Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	785,710	C→T	F26F (TTC→TTT)	aroG →	3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	785,710	0	C	T	100.0%	29.8 / NA	11	F26F (TTC→TTT)	aroG	3‑deoxy‑7‑phosphoheptulonate synthase, Phe‑sensitive
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/3);  total (8/3)

CGTTTTTCGCGACAATCTGGCGTTTTTCTTGCTAATTCCAGGATTAATCCGTTCATAGTGTAAAACCCCGTTTACACATTCTGACGGAAGATATAGATTGGAAGTATTGCATTCACTAAGATAAGTATGGCAACACTGGAACAGACATGAATTATCAGAACGACGATTTACGCATCAAAGAAATCAAAGAGTTACTTCCTCCTGTCGCATTGCTGGAAAAATTCCCCGCTACTGAAAATGCCGCGAATACGGTTGCCCATGCCCGAAAAGCGATCCATAAGATCCTGAAAGGTAATGATGATCGCCTGTTGGTTGTGATTGGCCCATGCTCAATTCATGATCCTGTCGCGGCAAAAGAGTATGCCACTCGCTTGCTGGCGCTGCGTGAAGAGCTGAAAGATGAGCTGGAAATCGTAATGCGCGTCTATTTTGAAAAGCCGCGTACCACGGTGGGCTGGAAAGGGCT  >  NC_000913/785487‑785952                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   cGTTTTTCGCGACAATCTGGCGTTTTTCTTGCTAATTCCAGGATTAATCCGTTCATAGTGTAAAACCCCGTTTACACATTCTGACGGAAGATATAGATTGGAAGTATTGCATTCACTAAGATAAGTATGGCAACACTGGAACAGACATGAATTATCAGAACGACGATTTACGCATCAAAGAAATCAAAGAGTTACTTCCTCCTGTCGCATTGCTGGAAAAATTTCCCGCTACTGAAAATGCCGCGAATACg                                                                                                                                                                                                                         <  1:49781/251‑1 (MQ=255)      ttCGCGACAATCTGGCGTTTTTCTTGCTAATTCCAGGATTAATCCGTTCATAGTGTAAAACCCCGTTTACACATTCTGACGGAAGATATAGATTGGAAGTATTGCATTCACTAAGATAAGTATGGCAACACTGGAACAGACATGAATTATCAGAACGACGATTTACGCATCAAAGAAATCAAAGAGTGACGTCCTCCTGTCGCATTGCTGGAAAAATTTCCCGCTACTGAAAATGCCGCGAATACGGTTg                                                                                                                                                                                                                     <  1:163210/250‑1 (MQ=255)                                cTAATTCCAGGATTAATCCGTTCATAGTGTAAAACCCCGTTTACACATTCTGACGGAAGCTATAGATTGGAAGTATTGCATTCACTAAGATAAGTATGGCAACACTGGAACAGACATGAATTATCAGAACGACGATTTACGCATCAAAGAAATCAAAGAGTTACTTCCTCCTGTCGCATTGCTGGCAAAATTTCCCGCTAc                                                                                                                                                                                                                                            >  1:197223/1‑201 (MQ=255)                                                                                     cGGAAGATATAGATTGGAAGTATTGCATTCACTAAGATAAGTATGGCAACACTGGAACAGACATGAATTATCAGAACGACGATTTACGCATCAAAGAAATCAAAGAGTTACTTCCTCCTGTCGCATTGCTGGAAAAATTTCCCGCTACTGAAAATGCCGCGAATACGGt                                                                                                                                                                                                                       >  1:176453/1‑169 (MQ=255)                                                                                                                          tAAGTATGGCAACACTGGAACAGACACGAATTATCAGGCCGCCGATTTGCGCATCAAAGAAAACAAAGACTTACTTCCTCCTGTCGCATTGCTGGAAAAATTTCCGGCTACTGAAAATGCCGCGAATACGGTTGACAATGCCCGAAAAGCGATCCATAAGATCCTGAAAGGTAATGATGATCGCCTGTTGGTTGTGATTGGCCCATGCTCAATTc                                                                                                                                    >  1:94635/1‑215 (MQ=255)                                                                                                                           aaGTATGGCAACACTGAAACAGACATTAATTATCAGAACGACGATTGACGCATCAAAGAAATCAAAGAGTTACTGCCTCCTGTCGCATTGCTGGAAAAATTTCCCGCTACTGAAAATGCCGCGAATACGGTTGCCCATGCCCGAAAAGCGATCCATAAGATCCTTAAAGGTAATTATGATCGCCGGTTGGTCGTGATTGGCCCATGCTCAATTCATGATCCTGTCGCGGCAAAAGAGTATGCCACTCGCt                                                                                                <  1:188568/250‑1 (MQ=255)                                                                                                                                                                                                   nttCCTCCTGTCGCATTGCTGGAAAAATTTCCCGCTACTGAAAATGCCGCGAATACGGTTGCCCATGCCCGAAAAGCGATCCATAAGATCCTGAAAGGTAATGATGATCGCCTGTTGGTTGTGATTGGCCCATGCTCAATTCATGATCCTGTCGCGGCAAAAGAGTATGCCACt                                                                                                    >  1:132136/2‑174 (MQ=255)                                                                                                                                                                                                                cATTGCTGGAAAAATTTCCCGCTACTGAAAATGCCGCGAATCCGGTTGCCCATGCCCGAAAAGCGATCCATAAGATCCTGAAAGGTAATGATGATCGCCTGTTGGTTGTGATTGGCCCATGCTCACTTCATGATCATGTCGCGGCAAAAGAGTATGCCACTCGCTTGCTGGCGCTGCGTGAAGAGCTGAAAGATGAGCTGGAAATCGTAATGCGCGTCTATTTTGAAAAGCCGCGTACCACGGTGGGCTgg          >  1:144176/1‑251 (MQ=255)                                                                                                                                                                                                                cATTGCTGGAAAAATTTCCCGCTACTGAAAATGCCGCGAATACGGTTGCCCATGCCCGAAAAGCGATCCATAAGATCCTGAAAGGTAATGATGATCGCCTGTTGGTTGTGATTGGCCCATGCTCAATTCATGATCCTGTCGCGGCAAAAGAGTATGCCACTCGCTTGCTGGCGCTGCGTGAAGAGCTGAAAGATGAGCTGGAAATCGTAATGCGCGTCTATTTTGAAAAGCCGCGTACCACGGTGGGCTgg          >  1:97310/1‑251 (MQ=255)                                                                                                                                                                                                                     cTGGAAAAATTTCCCGCTACTGAAAATGCCGCGAATACGGTTGCCCATGCCAGAACAGCGATCCATCAGATCCTGACAGGCAATGATGATCGACTGTTGGTTGTGATTGGCCCATGCTCAATTAATGATCCTGTCGCGGCAAAAGAGGATGCCACTCGCTTGCTGGAGCTGCGTGAAGAGCTGAAAGATGAGATGGAAATCGTAATGCGCGACTATTTTg                                    >  1:153866/1‑220 (MQ=255)                                                                                                                                                                                                                        gAAAAATTTCCCGCTACTGAAAATGCCGCGAATACGGTTGCCCATGCCCGAAAAGCGATCCATAAGATCCTGAAAGGTAATGATGATCGCCTGTTGGTTGTGATTGGCCCATGCTCAATTCATGATCCTGTCGCGGCAAAAGAGTATGCCACTCGCTTGCTGGCGCTGCGTGAAGAGCTGAAAGATGAGCTGGAAATCGTAATGCGCGTCTATTTTGAAAAGCCGCGTACCACGGTGGGCTGGAAAGGGCt  >  1:17444/1‑251 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                   CGTTTTTCGCGACAATCTGGCGTTTTTCTTGCTAATTCCAGGATTAATCCGTTCATAGTGTAAAACCCCGTTTACACATTCTGACGGAAGATATAGATTGGAAGTATTGCATTCACTAAGATAAGTATGGCAACACTGGAACAGACATGAATTATCAGAACGACGATTTACGCATCAAAGAAATCAAAGAGTTACTTCCTCCTGTCGCATTGCTGGAAAAATTCCCCGCTACTGAAAATGCCGCGAATACGGTTGCCCATGCCCGAAAAGCGATCCATAAGATCCTGAAAGGTAATGATGATCGCCTGTTGGTTGTGATTGGCCCATGCTCAATTCATGATCCTGTCGCGGCAAAAGAGTATGCCACTCGCTTGCTGGCGCTGCGTGAAGAGCTGAAAGATGAGCTGGAAATCGTAATGCGCGTCTATTTTGAAAAGCCGCGTACCACGGTGGGCTGGAAAGGGCT  >  NC_000913/785487‑785952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑