Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,119,030	G→A	T6I (ACC→ATC)	yceJ ←	putative cytochrome b561 YceJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,119,030	0	G	A	100.0%	19.5 / NA	9	T6I (ACC→ATC)	yceJ	putative cytochrome b561 YceJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

GAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCGTTCAGGGGTATTTGTGAATGACATAAGTGCATCCAAAACGAATTTGCGAGAGAAAGAAAATGGCGTGTCGGAGAATGAATTGCAACACAAAAATAACAAATTGAATGATATTTATTTTTATTTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAG  >  NC_000913/1118788‑1119225                                                                                                                                                                                                                                                   |                                                                                                                                                                                                    gAGTCATGTGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCGTTCAGGGATATTtgtg                                                                                                                                                                                             <  1:6342/251‑1 (MQ=255)                                         agaaagaccacGCCACAGAACACGAATAACCATCCCCATCATTAACAAAATACCGATGCTTTTAGGCAGTTCGGGTGCTTTGTGATCCCAGCCATCGTAATAACGGAGCGTGACCACCCACATACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACCCCATAGCGTTCAGTGATATTTGTGAATGACATAAGTTCATCCAAAACGAATTTGCGAGagaaag                                                                                                                                                     <  1:79321/243‑1 (MQ=255)                                                      cacaGAACGCGAATAACCAGCCCCATCATTAACAAAATAACGCTGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCGTTCAGGGATATTTGTGAATGACATAAGTGCATCCAAAACGAATTTGCGAGAGAAAGAAAATGGCGTGTc                                                                                                                                        >  1:36033/1‑251 (MQ=255)                                                                                                                         tgtgATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCGTTCAGGGATATTTGTGAATGACATAAGTGCATCCAAAACGAATTTGCGAGAGAAAGAAAATGGCGTGTCGGAGAATGAATTGCAACACAAAAATAACAAATTGAATGATATTTATTTTTATTTCAATAATTTTGa                                                                      >  1:4479/1‑250 (MQ=255)                                                                                                                                                            aTCCACAGACCTAAAGCAAACATGCCGTAGACAATCAATGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCGTTCAGGGATATTTGTGACTGACATAAGTGCATca                                                                                                                                                                           >  1:186751/1‑113 (MQ=255)                                                                                                                                                                   naCCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGCTATAACACCATAGCGTTCAGGGATATTTGTGAATGACATAAGTGCATCCAAAACGAATTTGCGAGAGAACGAAAATGGCGTGTCGGAGAATGAATTGCAACACAAAAATAACAACTTGAATGATATTTATTTTTATTTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCAt                           >  1:23075/2‑251 (MQ=255)                                                                                                                                                                                            aataatTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCGTTCAGGGATATTTGTGAATGACATAAGTGCATCCAAAACGAATTAGCGAGAGAAAGAAAATGGCGTGTCGGAGAATGAATTGCAACACAAAAATAACAAATTGAATGATATTTATTTGTATTTCAATAATTTTGAATTAATAATGAAAGAATCGTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAg  <  1:196843/251‑1 (MQ=255)                                                                                                                                                                                                 ttGCGNNTANNNAANNGAAGGCCGCAGATATAACACCATNGCGTTCAGGGATATTTGTGAATGACATAANTNCNTCCANNACGNATTTGCGAGANAAAGAAAATGGCGTGTCGGAGAATGAATTGCAACACAAAAATAACAAANTNAATGATATTTATTTTTATTTCAATAATTTTGNATTAATAATGAAAGAATCTTCATANGTTTNNTgngn                                  >  2:162638/1‑213 (MQ=255)                                                                                                                                                                                                          gCCAATGGAAGGCCGCAGATATAACACCATAGCGTTCAGGGATATTTGTGAATGACATAAGTGCATCCAAAACGAATTTGCGAGAGAAAGAAAATGGCGTGTCGGAgaatg                                                                                                                                >  1:176362/1‑111 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                    GAGTCATGGGCGAATAACTCGGCAGCGGACCCGGCGGTGGAGATATGACACGCCACAGAACGCGAATAACCAGCCCCATCATTAACAAAATACCGATGCTTTTATGCAGTTCGGGTGCTTTGTGATACCAGCCATCGTAATAACTGAGCGTGACCATCCACAGACCTAAAGCAAACATGCCGTAGACAATAATTGCGCTTAGCCAATGGAAGGCCGCAGATATAACACCATAGCGTTCAGGGGTATTTGTGAATGACATAAGTGCATCCAAAACGAATTTGCGAGAGAAAGAAAATGGCGTGTCGGAGAATGAATTGCAACACAAAAATAACAAATTGAATGATATTTATTTTTATTTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAG  >  NC_000913/1118788‑1119225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑