Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	775,195	C→T	L15F (CTT→TTT)	tolQ →	Tol‑Pal system protein TolQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	775,195	0	C	T	100.0%	14.8 / NA	8	L15F (CTT→TTT)	tolQ	Tol‑Pal system protein TolQ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/5);  total (3/5)

CGACGATATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAAC‑GCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCT  >  NC_000913/774967‑775339                                                                                                                                                                                                                                      |                                                                                                                                                 cgacgaTATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTG‑CAACCGCCGCGAATACTTTGCTGAATGACGCAGCGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGAGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCTTTCTGGTTAAACTTATCATGtt                                                                                                                            >  1:195410/1‑251 (MQ=255)                                              cACCTCTTTGGTTTTCACGCAACGTATTGTCAAC‑GCCGAGATTACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCTTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTTGGCCATTATTATcc                                                                                <  1:34464/250‑1 (MQ=255)                                                             cACGCAACGTATTGTCAAC‑GCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCTTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTc                                                                 <  1:143141/250‑1 (MQ=255)                                                                                   cGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCTTTCTGGTTAAACTTATCATGTTGATTTTGAGTGGTTTTTCAATCGCATCTTTGGCCATTATTAGCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGcgaagc                                           <  1:137460/251‑1 (MQ=255)                                                                                                  gaatgaaGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCTTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGgcgcgc                                                >  1:15229/1‑231 (MQ=255)                                                                                                                            gCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCTTTCTGGTTAAACTTATCATGTTGATGTTGATTGGTTTTTCAATCGCATCTTTGGCCATTATTATCCAGCTGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAATCGTTTGAAGATAAATTCTGGTCTGGTATCGAActc   <  1:176837/250‑1 (MQ=255)                                                                                                                                                              tCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCGTAATGCTATCTTTCTGGTTAAACTGATCATGTTGATTTTGATTGGTTTTTCAAGCGCGGCTTGTGCCATTATTATCCAGCGGACCCGGATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATtctggtctg              <  1:155842/205‑1 (MQ=255)                                                                                                                                                              tCCCAAGGNTNNNGNNGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCNTGACGGCTAGCTTTCNGGNTAAANTTANCATGTTGATTTTGATTGGTTTTTCAATCGCGTCTTGGGCCATTATTCTCAAGCGGACCCGTATTCTTAACGAAGCGGCGCGCGAAGCCGAAGCGTGTGACGAAAANTtcnggactnngngcggcannn  >  2:155842/1‑201 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                 CGACGATATGCTCGAAATACAGACTGAAATAACATCAATGCGTGGCACCTCTTTGGTTTTCACGCAACGTATTGTCAAC‑GCCGAGAATACTTTGCTGAATGAAGCAGAGGTTCTGGTTGTTTGCGTTGACCCACTCAAAATGAAGCCTCGTGCGCTTCCCAAGTCTATTGTCGCGGAGTTTAAGCAGTGACTGACATGAATATCCTTGATTTGTTCCTGAAGGCTAGCCTTCTGGTTAAACTTATCATGTTGATTTTGATTGGTTTTTCAATCGCATCTTGGGCCATTATTATCCAGCGGACCCGTATTCTTAACGCAGCGGCGCGCGAAGCCGAAGCGTTTGAAGATAAATTCTGGTCTGGAATCGAACTCT  >  NC_000913/774967‑775339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑