Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	681,686	C→T	intergenic (+23/+37)	djlC → / ← hscC	co‑chaperone DjlC/chaperone protein HscC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	681,686	0	C	T	100.0%	18.0 / NA	9	intergenic (+23/+37)	djlC/hscC	co‑chaperone DjlC/chaperone protein HscC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/6);  total (3/6)

CCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTCTCGCTCGTCACCCAG  >  NC_000913/681454‑681854                                                                                                                                                                                                                                         |                                                                                                                                                                         ccATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGTCGGATGCAGCGTTTAcgc                                                                                                                                                        >  1:48935/1‑251 (MQ=255)               tGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGTCGGATGCAGCTTTTACGCCGCATCCGGCAgn                                                                                                                                           <  1:174686/250‑2 (MQ=255)                       ggATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATGATCAAAATCATTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTCTTGCGCCGATGTCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTAt                                                                                                                                  <  1:96467/251‑1 (MQ=255)                                    gAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGAGTAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGAGGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGTCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATgg                                                                                                                     <  1:186768/251‑1 (MQ=255)                                                                      gATGAGCCAACGCCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGTCGGATGCAGCGTTGACGCCGCATCCGGCAGTCGTGGTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATc                                                                                   <  1:126199/251‑1 (MQ=255)                                                                                                          gatgaGGGTGAAAAACCACCGAGCATTACCAGCATTATCCAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGTCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCACTGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATc                                               >  1:66801/1‑251 (MQ=255)                                                                                                                                    tACCTGCATTATCAAATTCAGTTTAACGGTGCTGGTGATAGGTTAGGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACTATGTTTTTTTTGCGCCGATGTCGGATGCAGCGTTTCCGCCGCATCCGGTAGCCGTGTTTATGGGATCTCAATGTCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACCCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGAtc                      <  1:124937/250‑1 (MQ=255)                                                                                                                                                                                 tGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGTCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCccgccgcc                                                                                             >  1:16305/1‑134 (MQ=255)                                                                                                                                                                                                                           tttttNCGCCGNTNNCGGATGCAGCGTTTACGCCNCATCCGGCAGCCGTGNTTATNGGATCNCANtn                                                                                                                      >  2:82510/1‑66 (MQ=255)                                                                                                                                                                                                                           gttttGCGCCGATGTCGGAGGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTTACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTCTCGCTCGGCACCCAg  <  1:82510/182‑1 (MQ=255)                                                                                                                                                                                                                                         |                                                                                                                                                                         CCATTAGAGGCCATTGTCACCCGGATGCTTTTGACGAAAGTGAAACTCGAGCCGGAGGATGAAGATGTCGATGAGCCAACACCTGAAAATCATGAAGAAAAAAATGATGAGGGTGAAAAACCACAGAGCATTACCAGCATTATCAAAATCAGTTTAACGGTGCTGGTGATAGGTTATGCTCTCGGCAAAATCGCGATGTTGTTTAGCTGACGATGTGTTTTTTGCGCCGATGCCGGATGCAGCGTTTACGCCGCATCCGGCAGCCGTGTTTATGGGATCTCAATGGCTAAATAATCGCTCGCCCGCCGCCGAACTTCATCCACGCGGGCCATATCGTTTGACTGCAACGCCGCATCAAAATCGGTGATCACCCGGCCGATCTCTTCTCGCTCGTCACCCAG  >  NC_000913/681454‑681854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑