Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	759,162	C→T	L153F (CTC→TTC)	sucA →	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	759,162	0	C	T	80.0%	16.8 / NA	10	L153F (CTC→TTC)	sucA	subunit of E1(0) component of 2‑oxoglutarate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (5/3);  minor base G (0/2);  total (5/5)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAC‑CATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGC‑AAAATTCC  >  NC_000913/758946‑759369                                                                                                                                                                                                                          |                                                                                                                                                                                                                 tcCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAC‑CATGAAAGTCGGCGAgn                                                                                                                                                                                                         <  1:61934/226‑2 (MQ=255)          gACACCTATGTGAAGCAGGGTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAC‑CATGAAATTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCggn                                                                                                                                                                         <  1:61908/249‑2 (MQ=255)                       aGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTGGCCAGCGGCAAAGAAAC‑CATGAAATTCTGCGAGCTGCTGGAAGCCCTCAAGCAAGCCTACTGCGGCCCGATTGGTGCCGAg                                                                                                                                                          <  1:116572/251‑1 (MQ=255)                         cAGGTTAAAGGCCTGCAGCTCATTAACGCATCCCGCTTCCGTGGTCACCAGCAGGCGAATCTCGATCCGCTGGGACTGTGGCAGCAATATAAATGGGCCGATCTGGATCCGTCTTTCCACTATCTGACCGAAGCAGACGTCCAGGAGACCTTGAGCGGCGGTTCGTTTGCCAGCGGCAAAGAAAC‑CATGAAAGTCGGCAAGCTGCTGGAAGCGGTCAAGCAAACCTTCTGCGGCCCGATTGGTGCCGAGta                                                                                                                                                        <  1:101522/251‑1 (MQ=255)                                             cATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAC‑CATGAAATTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCg                                                                                                                                    >  1:64367/1‑251 (MQ=255)                                                 aaCGCATCCCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAC‑CATGAAATTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCgaan                                                                                                                                 <  1:130883/250‑2 (MQ=255)                                                                        ncAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGCTAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAC‑CATGAAATTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGt                                                                                                          >  1:19805/2‑250 (MQ=255)                                                                                cGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCAACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAACGAAAC‑CATGAAATTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTgg                                                                                                                      >  1:33912/1‑230 (MQ=255)                                                                                                            gcaAGATAAAGTGGCCGATGTGGATCCGTGTTTACACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAC‑CATGAAATTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAag                                                                        >  1:172206/1‑248 (MQ=255)                                                                                                                                                                              cTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAACACA‑GAAATTCGGGGAGATGGAGGAAGACCTCAAGCAAACCTACTGAGGCCCGATTGGTGGCGAGAATATGCACATTACCACCCCCCAAGCAAAAAGCAGGCACCCACAGGGTATCGAGTCTGGTCGCGCGACTTTCAATACCGAAGAGAAAAACAGCTTCTTAAGCGAACTGACCGCCCCCGAAGGACTTGAACGTTAACTCGG‑GCGAAAATTcc  >  1:209040/1‑251 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                 TCCGACCCTGACACCAATGTGAAGCAGGTTAAAGTCCTGCAGCTCATTAACGCATACCGCTTCCGTGGTCACCAGCATGCGAATCTCGATCCGCTGGGACTGTGGCAGCAAGATAAAGTGGCCGATCTGGATCCGTCTTTCCACGATCTGACCGAAGCAGACTTCCAGGAGACCTTCAACGTCGGTTCATTTGCCAGCGGCAAAGAAAC‑CATGAAACTCGGCGAGCTGCTGGAAGCCCTCAAGCAAACCTACTGCGGCCCGATTGGTGCCGAGTATATGCACATTACCAGCACCGAAGAAAAACGCTGGATCCAACAGCGTATCGAGTCTGGTCGCGCGACTTTCAATAGCGAAGAGAAAAAACGCTTCTTAAGCGAACTGACCGCCGCTGAAGGTCTTGAACGTTACCTCGGCGC‑AAAATTCC  >  NC_000913/758946‑759369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑