Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,132,514	A→G	T221A (ACC→GCC)	flgD →	flagellar biosynthesis, initiation of hook assembly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,132,514	0	A	G	100.0%	30.3 / NA	12	T221A (ACC→GCC)	flgD	flagellar biosynthesis, initiation of hook assembly
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/3);  total (9/3)

GATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCACCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGATTAAACGCTGCCGCCACCAACCTCGATGTTATTGGCAACAATATCGCCAACTCCGCCACCTACGGCTTTAAATCAGGCACGGCCTCTTTTGCCGATATGTTTGCCGGTTCGAAAGTGG  >  NC_000913/1132274‑1132718                                                                                                                                                                                                                                                 |                                                                                                                                                                                                             gATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGACCTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGCACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCt                                                                                                                                                                                                    >  1:119278/1‑251 (MQ=255)             gcgGTTGTGCGCACCATTGATATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACCACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCTCGACGAAGTACg                                                                                                                                                                                        >  1:137831/1‑251 (MQ=255)                                             gACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAg                                                                                                                                                            <  1:624/247‑1 (MQ=255)                                                                                cGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGAtt                                                                                                                      >  1:69230/1‑250 (MQ=255)                                                                                        gATGGCAGCGCTGCGCCGAACGGTTCTTAAAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAAGTGTGGGCCCAGCCGCGGCAGTTTGCTCTGGTGCAGGGTGTGATCGGCGGCAACAGCTGTAATACGCTGTATCTCGGTATTTACTGCGTCATCGCCGTCGGCGAAGTACTGCATATAATTGAAGGCTTGACAGTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCTGTTAGCGGATTAAAc                                                                                                                  <  1:35318/246‑1 (MQ=255)                                                                                                        agAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGATTAAACGCTGCCGCCACCAACCTCGAt                                                                                             >  1:59210/2‑251 (MQ=255)                                                                                                                                   gcgcCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGCGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGATTAAACGCTGCCGCCACCAACCTCGATGTTATTGGCAACAATATCGCCAACTcc                                                                  >  1:90925/1‑251 (MQ=255)                                                                                                                                     gcCAGTACCGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGATTAAACGCTGCCGCCACCAACCTCGATGTTATTGGCAACAATATCGCCACCTCCGc                                                                >  1:32779/1‑251 (MQ=255)                                                                                                                                                          cTGGTTGCCCAGCCGCTGCAGTTTGTTATCGTCCAGAGTCTGATCCGCGGCAACAGCGGCACTACGCTGGATATCCGCACTTACGGCGCCACCACCCTCGACGACGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGATTAAACGCTGCCGCCACCAACCTCGATGTTATTgg                                                                                     >  1:68701/1‑209 (MQ=255)                                                                                                                                                                 nccAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGATTAAACGCTGCCGCCACCAACCTCGATGTTATTGGCAACAATATCGCCAACTCCGCCACCTACGGCTTTAAATCAGGCACGGcc                                    >  1:63434/2‑251 (MQ=255)                                                                                                                                                                 cccAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGAGGTAATACGCTGGATCTCGGCACTTACGGCGCCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGATTAAACGCTGCCGCCACCAACCTCGATGTTATTGGCAACAATATCGCCAACTCCGCCACCTACGGCTTTAAATCAGGCACGGcc                                    >  1:153627/1‑251 (MQ=255)                                                                                                                                                                                                    aTCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACGTACGGCGCCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACAGTTCAGGAGTCAGTCATGGCCTTGTCTCAAGCGGTTAGCTGATTAAACGCTGCCGCCACCAACCTCGATGTTATTGGCAACAATATCGCCAACTCCGCCACCTTCGGCTTTAAATCAGGCACGGCCTCTTTTGCCGATATGTGTGCCGGTTCGAAAGTgn  <  1:18736/250‑2 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                             GATAAAAATGGCGCGGTTGTGCGCACCATTGATATTGGTGAACTGACCGCCGGAGTTCACAGTTTCACCTGGGACGGTACGTTGACTGATGGCAGCACTGCGCCGAACGGTTCTTACAATGTAGCGATTAGCGCCAGTAACGGTGGTACACAACTGGTTGCCCAGCCGCTGCAGTTTGCTCTGGTGCAGGGTGTGATCCGCGGCAACAGCGGTAATACGCTGGATCTCGGCACTTACGGCACCACCACCCTCGACGAAGTACGGCAGATAATTTAAGCCTTCACATTTCAGGAGTCAGTCATGGCCTTTTCTCAAGCGGTTAGCGGATTAAACGCTGCCGCCACCAACCTCGATGTTATTGGCAACAATATCGCCAACTCCGCCACCTACGGCTTTAAATCAGGCACGGCCTCTTTTGCCGATATGTTTGCCGGTTCGAAAGTGG  >  NC_000913/1132274‑1132718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑