑2 (MQ=255)                                                                                                                                                                                    gCCTTGATGTCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGAc  >  1:141743/1‑250 (MQ=255)                                                                                                                                                                                            ntCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAAc                                                                                                             >  1:136170/2‑135 (MQ=255)                                                                                                                                                                                            gNCACCCGAGCTGGACATAATGGCGTATTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAAc                                                                                                             <  2:136170/135‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                       CTGGGATGCAATGGTTGATAACCCTGCCGCAGCGGCGAAATTATTACAGGAACTACAACCGCCGGTTCAGCAGGTGATTTCCCATTGCCGGGTTAATTATCGTCAATTGGTTGCCGCGGACAAACCCGGCCTGGTGCTTGATATTGCCGCACTTTCGGAAAACGATCTGGCATTTTATTGCCTTGATGTCACCCGAGCTGGACATAATGGCGTACTTGCTGCCTTGTTATTACGCGCATTGTTTAACGGATTATTACAGGAACAGCTTGCACACCAAAATCAACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTTCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAATGCGAC  >  NC_000913/1290667‑1291095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑