Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,080,523	G→A	G407S (GGC→AGC)	putP →	proline:Na(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,080,523	0	G	A	100.0%	21.7 / NA	9	G407S (GGC→AGC)	putP	proline:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/7);  total (2/7)

ATTGCCGGGATTCTGCTGTCGGCAATTCTGGCGGCGGTAATGTCAACCTTAAGTTGCCAGCTGCTGGTGTGCTCCAGTGCGATTACCGAAGATTTGTACAAAGCGTTTCTGCGTAAACATGCCAGCCAGAAAGAGCTGGTGTGGGTAGGGCGTGTGATGGTGCTGGTGGTGGCGCTGGTGGCGATTGCGCTGGCGGCAAACCCGGAAAACCGCGTGCTGGGCTTAGTGAGCTACGCGTGGGCAGGCTTTGGCGCGGCGTTTGGTCCAGTGGTGCTGTTCTCGGTGATGTGGTCACGCATGACGCGTAACGGTGCGCTGGCGGGGATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATTATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCC  >  NC_000913/1080280‑1080731                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                 aTTGCCGGGATTCTGCTGTCGGCAATTCTGGCGGCGGTAATGTCAACCTTAAGTTGCCAGCTGCTGGTGTGCTCCAGTGCGATTACCGAAGATTTGTACAAAGCGTTTCTGCGTAAACATGCCAGCCAGAAAGAGCTGGTGTGGGTAGGGCGTGTGATGGTGCTGGTTGTGGCGCTGTTGGCGATTGCGCTGGCGGCAAACTCGGAAAACCGCATGCTGGGCTTAGTGAGCTACGCGTGGGCAAGCTTTgg                                                                                                                                                                                                           <  1:144232/251‑1 (MQ=255) aTTGCCGGGATTCTGCTGTCGGCAATGCTGGCGGCGGTAATGTCAACCTTAAGTTGCCAGCTGCTGGTGTGCTCCAGTGCGATTACCGAAGATTTGTACAAAGCGTTTCTGCGTAAACATGCCAGCCAGAAAGAGCTGGTGTGGGTAGGGCGTGTGATGGTGCTGGTGGTGGCGCTGGTGGCGATTGCGCTGGCGGCAAACCCGGAAAACCGCGGGCTGGGCTTAGTGAGCTACGCGTGGGCAAGCTTTgg                                                                                                                                                                                                           <  1:71303/251‑1 (MQ=255)                                                           aGCTGCTGGTGTGCTCCAGTGCGATTACCGAAGATTTGTACAAAGCGTTTCTGCGTAAACATGCCAGCCAGAAAGAGCGGGTGTGGGTAGGGCGTGTGATGGTGCTGGTGGTGGCGCTGGTGGCGATGGCGCTGGCGGCAAACCCGGAAAACCGTGTGCTGGGCTTAGTGAGCTACGCGTGGGCAAGCTTTGGCGCGGCGTTTGGTCCAgtgg                                                                                                                                                                                       <  1:203555/213‑1 (MQ=255)                                                                               gCGATTACCGAAGATTTGTACAAAGCGTTTCTGCGTAAACATGCCAGCCAGAAAGAGCGGGTGTGGGTAGGGCGTGTGATGGTGCTGGTGGTGGCGCTGGTGGCGATTGCGCTGGCGGCAAACCCGGAAAACCGCGTGCTGGGCTTAGTGAGCTTCGCGTGGGCAAGCTTTGGCGCGGCGTTTGGTCGAGTGGTGCTGTTCTCGGTGATGTGGTCACGCATGACGCGTAACGGTGCGCTGGCGGGgatga                                                                                                                              <  1:84150/250‑1 (MQ=255)                                                                                                             cTGCGTAAACATGCCAGCCAGAAAGAGCTGGCGGGGGCAGGGCGTGTGCTGGTGCTGGTGGTGGAGATGGGGGGGATTGCGCCGGCGGCCAACGCGGAAAACCGCGTGCGGGGCTTAGTGAGCTGAGCGAGGGCAAGCTTTGGCGCGGCGTTTggac                                                                                                                                                                                             >  1:105710/1‑155 (MQ=255)                                                                                                                 gTAAACATGCCAGCCAGAAAGAGCTGGTGTGGGTATGGCGTGTGATGGTGCTGGTGGTGGCGCTGGTGGCGATTGCGCTGGCGGCAAACCCGGAAAACCGCGTGCTGGGCTTAGTGAGCTACGCGTGGGCAAGCTTGGGCGCGGCGTTTGGTCCAGTGGTGCTGTTCTCGGTGATGTGGTCACGCATGACGCGTAACGGTgcg                                                                                                                                           <  1:155191/203‑1 (MQ=255)                                                                                                                             gccagAAAGAGCTGGTGTGGGTAGGGCGTGTGATGGTGCTGGTGGTGGCGCTGGTGGCGATTGCGCTGGCGGCAAACCCGGAAAACCGCGTGCTGGGCTTAGTGAGCTACGCGTGGGCAAGCTTTGGCGCGGCGTTTGGTCCAGTGGTGCTGTTCTCGGTGATGGGGTCACGCATGACGCGTAACGGTGCGCTGGCGGGGATGATCATCGGTGCGCTGACGGt                                                                                                           <  1:149593/223‑1 (MQ=255)                                                                                                                                                            gatggtgttgtggttggCGCTGGGGGCGATTGCGCTGGCGGCAAACCCGGAAAACCGCGTGTTGGTCTTAGTGAGCTACGCGTGGGCAAGCTTTGGCGCGGCTGTTGGTCCAGTGGTGCGGTTCTCGGTGATGTGGTCACGCATGACGCGTAACGGTTCGCTTGCGGGGATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATTATTCCGGGCTTTATc                                                 <  1:154115/236‑1 (MQ=255)                                                                                                                                                                                                          cnGNAAAACCGCGTGCTGGGCTTAGTGAGCTACGCGTGGGCTNCCTTNGNNCCNGNGTNTGGTc                                                                                                                                                                                             <  2:105710/64‑1 (MQ=255)                                                                                                                                                                                                          ccGGAAAACCGCGTGCTGGGCTTAGTGAGCTACGCGTGGGAAAGCTTTGGCGAGGAGATTGGTACAGTGGTGCTGTTCTCGGTGATGTGGTCACGCATGACGCGTAACGGTGCGCTGGCGGGGCTGATCATAGGTGCGCTGGCGGTTATAGTCTGGAAACAGTTCGGCTGGCTGGGACTATACGAAATTATTCAGGGCTTTATTTTCGGGAGTATTGGGATTGTAGTGTTTAGTTTGTTGGGTAAAGCGcc  >  1:214066/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                 ATTGCCGGGATTCTGCTGTCGGCAATTCTGGCGGCGGTAATGTCAACCTTAAGTTGCCAGCTGCTGGTGTGCTCCAGTGCGATTACCGAAGATTTGTACAAAGCGTTTCTGCGTAAACATGCCAGCCAGAAAGAGCTGGTGTGGGTAGGGCGTGTGATGGTGCTGGTGGTGGCGCTGGTGGCGATTGCGCTGGCGGCAAACCCGGAAAACCGCGTGCTGGGCTTAGTGAGCTACGCGTGGGCAGGCTTTGGCGCGGCGTTTGGTCCAGTGGTGCTGTTCTCGGTGATGTGGTCACGCATGACGCGTAACGGTGCGCTGGCGGGGATGATCATCGGTGCGCTGACGGTTATCGTCTGGAAACAGTTCGGCTGGCTGGGACTGTACGAAATTATTCCGGGCTTTATCTTCGGCAGTATTGGGATTGTAGTGTTTAGTTTGCTGGGTAAAGCGCC  >  NC_000913/1080280‑1080731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑