Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	697,786	G→A	T464T (ACC→ACT)	asnB ←	asparagine synthetase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	697,786	0	G	A	100.0%	18.4 / NA	8	T464T (ACC→ACT)	asnB	asparagine synthetase B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (2/6);  total (2/6)

ATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACTCAGCGGCGCTCGGAAGCGGGAATAGTTCTTCAAAGATCTCCCGATACAAGTACGCTTCTTTAGAGGTTGGCGTGTTGTACGGGAAGCGGAAGCGGGCAGTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCACTTCTTTCAGGGTGTCGATCCAACTGTAACCGACGCCATCGGAGAACTGCTCTTTCTGCCGCCAGGCCACGCTTGCAGGCAGATACGCTTCAAAACATTCACGCAGGATGTGTTTTTCCATTTTGCCGTTACCGCACATTTTATCCTGTGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTG  >  NC_000913/697587‑697979                                                                                                                                                                                                        |                                                                                                                                                                                                  aTTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACTCAGCGGCGCTCGGAAGCGGGAATAGTTCTTCAAAGATCTCCCGATACAAGTACGCTTCTTTAGAGGTTGGCGTGTTGTACGGGAAGCGGAAGCTGGCAGTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCACTTCTTTCAGAGTGTCGATCCAACTGTAACTGACTCCATCGTAGAACTGCTCTTTCTgccgc                                                                                                                                                <  1:176699/251‑1 (MQ=255)                                         gCCCGGCACGCACTCAGCGGCGCTCGGAAGCGGGAATAGTTCTTCAAAGATCTCCCGATACAAGTACGCTTCTTTAGAGGTTGGCGTGTTGTACGGGAAGCGGAAGCGGGCAGTTTCCAGTTGCTGAGCAGAAACCTGCTGCGCAGCCACTTCTTTCAGAGTGTCGATCCAACTGTAACCGACGCCATCGGAGAACTGCTCTTTCTGCCGCCAGGCCACGCTTGCAGGCAGATACGCTTCAAAACATTCAc                                                                                                        >  1:163776/1‑251 (MQ=255)                                              gcacgcacTCAGCGGCGCTCGGAAGCGTGAATAGTTCTTCAAAGATCTCCCGATACAAGTACGCTTCTTTAGAGGTTGGCGTGTTGTACGGGAAGCGGAAGCGGGCAGTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCACTTCTTTCAGAGTGTCGCTCCAACTGTAACCGACGCCATCGGAGAACTGCTCTTTCTGCCGCCAGGCCACGCTTGCAGGCAGATACGCTTCAAAACATTCACGCAgg                                                                                                   >  1:93338/1‑251 (MQ=255)                                                         gcggcgCTCGGAAGCGGGAATAGTTCTTCAAAGATCGCCCGATACAAGTACGCGTCTTTAGAGGTTGGCGTGTTGTACGGGAAGCGGAAGCGGGCAGTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCACTTCTTTCAGAGTGTCGATCCAACTGTAACCGACGCCATCGGAGAACTTCTCTTTCTGCCGCCAGGCCACGCTTGCAGGCAGATACGCTTCAAAACATTCACGCAGGATGTGTTTTTc                                                                                        <  1:107527/251‑1 (MQ=255)                                                                 cGGAAGCGGGAATAGTTCTTCAAAGATCTCCCGATACAAGTACGCTTCTTTAGAGGTTGGCGTGTTGTACGGGAAGCGGAAGCGGGCATTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCCCTTCTTTCAGAGTGTCGATCCAACTGTAACCGACGCCATCGGAGAACTGCTCGTTCGGCCGCCAGGCCACGCTTGCAGGCAGATACGCTTCAAAACATTCACGCAGGATGTGTTTTTCCATTTTGc                                                                                <  1:156525/251‑1 (MQ=255)                                                                                 tnnnnNAAGNTCTCCCGATACAAGTACGCTTCTTTAGAGGTTGGCGTGGTGTACGGGAAGCGGAAGCGGGCAGTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCACTTCTTGCAGAGTGTCGATCCAACTGTAACCGAc                                                                                                                                                                            <  2:131879/143‑1 (MQ=255)                                                                                                                              gtgtTGTACGGGAAGCGGAAGCGGGCAGTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCACTTCTTTCAGAGTGTCGATCCAACTGTAACCGACGCCATCGGAGAACTGCTCTTTCTGCCGCCAGGCCACGCTTGCAGGCAGATACGCTTCAAAACATTCACGCAGGATGTGTTTTTCCATTTTGCCGTTACCGCACATTTTATCCTGTGGGTTAATACGCATCGCCACATCAAGGAAtt                          <  1:85869/244‑1 (MQ=255)                                                                                                                                               gaagcggGCAGTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCACTTCTTTCAGAGTGTCGATCCAACTGTAACCGACGCCATCGGAGAACTGCTCTTTCTGCCGCCAGGCCACGCTTGCAGGCAGATACGCTTCAAAACATTCACGCAGGATGTGTTTTTCCATTTTGCCGTTACCGCACATTTTATCCTGTGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTg  <  1:12388/251‑1 (MQ=255)                                                                                                                                                                                                        |                                                                                                                                                                                                  ATTCGATCGCTTTAGCGGAAGAACAAGCGACGGAAGGACCGCCCGGCACGCACTCAGCGGCGCTCGGAAGCGGGAATAGTTCTTCAAAGATCTCCCGATACAAGTACGCTTCTTTAGAGGTTGGCGTGTTGTACGGGAAGCGGAAGCGGGCAGTTTCCAGTTGCTGATCAGAAACCTGCTGCGCAGCCACTTCTTTCAGGGTGTCGATCCAACTGTAACCGACGCCATCGGAGAACTGCTCTTTCTGCCGCCAGGCCACGCTTGCAGGCAGATACGCTTCAAAACATTCACGCAGGATGTGTTTTTCCATTTTGCCGTTACCGCACATTTTATCCTGTGGGTTAATACGCATCGCCACATCAAGGAATTTTTTGTCGAGGAACGGAACGCGTG  >  NC_000913/697587‑697979

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑